Carbon nanodot with fluorescence, photoacoustic and photothermal effects and synthesis method thereof

Abstract

Belonging to the field of material chemistry, the invention relates to a synthesis method of carbon nanodot with fluorescence, photoacoustic and photothermal effects. The method includes: using an FDAapproved safety excipient F-127, carrying out simple and convenient solid-phase synthesis reaction, removing the residual impurities of the reaction, and then conducting drying to obtain the carbon nanodot with fluorescence, photoacoustic and photothermal effects. The invention provides the new method for preparation of the carbon nanodot with fluorescence, photoacoustic and photothermal effects,and the prepared carbon nanodot has the characteristics of uniform size, stable and adjustable fluorescence emission, good photoacoustic-photothermal effect, high water dispersibility and good biocompatibility, and can be prepared into preparations widely applicable to photothermal therapy or combined therapy under the guidance of precise dual-mode imaging of tumors.

Description

technical field

[0001] The invention belongs to the field of material chemistry and relates to a carbon nano-dot and a preparation method thereof, in particular to a carbon nano-dot integrating fluorescence, photoacoustic and photothermal effects and a synthesis method thereof. Background technique

[0002] According to reports, precise imaging has very important guiding significance for the diagnosis and treatment of tumors, especially deep tumors. However, tumor imaging in current clinical practice is a single mode, which inevitably has its own limitations. For example, the defect in spatial resolution of highly sensitive fluorescence imaging may affect the judgment of tumor location, resulting in poor therapeutic effect. To reduce the extra toxic and side effects on surrounding normal tissues, integrating multi-mode imaging and complementing the advantages of different imaging modes to achieve real-time precise imaging has become a research hotspot at this stage. [0003...

Examples

Embodiment 1

[0038] The mixed solution of F-127, phenol, formalin and NaOH was reacted at 50°C for 17h, the reaction liquid was diluted, and heated in the reaction kettle (130°C) for 24h, the yellow precipitate was collected and washed with water to dry; Mix and stir the yellow powder with the catalyst, collect the dry powder, put it into a tube furnace, react at 350°C for 3 hours, ultrasonically disperse the obtained brown powder solution, centrifuge at high speed and dialyze to remove residual impurities, and freeze-dry to obtain a fluorescent, Carbon nanodots for photoacoustic and photothermal effects.

Embodiment 2

[0040] The mixed solution of F-127, phenol, formalin and NaOH was reacted at 66°C for 17h, the reaction liquid was diluted, and heated in the reaction kettle (130°C) for 24h, the yellow precipitate was collected and washed and dried; Mix and stir the yellow powder with the catalyst, collect the dry powder, put it into a tube furnace, react at 350°C for 3 hours, ultrasonically disperse the obtained brown powder solution, centrifuge at high speed and dialyze to remove residual impurities, and freeze-dry to obtain a fluorescent, Carbon nanodots for photoacoustic and photothermal effects.

Embodiment 3

[0042] The mixed solution of F-127, phenol, formalin and NaOH was reacted at 80°C for 17h, the reaction solution was diluted, and heated in the reaction kettle (130°C) for 24h, the yellow precipitate was collected and washed with water to dry; Mix and stir the yellow powder with the catalyst, collect the dry powder, put it into a tube furnace, react at 350°C for 3 hours, ultrasonically disperse the obtained brown powder solution, centrifuge at high speed and dialyze to remove residual impurities, and freeze-dry to obtain a fluorescent, Carbon nanodots for photoacoustic and photothermal effects.

technical field

[0001] The invention belongs to the field of material chemistry and relates to a carbon nano-dot and a preparation method thereof, in particular to a carbon nano-dot integrating fluorescence, photoacoustic and photothermal effects and a synthesis method thereof. Background technique

[0002] According to reports, precise imaging has very important guiding significance for the diagnosis and treatment of tumors, especially deep tumors. However, tumor imaging in current clinical practice is a single mode, which inevitably has its own limitations. For example, the defect in spatial resolution of highly sensitive fluorescence imaging may affect the judgment of tumor location, resulting in poor therapeutic effect. To reduce the extra toxic and side effects on surrounding normal tissues, integrating multi-mode imaging and complementing the advantages of different imaging modes to achieve real-time precise imaging has become a research hotspot at this stage. [0003...

Examples

Embodiment 1

[0038] The mixed solution of F-127, phenol, formalin and NaOH was reacted at 50°C for 17h, the reaction liquid was diluted, and heated in the reaction kettle (130°C) for 24h, the yellow precipitate was collected and washed with water to dry; Mix and stir the yellow powder with the catalyst, collect the dry powder, put it into a tube furnace, react at 350°C for 3 hours, ultrasonically disperse the obtained brown powder solution, centrifuge at high speed and dialyze to remove residual impurities, and freeze-dry to obtain a fluorescent, Carbon nanodots for photoacoustic and photothermal effects.

Embodiment 2

[0040] The mixed solution of F-127, phenol, formalin and NaOH was reacted at 66°C for 17h, the reaction liquid was diluted, and heated in the reaction kettle (130°C) for 24h, the yellow precipitate was collected and washed and dried; Mix and stir the yellow powder with the catalyst, collect the dry powder, put it into a tube furnace, react at 350°C for 3 hours, ultrasonically disperse the obtained brown powder solution, centrifuge at high speed and dialyze to remove residual impurities, and freeze-dry to obtain a fluorescent, Carbon nanodots for photoacoustic and photothermal effects.

Embodiment 3

[0042] The mixed solution of F-127, phenol, formalin and NaOH was reacted at 80°C for 17h, the reaction solution was diluted, and heated in the reaction kettle (130°C) for 24h, the yellow precipitate was collected and washed with water to dry; Mix and stir the yellow powder with the catalyst, collect the dry powder, put it into a tube furnace, react at 350°C for 3 hours, ultrasonically disperse the obtained brown powder solution, centrifuge at high speed and dialyze to remove residual impurities, and freeze-dry to obtain a fluorescent, Carbon nanodots for photoacoustic and photothermal effects.

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