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Urine glucose content rapid detection kit based on chloroauric acid color reaction and detection method

A detection kit, color reaction technology, applied in the measurement of color/spectral properties, analysis by chemical reaction of materials, and material analysis by observing the impact on chemical indicators, etc., can solve the problem of lack of accurate urine detection. Sugar concentration and other problems, to achieve the effect of improving urine sugar detection efficiency, fast reaction speed and simple steps

Active Publication Date: 2019-01-01
XI'AN PETROLEUM UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There has not yet been a colorimetric reaction using urine sugar to reduce gold ions to determine the concentration of urine sugar qualitatively through colorimetry, nor has there been any relevant research results on the accurate detection of urine sugar concentration using the correlation curve fitting formula between urine sugar concentration and nano-gold particle size

Method used

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  • Urine glucose content rapid detection kit based on chloroauric acid color reaction and detection method
  • Urine glucose content rapid detection kit based on chloroauric acid color reaction and detection method
  • Urine glucose content rapid detection kit based on chloroauric acid color reaction and detection method

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Experimental program
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Effect test

Embodiment 1

[0025] The urine sugar content rapid detection kit of this embodiment consists of a chloroauric acid reagent bottle, a urine sugar concentration color card, and a urine sugar concentration precise quantitative card.

[0026] The chloroauric acid reagent bottle is a one-time sealed glass bottle with 3mL chloroauric acid chromogenic solution. The chloroauric acid chromogenic solution is prepared by adjusting the pH value to 8 with 0.5mM chloroauric acid aqueous solution and 0.2M sodium hydroxide aqueous solution. The one-time capped and sealed glass bottle has a capacity of 8mL, a height of 75mm, and a diameter of 12mm. It is made of screw-top quartz glass, and the bottle cap is made of heat-resistant plastic material, and a heat-resistant leak-proof gasket is installed inside.

[0027] urine sugar concentration color chart figure 1 As shown, it consists of color block A, color block B, color block C, color block D, color block E, color block F, color block G, color block H, and...

Embodiment 2

[0033] The method for rapidly detecting urine sugar content using the kit of Example 1 is as follows:

[0034] Dilute the urine to be tested 20 times with deionized water to obtain the urine dilution to be tested; after preheating the urine dilution to be tested in a boiling water bath, take 200 μL and add it to a chloroauric acid reagent bottle that has been preheated in a boiling water bath for 5 minutes After stirring rapidly for 5 seconds, continue to keep heating in a boiling water bath for 6 minutes, so that the glucose in the urine and chloroauric acid undergo redox color reaction. After the reaction is completed, the color of the nano-gold solution obtained is compared with the urine sugar Compare the color card to judge the range of glucose concentration.

[0035] After judging the concentration range of glucose, take 1.5 mL of the nano-gold solution obtained after the reaction and drop it into a quartz cuvette, use a UV-visible spectrophotometer to detect its absorba...

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Abstract

The invention discloses a urine glucose content rapid detection kit based on a chloroauric acid color reaction and a detection method. The kit comprises a chloroauric acid reagent bottle, a urine glucose concentration color chart and a urine glucose concentration precise quantification chart. According to the kit and the detection method disclosed by the invention, the glucose in urine serves asa reducing agent to reduce tetrachloroauric acid, at different glucose concentrations, the generated nanogold solution shows different colors; the absorption peak wavelength near 520nm of the nanogoldsolution is detected by an ultraviolet and visible spectrophotometer; the glucose content in the urine is detected rapidly, qualitatively and quantitatively according to the color development range of the nanogold solution and the correlation between the wavelength of the absorbance peak and the glucose concentration in the urine, and the detection sensitivity can reach 0.01mM.

Description

technical field [0001] The invention belongs to the technical field of urine sugar detection, and in particular relates to a urine sugar rapid detection kit based on gold ion color development in the oxidation-reduction reaction process, and a method for rapidly completing qualitative and quantitative detection of urine sugar content at one time by using the kit . Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion or impaired biological action, or both. Diabetes is a long-standing hyperglycemia that leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. It is a disease with the most known complications. [0003] At present, the diagnosis and monitoring of diabetes is mainly through the detection of blood sugar and urine sugar concentration. The blood sugar detection generally adopts blood test in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 杨博丑菊屈撑囤
Owner XI'AN PETROLEUM UNIVERSITY
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