Method for determining inorganic arsenic in aquatic products

Abstract

The invention discloses a method for determining inorganic arsenic in aquatic products. The method is characterized in that an ammonium carbonate solution, of which the concentration is 50mmol / L and the PH is 9.5, is used as a reference condition for setting of a novel mobile phase, and before use, the solution is filtered through a 0.45-mum hydrophilic filter film and subjected to 8 minutes of ultrasonic degasification in an ultrasonic water bath at a flow rate of 1.0ml / min; and a column is flushed with ultrasonically treated water for 30 minutes and then flushed with the novel mobile phrasefor 8 minutes, a standard curve is created after the temperature reaches a certain value, determination is then carried out on a sample solution, 50 muL of the sample solution is sucked and injected into a liquid chromatograph and inductively coupled plasma mass spectrometer to obtain a chromatogram, and a total inorganic arsenic content is the sum of an arsenite content and an arsenate content inthe sample solution obtained according to the standard curve. The method disclosed by the invention has the advantages that proportioning of a mobile phase in the liquid chromatography and inductively coupled plasma mass spectrometry is greatly simplified, so that the time and operation difficulty in detecting the inorganic arsenic in the aquatic products are reduced, and the detection efficiencyis improved.

Description

technical field [0001] The invention relates to the technical field of inorganic arsenic detection, in particular to a method for determining inorganic arsenic in aquatic products. Background technique [0002] There are many forms of arsenic in aquatic products, mainly methyl arsinate and inorganic arsenic, among which inorganic arsenic is more toxic and carcinogenic. Aquatic products have a relatively strong ability to enrich arsenic. In aquatic products, arsenic mainly exists in the form of non-toxic or low-toxic organic arsenic, and the content of highly toxic inorganic arsenic is usually low. The detection of inorganic arsenic content in aquatic food is of great importance to guarantee The health and safety of consumers is of paramount importance. [0003] In the second part of GB 5009.11-2014 National Food Safety Standard, the specific method and detailed steps for the determination of inorganic arsenic in food are given. Among them, the second method is liquid chroma...

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Problems solved by technology

[0004] However, in the national standard liquid chromatography-inductively coupled plasma mass spectrometry (LC-ICP / MS), the mobile phase needs to be mixed with anhydrous sodium acetate, potassium nitrate, sodium dihydrogen phosphate, and disodium edetate to adjust the pH, and the configuration process is very cumbersome. , and the measurement requires a long retention time, it usually takes 20 minutes to produce all the peaks, resulting in long detection time and difficult operation

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