New method for identification of cold tolerance of Anthurium andraeanum germplasm
An identification method and a new germplasm technology, applied in the field of plant science, can solve the problems of increasing cold resistance identification cost, instability, and deviation of identification results, and achieve the effects of avoiding cost input, avoiding result deviation, and reducing costs
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Embodiment 1
[0046] The new Anthurium germplasm 'C1' and the varieties 'Alabama', 'Sensation', and 'Fen Champion' in this example are from Shanghai Huahuagang Enterprise Development Co., Ltd. The specific cold resistance identification steps are as follows:
[0047] (1) Preparation materials: 3 Anthurium varieties 'Alabama', 'Sensation', 'Fen Champion' with different cold tolerance and new germplasm 'C1' of Anthurium to be tested, with seedling age of 12 months. The container used for transplanting was a 16cm×12cm flower pot, and the substrate used was imported peat. The 1000-fold diluted "Hua Duoduo No. 1" aqueous solution was applied once every 7 days, and the low temperature stress experiment was carried out one month later.
[0048] (2) Low temperature stress: use an artificial climate incubator to carry out low temperature stress, the light intensity is 15000 Lx, the light / dark time is 12h / 12h every day, the stress treatment temperature is 6°C, and the treatment time is 3 days, and the...
Embodiment 2
[0062] The new Anthurium germplasm 'C2' and the varieties 'Alabama', 'Sensation', and 'Fen Champion' in this example are from Shanghai Huahuagang Enterprise Development Co., Ltd. The specific cold resistance identification steps are as follows:
[0063] (1) Material preparation: 3 varieties of anthurium with different cold tolerance 'Alabama', 'Sensation', 'Fen Champion' and a new germplasm of anthurium to be tested 'C2', the seedling age is 12 months. The container used for transplanting was a 16cm×12cm flower pot, and the substrate used was imported peat. The 1000-fold diluted "Hua Duoduo No. 1" aqueous solution was applied once every 7 days, and the low temperature stress experiment was carried out one month later.
[0064] (2) Low temperature stress: use an artificial climate incubator to carry out low temperature stress, the light intensity is 15000 Lx, the light / dark time is 12h / 12h every day, the stress treatment temperature is 6°C, and the treatment time is 3 days, and ...
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