Preparation method of hydrogel blended film embedded with quorum sensing inhibitor
A quorum sensing and inhibitor technology, which is applied in the field of preparation of a hydrogel blend membrane embedded with a quorum sensing inhibitor, can solve the problems of poor stability and easy inactivation, and achieves a simple and easy preparation method and a long service life. Long, improve the effect of low efficiency
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[0035] A method for preparing a blended film with embedded quorum sensing inhibitors includes the following steps:
[0036] 1) Preparation of the quorum sensing inhibitor solution: Weigh an appropriate amount of the quorum sensing inhibitor, dissolve it in deionized water, and stir at 10-30°C for 0.5-1.5 hours to prepare a 100-200 mg / ml quorum sensing inhibitor solution.
[0037] The quorum sensing inhibitors refer to all quenching enzymes that degrade bacterial signal molecules and bacteria containing quenching enzymes, including AHL acylase, lactase, decarboxylase, and deaminase of P.aeruginosa PAO1 , Bacillus thuringiensis and Rhodococcus BH4.
[0038] 2) Preparation of binary mixed solution: Dissolve sodium alginate and additives (a mixture of one or more of chitosan, carboxyl chitosan, sodium carboxymethyl cellulose) in deionized water according to the mass ratio , Stir, prepare 1~5g / L sodium alginate and additive solution; add 0.1~4wt% quorum sensing inhibitor solution account...
Embodiment 1
[0044] 1) Preparation of quorum sensing inhibitor solution: Weigh an appropriate amount of Bacillus thuringiensis, dissolve it in deionized water, and stir at 20°C for 1 hour to prepare a 200 mg / ml bacterial suspension.
[0045] 2) Preparation of binary mixed solution: Dissolve sodium alginate and chitosan in deionized water, stir and mix, prepare 1g / L sodium alginate and 2g / L chitosan solution, and mix 1wt% bacterial suspension Add into the sodium alginate mixed solution and stir; mix the sodium alginate solution mixed with Bacillus thuringiensis and the chitosan solution at a ratio of 6:1, stir at a uniform speed, and prepare a binary mixed solution.
[0046] 3) Preparation of gelatin solution: take a certain amount of gelatin to make a 5g / L solution, swell it at 10°C for 30 minutes for homogenization to obtain a gelatin solution.
[0047] 4) Preparation of the ternary mixed solution: The binary mixed solution prepared in step 2) and the gelatin solution prepared in step 1) are mix...
Embodiment 2
[0050] 1) Preparation of quorum sensing inhibitor solution: Weigh 0.1 mg of aminoacylase, dissolve it in deionized water, and stir for 1.5h at 10°C to prepare a 100mg / ml aminoacylase solution.
[0051] 2) Preparation of binary mixed solution: Dissolve sodium alginate and sodium carboxymethyl cellulose separately in deionized water, stir and mix to prepare 2g / L sodium alginate and 1g / L sodium carboxymethylcellulose solution, add 0.1 The wt% aminoacylase solution was added to the sodium alginate mixed solution and stirred. The sodium alginate solution mixed with aminoacylase and the sodium carboxymethyl cellulose solution are mixed at a ratio of 10:1, stirred at a uniform speed, and prepared to obtain a binary mixed solution.
[0052] 3) Preparation of gelatin solution: take a certain amount of gelatin to make a 1g / L solution, swell it at 30°C for 30 minutes for homogenization to obtain a gelatin solution.
[0053] 4) Preparation of the ternary mixed solution: The binary mixed solutio...
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