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A method for cultivating seedlings by using alum root petioles

A technology for cultivating seedlings and petioles, which is applied in the field of plant tissue culture, can solve the problems of easy damage, destruction of mother plant materials, and unsuitable mother plant materials, etc., and achieve the effect of well-developed root system, improved quantity and quality, and strong plant growth

Active Publication Date: 2021-06-29
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

However, these methods are easy to damage or even destroy the mother plant material. Some methods have higher requirements on the period of explant material extraction, and are not suitable for new germplasm with scarce mother plant material, nor are they suitable for transgenic research on alum roots.

Method used

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  • A method for cultivating seedlings by using alum root petioles
  • A method for cultivating seedlings by using alum root petioles
  • A method for cultivating seedlings by using alum root petioles

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Embodiment

[0040] 1.1 Test materials

[0041] Alumroot 'Paris' (Heuchera 'Pairs') sterile vaccine

[0042] 1.2 Induction of callus

[0043] The petioles 0.8 cm below the leaves of the sterile seedlings were cut as explants, inoculated on the induction medium, and cultured in the dark to induce callus. The culture temperature was 24±2°C for 28-40 days.

[0044] The induction medium uses MS as the basic medium, adds 6-BA 0.5mg / L, and adds picloram (Picloram, PIC) 0.5mg / L, sucrose 30g / L, agar powder 6g / L respectively, conventional sterilization Afterwards, it was aliquoted into petri dishes for use.

[0045] After 12 days, the incision of the petiole began to expand, and a small amount of tiny callus was formed. The number of callus was counted after 20 days and 30 days after the inoculation of the explants. After 20 days, there was a high callus induction rate, which was 72.2%. 30d, the callus induction rate was 96.3%, and the morphology was good (see figure 1 ),Depend on figure 1 It ...

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Abstract

A method for cultivating seedlings by using the petiole of the alum root, inducing callus with the petiole of the aseptic seedling of the alum root, and then performing adventitious bud differentiation and proliferation, inducing rooting, obtaining the seedlings of the alum root, and establishing the callus of the alum root petiole Induction and adventitious bud differentiation regeneration system, in the present invention, the callus induction rate of petioles is above 90%, the induced callus has good differentiation ability, and the number of differentiated adventitious buds is large, up to 6-18 , the rooting rate is high, and the root system is strong; the cultivated seedlings are consistent with the traits of the mother plant, and the alum root regeneration system established in the present invention has laid a foundation for the genetic transformation research of the alum root.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for cultivating seedlings by using alum root petioles. Background technique [0002] Heuchera spp. is native to North America. It is a perennial evergreen herbaceous flower of the genus Heuchera in the saxifrage family. It has various varieties and rich leaf colors. It is a rare shade plant with colorful leaves and is widely used in the United States. In garden landscape. [0003] In recent years, alum root has been used by Chinese gardeners in flower borders, flower beds, three-dimensional greening, and also as potted flowers. It has unique ornamental properties, and has strong cold resistance and drought resistance. Swatch', in addition, alum root also has the function of absorbing heavy metals and improving the ecological environment, which has gradually attracted the attention of the market, and the demand for alum root seedlings has gradual...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 殷丽青张永春孙翊蔡友铭田莉庹晓庆杨柳燕陈敏敏李青竹
Owner SHANGHAI ACAD OF AGRI SCI
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