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Immunomagnetic nano-particle for visual capture and release of circulating tumor cells, and preparation method thereof

A magnetic nanoparticle and tumor cell technology, which is applied in the field of specific capture and release of circulating tumor cells, can solve problems such as low cell recovery rate, damage to cell structural integrity, and disturbance of cell microenvironment

Active Publication Date: 2018-12-28
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Zhang et al. used a 1 cm × 1 cm capture matrix and captured CTCs as electrodes, and used electrochemical stimulation to release the CTCs on the matrix, which could achieve a release rate of about 50% in whole blood; the release method cell recovery rate Relatively low, and electrical stimulation may damage the integrity of cell structure, disrupt the cell microenvironment, etc.

Method used

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  • Immunomagnetic nano-particle for visual capture and release of circulating tumor cells, and preparation method thereof
  • Immunomagnetic nano-particle for visual capture and release of circulating tumor cells, and preparation method thereof
  • Immunomagnetic nano-particle for visual capture and release of circulating tumor cells, and preparation method thereof

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Embodiment 1

[0062] In this embodiment, the preparation of immunomagnetic nanoparticles for visual capture and release of circulating tumor cells includes the following steps:

[0063] (1) Preparation of fluorescent magnetic nanoparticles MLNs

[0064] Prepare the NaCl solution with a polyacrylamide (PAH) concentration of 0.5 mg / mL, the NaCl solution with a hyaluronic acid (HA) concentration of 0.5 mg / mL, the CdSSe / ZnS aqueous solution with a CdSSe / ZnS concentration of 0.05 mg / mL, and NaCl The NaCl aqueous solution with a concentration of 2.9 mg / mL, the NaCl concentration in the NaCl solution washing liquid of polyacrylamide (PAH) is 2.9 mg / mL, and the NaCl concentration in the NaCl solution of hyaluronic acid (HA) is 2.9 mg / mL. Prepare each solution of the configuration according to the following steps to prepare fluorescent magnetic nanoparticles MLNs:

[0065] (11) to fill with 0.5mg magnetic nanoparticles Fe 3 o 4 Add 4 mL of NaCl aqueous solution containing PAH in the container, th...

Embodiment 2

[0102] In this embodiment, the preparation of immunomagnetic nanoparticles for visual capture and release of circulating tumor cells includes the following steps:

[0103] (1) Preparation of fluorescent magnetic nanoparticles MLNs

[0104] Prepare NaCl solution with polyacrylamide (PAH) concentration of 1.0 mg / mL, NaCl solution with polyacrylamide (PAH) concentration of 1.2 mg / mL, NaCl aqueous solution with hyaluronic acid (HA) concentration of 1.0 mg / mL, CdSSe / ZnS CdSSe / ZnS aqueous solution with a concentration of 0.1mg / mL (solution C) and NaCl aqueous solution with a NaCl concentration of 8.8mg / mL, the NaCl concentration in the NaCl solution of polyacrylamide (PAH) is 8.8mg / mL, hyaluronic acid The NaCl concentration in the NaCl solution of the acid (HA) was 8.8 mg / mL. Prepare each solution of the configuration according to the following steps to prepare fluorescent magnetic nanoparticles MLNs:

[0105] (11) to fill with 0.5mg magnetic nanoparticles Fe 3 o 4 Add 4mL of Na...

Embodiment 3

[0116] In this embodiment, the preparation of immunomagnetic nanoparticles for visual capture and release of circulating tumor cells includes the following steps:

[0117] (1) Preparation of fluorescent magnetic nanoparticles MLNs

[0118] Prepare NaCl solution with polyacrylamide (PAH) concentration of 1.3mg / mL, NaCl solution with polyacrylamide (PAH) concentration of 1.5mg / mL, NaCl solution with hyaluronic acid (HA) concentration of 1.5mg / mL, CdSSe / ZnS CdSSe / ZnS aqueous solution with a concentration of 0.15mg / mL (solution C) and NaCl aqueous solution with a NaCl concentration of 5mg / mL, the NaCl concentration in the NaCl solution of polyacrylamide (PAH) is 5mg / L, hyaluronic acid The NaCl concentration in the NaCl solution of the acid (HA) was 5 mg / mL. Prepare each solution of the configuration according to the following steps to prepare fluorescent magnetic nanoparticles MLNs:

[0119] (11) to fill with 0.5mg magnetic nanoparticles Fe 3 o 4 Add 4mL of NaCl aqueous soluti...

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Abstract

The invention discloses an immunomagnetic nano-particle with fluorescent properties and a preparation method and application thereof. The method comprises the following steps: using magnetic nano-particle Fe3O4 as a core, constructing a magnetic nano-particle with fluorescence properties by depositing a quantum dot layer on the surface of the magnetic nano-particle Fe3O4, and further introducing the anti-epithelial cell adhesion molecule antibody and the aminated polyethylene glycol molecule into the fluorescent magnetic nano-particle to obtain an immunomagnetic nano-particle. The immunomagnetic nano-particle enables rapid specific capture and release of circulating tumor cells. In addition, the quantum dot-labeled magnetic nano-particle enables visualization of capture and release processes of circulating tumor cells.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to the specific capture and release technology of circulating tumor cells. Background technique [0002] Cancer metastasis is the main cause of cancer death, and circulating tumor cells (Circulating tumor cells, CTCs) that metastasize from tumors and enter the blood circulation system are considered to be closely related to cancer metastasis. Remarkably, the number of CTCs in the peripheral blood of tumor patients has a strong correlation with the therapeutic effect and patient survival rate. Therefore, the detection and counting of CTCs can be used to predict whether cancer metastasizes, monitor the course of treatment, and assess the prognosis of cancer. However, the incidence of CTCs in peripheral blood is extremely low, and there are only one to hundreds of CTCs in billions of blood cells. Therefore, reliable and efficient isolation of CTCs is essential for the study of CTCs...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/574H01F1/11
Inventor 易强英周小熙吴尧马少华康珂
Owner SICHUAN UNIV
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