Preparation method of therapeutic antibody for feline distemper

A therapeutic antibody and heat treatment technology, which is applied in the field of preparation of feline distemper therapeutic antibody, can solve the problems of adverse reactions in animals, high cost of preparation of cat-derived anti-FPV hyperimmune serum, and less research

Pending Publication Date: 2018-12-28
GUANGXI VETERINARY RES INST
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for sick animals, there is no effective and reliable drug treatment except injection of monoclonal antibodies and highly immune serum.
Although antiviral hyperimmune serum is a relatively safe immune preparation, due to the limited content of cat blood, the preparation cost of cat-derived anti-FPV hyperimmune serum is high, and the direct use of homologous hyperimmune serum will easily cause animal i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of therapeutic antibody for feline distemper
  • Preparation method of therapeutic antibody for feline distemper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] One, the preparation of FPV inactivated vaccine

[0032] The FPV-GX01 strain was synchronously inoculated into FK81 cat kidney cells at a volume of 1 / 10, at 37°C CO 2 After standing in the incubator for 24 hours, discard the growth medium and replace it with a maintenance medium to continue culturing. Observe the cell changes every day. When 75% of the cells become diseased, collect the cells, freeze and thaw 3 times, and then centrifuge at 12,000 rpm for 15 minutes at 4°C to remove Cell debris, collect supernatant, determine its TCID 50 / 0.1mL value ≥ 5.0, hemagglutination titer ≥ 1:2 10 Finally, add thymosin to a final concentration of 0.3mg / mL, add formaldehyde to a final concentration of 0.1%, inactivate at 37°C for 24 hours, shake once every 4 hours, then add 4% Tween-80 as the water phase; Span80 and No. 10 white oil was mixed as the oil phase at a volume ratio (V / V) of 3:47, and the water phase and the oil phase were mixed at a volume ratio of 1:2 for water-in-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a therapeutic antibody for feline distemper. The therapeutic antibody for feline distemper is prepared from an FPV inactivated vaccine and an FPV subunit vaccine as immunogensby horse immunization. Therefore, a corresponding preparation method is established; according to the method, two different types of FPV immunogens are creatively inoculated to a healthy horse according to a certain program, the FPV inactivated vaccine is taken as a basic immunogen, the FPV subunit vaccine is taken as an enhanced immunogen, hyperimmune blood is collected after a high-potency antibody is produced, and high-activity immune globulin F(ab')2 is prepared by IgG purification and Fc' segment excision. With the application of the method, a large amount of horse-derived anti-FPV hyperimmune serum can be prepared, and the high-potency F(ab')2 antibody is extracted and purified. A test indicates that the prepared therapeutic antibody for feline distemper not only has higher potency,but also has exact feline distemper preventing and treatment effects, can be used for emergency prevention and treatment of feline distemper and has important practical significance and broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of feline distemper treatment, in particular to a method for preparing a feline distemper therapeutic antibody. Background technique [0002] Feline distemper, also known as feline panleukopenia, feline infectious enteritis, etc., is a fatal contagious infection caused by feline parvovirus (FPV), characterized by high fever, vomiting, sharp decrease in white blood cells and enteritis in felines sick. FPV can directly or indirectly infect a variety of animals such as cats, procyonidae and mustelidae under natural conditions. Animals and minks are the most susceptible, and FPV is often endemic. The fatality rate of cats infected with FPV is generally 60% to 70%, and the highest can reach more than 90%. The harm is extremely serious. [0003] FPV is a parvovirus that relies on host cells for replication. VP1 and VP2 are its structural proteins, which together constitute the main viral capsid protein of FPV vi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K16/08C07K16/06C07K1/30C07K14/015C12N15/35C12N15/866
CPCC07K14/005C07K16/065C07K16/081C07K2317/20C07K2317/35C07K2317/54C12N15/86C12N2710/14043C12N2750/14322C12N2800/22
Inventor 吴健敏刘金凤张振江覃绍敏白安斌唐海波陈凤莲马玲秦树英林俊杨磊
Owner GUANGXI VETERINARY RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap