Freeze drying and refrigeration method for storing dormant eggs of eurytemora pacifica for long term

A technology for long-term storage and dormant eggs, which is applied in the field of aquatic organisms, can solve the problems of not being able to control it according to the needs of large-scale production, storage of dormant eggs, and insufficient hatching research, so as to increase the hatching germination rate, have repeatability, Reduce the effect of damage

Active Publication Date: 2019-01-04
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are still many difficulties in applying dormant eggs to the aquaculture industry. For example, the research on the preservation and hatching of dormant e

Method used

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  • Freeze drying and refrigeration method for storing dormant eggs of eurytemora pacifica for long term

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A protectant that contains Dimethyl Sulfoxide, Maleic Acid and Sodium Hyaluronate. The protective agent can maintain the integrity of the matrix in the dormant egg cells, and weaken the damage of low temperature to the tissue structure of the dormant eggs, so as to maintain the high activity of the dormant eggs and increase the hatching and germination rate of the dormant eggs.

[0022] The weight ratio of maleic acid in the protective agent is 0.056%, and the weight ratio of sodium hyaluronate to maleic acid is 1.3:0.6. When the dormant eggs are freeze-dried and refrigerated, the above-mentioned dormant egg protectant is added, in which maleic acid and sodium hyaluronate are interlinked by glycosidic bonds and unsaturated bonds, utilizing high cohesiveness and dimethyl sulfoxide The high permeability, enters the resting egg, and forms a certain osmotic pressure, so that matrix fibronectin is enriched on the plasma membrane outside the resting egg, and the rearrangement...

Embodiment 2

[0031] A protective agent, which comprises dimethyl sulfoxide, maleic acid and sodium hyaluronate, the weight ratio of maleic acid in the protective agent is 0.062%, and the weight ratio of sodium hyaluronate and maleic acid is 1.5: 0.6.

[0032] A freeze-drying cold storage method for long-term preservation of resting eggs of Daphnia pacificus, the specific steps are:

[0033] 1) The cleaning steps are as follows: place the dormant eggs in a 320-mesh sieve, wash off the culture medium on the surface of the eggs with double distilled water, and move them to a glass container filled with a protective agent;

[0034] 2) The nitrogen drying step is as follows: transfer the glass to the left and right transparent pipes, one end of the pipe is the air inlet, and the other end is the air outlet. After the liquid nitrogen is dried with an inert chemical desiccant, the circulating air pump passes through the air inlet. input, the input rate of dry nitrogen is 15L / min, and the nitrogen ...

Embodiment 3

[0037] A protective agent, which comprises dimethyl sulfoxide, maleic acid and sodium hyaluronate, the weight ratio of maleic acid in the protective agent is 0.074%, and the weight ratio of sodium hyaluronate and maleic acid is 1.4: 0.6.

[0038] A freeze-drying cold storage method for long-term preservation of dormant eggs of Daphnia pacificus, the specific steps are:

[0039] 1) The cleaning steps are as follows: place the dormant eggs in a 300-mesh sieve, wash off the culture medium on the surface of the eggs with double distilled water, and move them to a glass container containing a protective agent;

[0040] 2) The nitrogen drying step is as follows: transfer the glass to the left and right transparent pipes, one end of the pipe is the air inlet, and the other end is the air outlet. After the liquid nitrogen is dried with an inert chemical desiccant, the circulating air pump passes through the air inlet. input, the input rate of dry nitrogen is 25L / min, and the nitrogen...

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Abstract

The invention discloses a protective agent with dimethyl sulfoxide, maleic acid and sodium hyaluronate, and further discloses a freeze drying and refrigeration method for storing dormant eggs of eurytemora pacifica for a long term. The freeze drying and refrigeration method includes sieving and thoroughly cleaning the dormant eggs and then transferring the dormant eggs into a container with the protective agent; filling the container with circulating nitrogen dried by inert chemical drying agents; transferring the dormant eggs into light-resistant containers after the eggs are dried, and hermetically storing the dormant eggs. The freeze drying and refrigeration method has the advantages that the integrity of matrixes of the dormant eggs of the eurytemora pacifica can be kept by the protective agent, damage to tissue structures of the dormant eggs can be abated, the high activity of the dormant eggs can be kept, and the hatching rate of the dormant eggs can be increased; technologies ofthe freeze drying and refrigeration method for storing the dormant eggs of the eurytemora pacifica for the long term are easy to operate, the freeze drying and refrigeration method is repeatable, safe and reliable and is low in cost, systems are stable and dry, environments can be sterilized, the survival rate of the dormant eggs can be increased, and the purpose of storing large quantities of dormant eggs for a long term can be achieved.

Description

technical field [0001] The invention relates to the technical field of aquatic organisms, in particular to a freeze-drying and cold storage method for long-term preservation of resting eggs of Daphnia pacificus. technical background [0002] Copepods, belonging to the phylum Arthropoda, Crustacea, and Copepoda, are an important part of zooplankton. They are small crustaceans with a body length of <3mm. They engage in planktonic and parasitic life and are distributed in oceans, freshwater or brackish water. in the water. Because it is one of the opening baits for most fish larvae, it is of great significance in aquatic ecosystem food webs and aquaculture. Daphnia pacificis belongs to the order Daphnia, the family Daphnia, and the genus Daphnia. It is one of the coastal copepod species that is very common in low-salt areas. Daphnia pacificis mainly appear in summer and autumn, and start to lay dormant eggs at the end of autumn. The dormant eggs sink to the bottom of the s...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 叶莹莹曾善美黄友坤李继姬张建设
Owner ZHEJIANG OCEAN UNIV
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