Hepatoprotective space Lactobacillus plantarum FH-SS18-86365L and application thereof

A technology of FH-SS18--86365L, Lactobacillus plantarum, applied in Lactobacillus, bacteria used in food preparation, application, etc., can solve the problem of reducing antioxidant capacity, increasing lipid peroxidation products, toxicity and safety To achieve the effect of protecting the liver, inhibiting the formation of fatty liver, and broadening the application prospects of the market

Active Publication Date: 2019-01-11
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, nutritional stress caused by a high-fat and high-sugar diet increases lipid peroxidation products and reduces antioxidant capacity
Synthetic Antioxidants Despite Promising Effects, Toxicity and Safety Questioned

Method used

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  • Hepatoprotective space Lactobacillus plantarum FH-SS18-86365L and application thereof
  • Hepatoprotective space Lactobacillus plantarum FH-SS18-86365L and application thereof
  • Hepatoprotective space Lactobacillus plantarum FH-SS18-86365L and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, the acquisition and preservation of Lactobacillus plantarum FH-SS18--86365L

[0034] Lactobacillus plantarum GS18 was subjected to space mutagenesis, and a large number of mutant strains were obtained after being carried back by the Shenzhou 10 and Shenzhou 11 spacecraft. From the mutant strains, a strain with a significant therapeutic effect on fatty liver was obtained. The strain was named Lactobacillus plantarum FH-SS18--86365L.

[0035] Lactobacillus plantarum FH-SS18--86365L, abbreviated as Lactobacillus plantarum SS18-86365L, full name Lactobacillus plantarum (Lactobacillus plantarum) FH-SS18--86365L, was preserved in China Microorganism Culture Collection Management on July 17, 2018 Committee General Microbiology Center (referred to as CGMCC; address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; Zip code: 100101), the deposit number is CGMCC No.16124.

Embodiment 2

[0036] Embodiment 2, the preparation of bacterial agent

[0037] 1. Inoculate the test bacteria preserved in the glycerol tube into 10mL liquid MRS medium with an inoculum amount of 2%, and culture it statically at 37°C for 16-20h.

[0038] 2. Inoculate the bacterial solution obtained in step 1 into 10 mL of liquid MRS medium with an inoculum amount of 2%, and culture it statically at 37°C for 16-20 hours.

[0039] 3. Inoculate the bacterial solution obtained in step 2 into 200 mL liquid MRS medium with an inoculum amount of 3%, and culture at 37° C. for 16-20 hours.

[0040] 4. Inoculate the bacterial solution obtained in step 3 into 5L liquid MRS culture medium with 3% inoculation amount, stir and ferment at 37°C and 120rpm for 16-20h (control pH7.0 during the fermentation process), and then centrifuge at 4°C and 6000rpm for 10min , to collect the bacterial pellet.

[0041] 5. Take all the bacterial precipitates obtained in step 4, add them to 500mL 15g / 100mL skim milk aqu...

Embodiment 3

[0049] Embodiment 3, the application of bacterial agent

[0050] 1. Preparation of bacterial solution

[0051] Dissolve every 1 g of the bacterium agent prepared in Example 2 in 0.85% sterile saline to obtain a diluted bacterium solution.

[0052] After diluting the SS18-33 bacterial agent, the bacterial concentration is 10 9 CFU / mL of SS18-33 bacterial liquid.

[0053] After diluting the SS18-119 bacterial agent, the bacterial concentration is 10 9 CFU / mL of SS18-119 bacterial liquid.

[0054] After diluting the SS18-86365L bacterial agent, the bacterial concentration is 10 9 CFU / mL of SS18-86365L bacterial liquid.

[0055] 2. Establishment of hyperlipidemia model in rats

[0056] Eight-week-old SPF male Wistar rats were divided into 4 groups (12 rats in each group), namely model group, SS18-33 group, SS18-119 group and SS18-86365L group. Rats in each group were operated as follows: they were first fed with SPF grade basal feed for 2 days, and then modeled. Modeling m...

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PUM

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Abstract

The invention discloses a hepatoprotectivespace Lactobacillus plantarum FH-SS18--86365L and an application thereof. The preservation number of the Lactobacillus plantarum FH-SS18--86365L is No. CGMCCNo. 16124. The invention also protects the plant Lactobacillus SS18-86365L is a bacterial agent of active ingredient. The invention also protects Lactobacillus plantarum SS18-86365L or that use of themicrobial agent in the preparation of the product. The use of the product is for the treatment and/or prevention of fatty liver. The use of the product is to treat and/or prevent hyperlipidemia. Theuse of the product is to protect the liver. The Lactobacillus plantarum SS18-86365L has that effect of protecting live and inhibiting the formation of fatty liver, and has wide market application prospect.

Description

technical field [0001] The invention relates to a space lactobacillus plantarum FH-SS18--86365L with hepatoprotective effect and application thereof. Background technique [0002] In recent years, with the improvement of living standards, people consume excess energy and lack of exercise, leading to a sharp increase in the risk of atherosclerosis, fatty liver, hyperglycemia and hypertension, which has become an increasingly serious global medical problem. [0003] Many studies have shown that the occurrence and development of fatty liver is closely related to oxidative stress. Oxidative stress is the overproduction of reactive oxygen species (ROS) and reactive nitrogen radicals (RNS) in the body when the body is subjected to harmful stimuli, resulting in an imbalance between the oxidative system and the antioxidant system. Excessive ROS produced can attack cellular proteins, lipids, and nucleic acids, leading to cellular dysfunction and inflammation. [0004] In addition, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/16A61P3/06A23L33/135A23C9/123C12R1/25
CPCA61P1/16A61P3/06A23C9/1234A23L33/135A61K35/747A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/30
Inventor 郝红炜刘慧张红星谢远红
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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