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Application of pterodontic acid in the preparation of medicine for preventing or treating influenza

A technology of malodox, medicine, applied in the field of medicine

Inactive Publication Date: 2019-01-15
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of this product on inhibiting influenza virus and down-regulating the inflammatory response mediated by influenza virus has not been reported

Method used

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  • Application of pterodontic acid in the preparation of medicine for preventing or treating influenza
  • Application of pterodontic acid in the preparation of medicine for preventing or treating influenza
  • Application of pterodontic acid in the preparation of medicine for preventing or treating influenza

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of malidan acid

[0022] Take 1 kg of dried medicinal material of stinky lingdan (collected from the suburbs of Kunming City), and extract 135 g of extract by percolation with 90% alcohol; add 800 ml of water to the extract and stir to suspend, then extract with petroleum ether, ethyl acetate, and n-butanol in sequence (5 × 500ml each), recover the solvent; mix the petroleum ether extract with an appropriate amount of silica gel, dry-pack the column (200-300 mesh, 650g), and use petroleum ether-ethyl acetate mixed solvent gradient elution under normal pressure. Solvent ratios are 1:0, 20:1, 10:1 and 5:1 in turn; receive and concentrate respectively; petroleum ether elution section weighs about 3.8g, continue to use silica gel column chromatography to separate repeatedly, alternately use petroleum ether and Chloroform was eluted, merged under the guidance of TLC (iodine fumigated color development), and concentrated to obtain a white crystal...

Embodiment 2

[0024] Embodiment 2: malamic acid inhibits the activity of influenza virus

[0025] 1. Materials

[0026]Cells: Dog kidney cells (Madin Darby Canine Kidney, MDCK) cells were cited from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.

[0027] Virus:

[0028] Influenza A virus PR8 strain (A / PR / 8 / 34, H1N1) and influenza A H3N2 influenza virus Aichi strain (A / Aichi / 2 / 68, H3N2) were purchased from the American Type Culture Collection (American Type Culture Collection, ATCC); the new type A H1N1 influenza virus strain (A / Guangzhou / GIRD07 / 09, H1N1, Genebank No.HM014332.1) is a clinical isolate in this laboratory, and the above-mentioned strain influenza virus was inoculated in 9-day-old chick embryo urine Amplify in the cystic cavity, incubate at 35°C for 2 days, and harvest the allantoic fluid. The virus was titrated with MDCK cells, and the half infectious dose (TCID50 / 100 μL) of these strains was determined by the cytopathic inhibition...

Embodiment 3

[0049] Embodiment 3: malamic acid inhibits influenza virus neuraminidase

[0050] 1. Materials and instruments

[0051] Reagents and medicines: malidan acid, self-made. Influenza Neuraminidase InhibitorResistance Detection Kit (U.S., Applied Biosystems company), chicken blood cells;

[0052] Instrument: HERAcell 150i constant temperature CO 2 Incubator (Thermo Company of the United States), DU800 ultraviolet and visible light spectrophotometer (Beckman Company of the United States)

[0053] Viruses and cells: new influenza A H1N1 strain (A / Guangzhou / GIRD07 / 09, H1N1, GenebankNo.HM014332.1), dog kidney cells (Madin Darby Canine Kidney, MDCK) cells were quoted from the Type Culture Collection Committee of the Chinese Academy of Sciences cell bank

[0054] 2. Experimental method

[0055] Use 0.5% chicken blood cells to measure the virus titer of the virus strain (reaching 16 hemagglutination units), then use 25 μl of the virus of 16 hemagglutination units and 25 μl of drugs...

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Abstract

The invention belongs to the field of medicine, and discloses a novel use of a sesquiterpene compound that is pterodontic acid in the preparation of anti-influenza drugs. For the first time, the inventors have discovered that pterodontic acid can significantly inhibit the replication of various subtypes of influenza viruses and effectively improve the inflammatory response caused by influenza viruses, thus revealing that pterodontic acid can be used for the prevention and treatment of influenza. The active ingredient comes from plants, the raw material is cheap and easy to obtain, has no obvious toxicity, and has great application prospect.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a medicine for preventing or treating influenza, and a new medical application of sesquiterpene, specifically, the application of malidanic acid in preparing a medicine for preventing and treating influenza. Background technique [0002] Influenza (abbreviated as flu) is an acute respiratory disease caused by influenza virus, which is highly contagious. Human influenza viruses are divided into three types: A, B, and C. Among them, influenza A is the most threatening to humans, and H1N1 is the most important seasonal influenza A virus. The global annual incidence of influenza is about 5%-10% in adults and 20%-30% in children, and it is easy to cause hospitalization and death in high-risk groups such as infants, the elderly or patients with chronic diseases. Influenza causes about 3 million to 5 million cases of severe disease and about 250,000 to 500,000 deaths every year in the world, seri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/19A61P31/16C07C57/26C07C51/42
CPCA61K31/19A61K36/28C07C51/42C07C57/26
Inventor 李菁王新华潘锡平杨子峰姜志宏关文达
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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