Method for evaluating male infertility by combining sperm DNA fragmentation index, reactive oxygen species and 8-hydroxydeoxyguanosine

A hydroxydeoxyguanosine, male infertility technology, applied in the preparation of test samples, material inspection products, instruments, etc., can solve the problem of not reflecting the functional characteristics of sperm, and achieve the effect of accurate functional level

Inactive Publication Date: 2018-12-25
广东省计划生育科学技术研究所
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Problems solved by technology

Existing indicators for assessing semen quality rely on traditional semen routine analysis. Semen routine analysis only detects indicators such as sperm quantity, viability, and morphology. Th

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  • Method for evaluating male infertility by combining sperm DNA fragmentation index, reactive oxygen species and 8-hydroxydeoxyguanosine
  • Method for evaluating male infertility by combining sperm DNA fragmentation index, reactive oxygen species and 8-hydroxydeoxyguanosine
  • Method for evaluating male infertility by combining sperm DNA fragmentation index, reactive oxygen species and 8-hydroxydeoxyguanosine

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Embodiment Construction

[0011] 1. After resuscitating the frozen semen specimen, test the sperm concentration and motility according to the requirements of the "World Health Organization Laboratory Manual for Human Semen Examination and Processing" (5th edition). 90 cases of frozen semen samples, of which 60 cases are the semen samples of 60 fertile persons, which is the fertility group; 30 cases are the semen samples of the infertile persons, which is the infertility group.

[0012] 2. Sperm DFI, ROS and 8-OHdG detection

[0013] Sperm chromatin structure assay (SCSA) was used to detect DFI of sperm from infertile and fertile groups. The main components of liquid A are: 0.01mol / L Tris-HCl, 0.15mol / L NaCl, 1mmol / L EDTA; liquid B is 0.08mol / L Hcl, 0.5mol / L NaCl, 0.1% Trition X-100; liquid C It is 37mmol / L citric acid, 0.126mol / L Na2HPO4, 1mmol / LEDTA, 0.15mol / L NaCl, 1mg / ml AO. Measure 10,000 sperm per sperm suspension by flow cytometry, calculate the proportion of red fluorescent sperm, and calculat...

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Abstract

The invention relates to a method for evaluating male infertility by combining sperm DNA fragmentation index, reactive oxygen species and 8-hydroxydeoxyguanosine. The method comprises the steps of: determining levels of sperm DNA fragmentation index (DFI), reactive oxygen species (ROS) and 8-hydroxydeoxyguanosine (8-PHdG) from semen samples; correlating levels of the sperm DNA fragmentation index,reactive oxygen species and 8-hydroxydeoxyguanosine with sperm function quality. With sperm DFI=19.26%, ROS=1.91ng/ml and 8-OHdG=50.76ng/l as judgment critical values, the sperm is judged to be infertile when the sperm DFI, ROS and 8-OHdG levels are higher than the critical values.

Description

technical field [0001] The invention relates to a method for jointly evaluating male infertility by sperm DNA fragmentation rate, active oxygen and 8-hydroxydeoxyguanosine. Background technique [0002] Male infertility afflicts about 10%-15% of couples of reproductive age worldwide. Existing indicators for assessing semen quality rely on traditional semen routine analysis. Semen routine analysis only detects indicators such as sperm quantity, viability, and morphology. These indicators only reflect basic semen quality and cannot reflect sperm functional characteristics. The routine is normal, and the woman's factors are also normal, but she can't get pregnant. In order to better measure male fertility and predict reproductive outcomes, it is clinically necessary to accurately assess sperm function. The influence of sperm DNA integrity on sperm function has been widely concerned. Sperm DNA fragmentation index (DFI), reactive oxygen species (Reactive oxgen species, ROS) and...

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Application Information

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IPC IPC(8): G01N1/30G01N33/53
CPCG01N1/30G01N33/53G01N2800/367
Inventor 秦卫兵唐运革韩璐刘晓华伍嘉宝
Owner 广东省计划生育科学技术研究所
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