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Method for improving recovery rates of cellulase

A technology of cellulase and recovery rate, which is applied in the field of improving the recovery rate of cellulase, can solve the problems of cellulase waste, etc., and achieve the effects of reducing adsorption, improving recovery rate, and improving recovery efficiency

Inactive Publication Date: 2018-12-28
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the degradation process of lignocellulose, a large amount of cellulase is adsorbed by lignin, and the re-adsorption method can only adsorb free cellulase, and a large amount of cellulase is wasted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0021] Enzymolysis: substrate addition 1%, cellulase addition 25 FPU / g cellulose, rotation speed 180pm, temperature 50°C, time 48h.

[0022] (2) Solid-liquid separation of the enzymatic hydrolysis solution: filter the enzymatic hydrolysis solution with an aqueous membrane, and repeatedly wash the filter residue with sodium citrate buffer solution containing 0.5% surfactant PEG6000. At the same time, the filter residue was washed repeatedly with a sodium citrate buffer without surfactant.

[0023] (3) Enzymolysis again: Use the filtrate obtained in step 2 to concentrate to the same amount of cellulase as in step (1), and add it to the new enzymolysis system as cellulase, other conditions of the new enzymolysis system Same as step (1).

[0024] (4) Calculate the enzymatic hydrolysis efficiencies of step (1) and step (3) respectively, based on the conversion rate of dextran. Calculation of recovery = second enzymatic hydrolysis efficiency / first enzymatic hydrolysis efficiency. ...

Embodiment example 2

[0026] Enzymolysis: substrate addition 5%, cellulase addition 30FPU / g cellulose, rotation speed 200rpm, temperature 50°C, time 48h.

[0027] (2) Solid-liquid separation of the enzymatic hydrolysis solution: filter the enzymatic hydrolysis solution with an aqueous membrane, and repeatedly wash the filter residue with sodium citrate buffer solution containing 0.1% surfactant PEG4000. At the same time, the filter residue was washed repeatedly with a sodium citrate buffer without surfactant.

[0028] (3) Enzymolysis again: Use the filtrate obtained in step 2 to concentrate to the same amount of cellulase as in step (1), and add it to the new enzymolysis system as cellulase, other conditions of the new enzymolysis system Same as step (1).

[0029] (4) Calculate the enzymatic hydrolysis efficiencies of step (1) and step (3) respectively, based on the conversion rate of dextran. Calculation of recovery = second enzymatic hydrolysis efficiency / first enzymatic hydrolysis efficiency. ...

Embodiment example 3

[0031] Enzymolysis: substrate addition 7%, cellulase addition 30FPU / g cellulose, rotation speed 220rpm, temperature 50°C, time 48h.

[0032] (2) Solid-liquid separation of the enzymatic hydrolysis solution: filter the enzymatic hydrolysis solution with an aqueous membrane, and repeatedly wash the filter residue with sodium citrate buffer solution containing 0.1% surfactant PEG8000. At the same time, the filter residue was washed repeatedly with a sodium citrate buffer without surfactant.

[0033] (3) Enzymolysis again: Use the filtrate obtained in step 2 to concentrate to the same amount of cellulase as in step (1), and add it to the new enzymolysis system as cellulase, other conditions of the new enzymolysis system Same as step (1).

[0034] (4) Calculate the enzymatic hydrolysis efficiencies of step (1) and step (3) respectively, based on the conversion rate of dextran. Calculation of recovery = second enzymatic hydrolysis efficiency / first enzymatic hydrolysis efficiency. ...

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PUM

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Abstract

The invention discloses a method for improving recover rates of cellulase. The method comprises the following main steps of: (1) carrying out enzymolysis on cellulose with a cellulase load of 25 FPU / gby taking alkali treated wheat straws as a substrate; (2) filtering enzymatic hydrolysate after the enzymolysis, and flushing filter residues by using sodium citrate with a surfactant; (3) concentrating the obtained filtrate into a volume of enzyme liquid in the step (1) and carrying out equal-system enzymolysis; and (4) calculating a ratio of the second enzymolysis efficiency to the first enzymolysis efficiency, namely a recovery rate. The method is simple to operate and low in cost, and is capable of remarkably improving the recovery rates of cellulase.

Description

[0001] The invention belongs to the field of biofuels and relates to a method for improving the recovery rate of cellulase. Background technique [0002] The preparation of bioethanol from lignocellulose is one of the most potential production routes for the development of green and sustainable energy, and it is also one of the most effective methods to solve resource, environmental and energy problems. However, cellulase has become a major obstacle in the commercialization of cellulosic ethanol due to its low activity, high consumption and high cost. At present, the activity and yield of cellulase have been improved through strain transformation, enzyme directional evolution, and improvement of enzyme stability and tolerance, and the cost of cellulase in the production of cellulosic ethanol has been reduced, but it still cannot meet Economic requirements for lignocellulosic ethanol production. Therefore, in order to significantly reduce the cost of cellulase, the development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14
CPCC12P7/10C12P19/14C12P2201/00Y02E50/10
Inventor 钟成李栋梁舒月力贾士儒谢燕燕
Owner TIANJIN UNIV OF SCI & TECH