Freeze drying and refrigeration method for storing dormant eggs of eurytemora pacifica for long term

Abstract

The invention discloses a protective agent with dimethyl sulfoxide, maleic acid and sodium hyaluronate, and further discloses a freeze drying and refrigeration method for storing dormant eggs of eurytemora pacifica for a long term. The freeze drying and refrigeration method includes sieving and thoroughly cleaning the dormant eggs and then transferring the dormant eggs into a container with the protective agent; filling the container with circulating nitrogen dried by inert chemical drying agents; transferring the dormant eggs into light-resistant containers after the eggs are dried, and hermetically storing the dormant eggs. The freeze drying and refrigeration method has the advantages that the integrity of matrixes of the dormant eggs of the eurytemora pacifica can be kept by the protective agent, damage to tissue structures of the dormant eggs can be abated, the high activity of the dormant eggs can be kept, and the hatching rate of the dormant eggs can be increased; technologies ofthe freeze drying and refrigeration method for storing the dormant eggs of the eurytemora pacifica for the long term are easy to operate, the freeze drying and refrigeration method is repeatable, safe and reliable and is low in cost, systems are stable and dry, environments can be sterilized, the survival rate of the dormant eggs can be increased, and the purpose of storing large quantities of dormant eggs for a long term can be achieved.

Description

technical field [0001] The invention relates to the technical field of aquatic organisms, in particular to a freeze-drying and cold storage method for long-term preservation of resting eggs of Daphnia pacificus. technical background [0002] Copepods, belonging to the phylum Arthropoda, Crustacea, and Copepoda, are an important part of zooplankton. They are small crustaceans with a body length of <3mm. They engage in planktonic and parasitic life and are distributed in oceans, freshwater or brackish water. in the water. Because it is one of the opening baits for most fish larvae, it is of great significance in aquatic ecosystem food webs and aquaculture. Daphnia pacificis belongs to the order Daphnia, the family Daphnia, and the genus Daphnia. It is one of the coastal copepod species that is very common in low-salt areas. Daphnia pacificis mainly appear in summer and autumn, and start to lay dormant eggs at the end of autumn. The dormant eggs sink to the bottom of the s...

AI Technical Summary

Problems solved by technology

[0005] At present, there are still many difficulties in applying dormant eggs to the aquaculture industry. For example, the research on the preservation and hatching of dormant eggs is not enough, and it is impossible to control them according to the needs of large-scale production. Egg-germinated copepods are easy to prey on economic animal larvae, etc.

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