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Microbial culture medium and preparation method and application thereof

A microbial culture medium and micropowder technology, applied in the field of biopharmaceuticals, can solve the problems of high specificity of fermentation medium, application limitations, and limited application range, and achieve the effects of good metabolic cycle, wide application range, and wide applicability

Inactive Publication Date: 2019-01-08
SHANGHAI JINGFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] CN102732571A discloses a fermentation medium for producing calcitriol and a fermentation transformation method and application thereof. The fermentation medium includes a carbon source and a nitrogen source, wherein, based on the total volume of the fermentation medium, the amount of the carbon source The content of the nitrogen source is 5-80g / L; the content of the nitrogen source is 2-80g / L; the carbon source is selected from one or more of glucose, maltodextrin and soluble starch, and the nitrogen source is selected from yellow One or more of bean cake powder, cottonseed cake powder, peptone, yeast extract and yeast extract powder; this fermentation medium has high specificity and limited application range
This formula is only good for the fermentation production of tylosin, and its application is also relatively limited

Method used

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  • Microbial culture medium and preparation method and application thereof
  • Microbial culture medium and preparation method and application thereof
  • Microbial culture medium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The present embodiment provides a kind of microorganism culture medium, comprises the following components by weight part:

[0054]

[0055] Among them, the protein content of cottonseed cake powder is 45%, the protein content of peptone is 85%, and the protein content of yeast powder is 45%;

[0056] Preparation method: (1) Mix cottonseed cake powder, yeast powder, glucose, peptone, sodium dihydrogen phosphate, and disodium hydrogen phosphate in proportion at 40°C for 30 minutes to obtain a mixture;

[0057] (2) After dissolving acetyl-CoA, nitrate and Tween 80 in water to obtain a solution, inject the solution into the mixture of step (1) through three independent nozzles, and dry it by air flow at 60°C The micropowder with a fineness of 200 mesh is obtained, and the microbial culture medium is obtained after further mixing.

Embodiment 2

[0059] The present embodiment provides a kind of microorganism culture medium, comprises the following components by weight part:

[0060]

[0061] Among them, the protein content of cottonseed cake powder is 40%, the protein content of peptone is 80%, and the protein content of yeast powder is 40%;

[0062] Preparation method: (1) Mix cottonseed cake powder, yeast powder, glucose, peptone, sodium dihydrogen phosphate, and disodium hydrogen phosphate in proportion at 30°C for 10 minutes to obtain a mixture;

[0063] (2) After dissolving acetyl-CoA, lanthanum nitrate and Tween 80 in water to obtain a solution, inject the solution into the mixture of step (1) through three independent nozzles, and dry it by air flow at 50°C The micropowder with a fineness of 100 mesh is obtained, and the microbial culture medium is obtained after further mixing.

Embodiment 3

[0065] The present embodiment provides a kind of microorganism culture medium, comprises the following components by weight part:

[0066]

[0067] Among them, the protein content of cottonseed cake powder is 50%, the protein content of peptone is 90%, and the protein content of yeast powder is 50%;

[0068] Preparation method: (1) Mix cottonseed cake powder, yeast powder, glucose, peptone, sodium dihydrogen phosphate, and disodium hydrogen phosphate in proportion at 50°C for 60 minutes to obtain a mixture;

[0069] (2) After dissolving acetyl-CoA, rubidium nitrate and Tween 80 in water to obtain a solution, inject the solution into the mixture of step (1) through three independent nozzles, and dry it by air flow at 70°C The micropowder with a fineness of 200 mesh is obtained, and the microbial culture medium is obtained after further mixing.

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Abstract

The invention provides a microbial culture medium and a preparation method and application thereof. The microbial culture medium comprises the following components in parts by weight: 3.5-5.5 parts ofcottonseed meal, 0.8-1.4 parts of yeast powder, 4.5-8.5 parts of glucose, 4-5 parts of peptone, 0.05-0.1 part of sodium dihydrogen phosphate, 0.05-0.1 part of disodium hydrogen phosphate, 0.02-0.04 part of acetyl coenzyme A and 0.001-0.02 part of nitrate. By the provided microbial culture medium, absorption of microbes to nutrient substances and a related metabolic process can be promoted, stablegrowth and reproduction of the microbes are facilitated, and the microbial culture medium is excellent and is wide in applicability.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a microbial culture medium and its preparation method and application. Background technique [0002] my country's biopharmaceuticals are booming, and in the process of industrialization and large-scale production, the requirements for medium are getting higher and higher. The nutrient composition of the fermentation medium has an important influence on the growth, reproduction and synthesis rate of the strain. It not only enables the seeds to grow rapidly after inoculation to reach a certain mycelial concentration, but also enables the well-grown bacteria to rapidly synthesize the target product. Therefore, the composition of the fermentation medium is required to be reasonable, the nutrition is balanced, and there are also growth factors required for cell growth and specific elements, precursors and promoters required for product synthesis. The early stage of fermentation is th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16C12R1/19C12R1/865C12R1/84
CPCC12N1/16C12N1/18C12N1/20
Inventor 陈仁爱金修建沈琳罗璐
Owner SHANGHAI JINGFENG PHARMA
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