Preparation method of therapeutic antibody for feline distemper

Abstract

The invention discloses a therapeutic antibody for feline distemper. The therapeutic antibody for feline distemper is prepared from an FPV inactivated vaccine and an FPV subunit vaccine as immunogensby horse immunization. Therefore, a corresponding preparation method is established; according to the method, two different types of FPV immunogens are creatively inoculated to a healthy horse according to a certain program, the FPV inactivated vaccine is taken as a basic immunogen, the FPV subunit vaccine is taken as an enhanced immunogen, hyperimmune blood is collected after a high-potency antibody is produced, and high-activity immune globulin F(ab')2 is prepared by IgG purification and Fc' segment excision. With the application of the method, a large amount of horse-derived anti-FPV hyperimmune serum can be prepared, and the high-potency F(ab')2 antibody is extracted and purified. A test indicates that the prepared therapeutic antibody for feline distemper not only has higher potency,but also has exact feline distemper preventing and treatment effects, can be used for emergency prevention and treatment of feline distemper and has important practical significance and broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of feline distemper treatment, in particular to a method for preparing a feline distemper therapeutic antibody. Background technique [0002] Feline distemper, also known as feline panleukopenia, feline infectious enteritis, etc., is a fatal contagious infection caused by feline parvovirus (FPV), characterized by high fever, vomiting, sharp decrease in white blood cells and enteritis in felines sick. FPV can directly or indirectly infect a variety of animals such as cats, procyonidae and mustelidae under natural conditions. Animals and minks are the most susceptible, and FPV is often endemic. The fatality rate of cats infected with FPV is generally 60% to 70%, and the highest can reach more than 90%. The harm is extremely serious. [0003] FPV is a parvovirus that relies on host cells for replication. VP1 and VP2 are its structural proteins, which together constitute the main viral capsid protein of FPV vi...

AI Technical Summary

Problems solved by technology

However, for sick animals, there is no effective and reliable drug treatment except injection of monoclonal antibodies and highly immune serum.

Although antiviral hyperimmune serum is a relatively safe immune preparation, due to the limited content of cat blood, the preparation cost of cat-derived anti-FPV hyperimmune serum is high, and the direct use of homologous hyperimmune serum will easily cause animal infection due to its low purity. Adverse reactions may occur, and it may also cause the spread of certain infectious diseases due to the presence of other pathogens in the serum. Therefore, the development of high-purity heterologous anti-FPV antibodies has become the best choice for the treatment of feline distemper

At present, there are few domestic studies on antibodies against heterologous feline distemper

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