Preservation liquid for isolated deciduous tooth and preparation method thereof

A technology for preserving liquid and deciduous teeth, which is applied in the field of cell engineering, which can solve the problems of pollution and easy damage to microorganisms, and achieve the effects of long-term storage, avoiding microbial contamination, and strong protection

Active Publication Date: 2019-01-01
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a preservation solution for deciduous teeth in vitro, which can effectively solve the problems of easy damage and microbial contamination when deciduous teeth are preserved in vitro

Method used

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  • Preservation liquid for isolated deciduous tooth and preparation method thereof
  • Preservation liquid for isolated deciduous tooth and preparation method thereof
  • Preservation liquid for isolated deciduous tooth and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] An isolated deciduous tooth preservation solution, the preservation solution contains 1g of glucose, 0.05g of reduced glutathione, 3mL of human serum albumin, 0.5g of amino acid, 1g of Ve, 0.2g of trehalose, 12mL of thermosensitive hydrogel in every 100mL of the preservation solution, 0.5g of penicillin, 0.3g of streptomycin, 0.02g of amphotericin B, and the rest of the solute medium is phosphate buffer with a pH value of 7.4.

[0038] Among them, the preparation method of the temperature-sensitive hydrogel is: mix polyacrylamide, methacrylic acid, acrylic acid and chitosan, then add ammonium persulfate, stir evenly, and after standing for 15 hours, wash the reaction product three times to obtain the temperature-sensitive hydrogel. Glue; wherein, the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and ammonium persulfate is 8:1:0.6:0.15:1.

[0039] The preparation method of this preservation solution is:

[0040] Under aseptic conditions, the a...

Embodiment 2

[0044] An isolated deciduous tooth preservation solution, which contains 0.5g of glucose, 1mL of human serum albumin, 0.05g of reduced glutathione, 0.5g of amino acids, 0.5g of Ve, 0.3g of trehalose, and thermosensitive hydrogel in every 100mL of the preservation solution 20mL, penicillin 0.6g, streptomycin 0.5g, amphotericin B 0.05g, and the rest of the solute medium is phosphate buffer with a pH value of 7.6.

[0045] Among them, the preparation method of the temperature-sensitive hydrogel is: mix polyacrylamide, methacrylic acid, acrylic acid and chitosan, then add sodium persulfate, stir evenly, and after standing for 15 hours, wash the reaction product three times to obtain the temperature-sensitive hydrogel. Glue; wherein, the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and sodium persulfate is 10:0.5:0.8:0.2:0.6.

[0046] The preparation method of this preservation solution is:

[0047] Under aseptic conditions, the above-mentioned componen...

Embodiment 3

[0051] An isolated deciduous tooth preservation solution, the preservation solution contains 1.2g of glucose, 0.04g of reduced glutathione, 5mL of human serum albumin, 0.3g of amino acid, 0.1g of trehalose, 10mL of thermosensitive hydrogel, and 0.2g of penicillin in every 100mL of the preservation solution. g, 0.1 g of streptomycin, 0.01 g of amphotericin B, and the rest of the solute medium is phosphate buffer solution with a pH value of 7.2.

[0052] Among them, the preparation method of the temperature-sensitive hydrogel is: mix polyacrylamide, methacrylic acid, acrylic acid and chitosan, then add lauroyl peroxide, stir evenly, after standing for 15 hours, wash the reaction product three times to obtain temperature-sensitive water Gel; wherein, the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and lauroyl peroxide is 6:0.8:1:0.3:1.

[0053] The preparation method of this preservation solution is:

[0054] Under aseptic conditions, the above-menti...

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Abstract

The invention discloses a preservation liquid for an isolated deciduous tooth and a preparation method thereof. The preservation liquid comprises, by weight, 0.5 to 1.2 parts of glucose, 0.01 to 0.05part of reduced glutathione, 0.1 to 0.5 part of amino acids, 1 to 5 parts of human albumin, 0 to 1 part of Ve, 0.1 to 0.3 part of trehalose, 10 to 20 parts of a temperature-sensitive hydrogel, 0.3 to1.5 parts of an antibacterial drug and 70 to 90 parts of a phosphate buffer solution. The preparation method comprises a step of mixing all the components according to the above formula under an aseptic condition so as to prepare the preservation liquid for the isolated deciduous tooth. The preservation liquid prepared by using the method of the invention can provide nutrients for the pulp tissueof the isolated deciduous tooth, effectively reduce mechanical damage during the transportation of dental pulp, and effectively prolong the preservation time of the isolated deciduous tooth.

Description

technical field [0001] The invention belongs to the technical field of cell engineering, and in particular relates to an isolated deciduous tooth preservation solution and a preparation method thereof. Background technique [0002] Stem cells are a kind of pluripotent cells with self-renewing ability. With the deepening of stem cell research, more and more stem cells from different sources have become research hotspots in various research institutions, such as bone marrow source Mesenchymal stem cells, placenta-derived mesenchymal stem cells, dental pulp-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells, etc. [0003] In 2003, Miura et al. isolated pluripotent stem cells with high monoclonal ability, proliferation ability, and osteogenesis in vivo from the pulp of exfoliated deciduous teeth of children aged 7 to 8, and named them SHED. [0004] Stem cells from human exfoliated decid-uous teeth (SHED) are dental pulp mesenchymal stem cells derived from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 高雪华海泉
Owner CHENGDU QINGKE BIOTECH
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