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A sectional fermentation method for producing ganoderma lucidum

A technology of staged fermentation and Ganoderma lucidum fermentation, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2019-01-15
上海禾向健康科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although studies have shown that Ganoderma lucidum is a medicinal fungus with obvious pharmacological activity, which has the functions of enhancing immunity, lowering blood fat, lowering blood sugar, lowering blood pressure, protecting liver and liver, detoxifying, lowering cholesterol, and anti-tumor effects, but there is currently no A Ganoderma lucidum medicine with significant fat-lowering and blood-sugar-lowering functions

Method used

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  • A sectional fermentation method for producing ganoderma lucidum
  • A sectional fermentation method for producing ganoderma lucidum
  • A sectional fermentation method for producing ganoderma lucidum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1) Keep the temperature at 28.5±1.5°C, put PDA solid medium inoculated with Monascus HX01 into a 30L stirring tank, dilute it with water to form a liquid medium, place it on a shaker, and stir for 32±1h, control the stirring The speed is 350 rpm;

[0063] 2) Keep the temperature at 28.5±1.5°C, suck the liquid culture medium stirred in step 1) into a 300L seed tank under negative pressure, and cultivate for 52 hours;

[0064] 3) Keeping the temperature at 28.5±1.5°C, sucking the stirred liquid medium in step 2) into a 1.5-ton seed tank under negative pressure, and culturing for 52 hours;

[0065] 4) Keep the temperature at 25.5±0.5°C, put the PDA solid medium inoculated with Ganoderma lucidum No. 1 into a 30L stirring tank, dilute it with water to form a liquid medium, place it on a shaker, and stir it for 52 hours, controlling the stirring speed 300 rpm;

[0066] 5) Keep the temperature at 25.5±0.5°C, suck the liquid culture medium stirred in step 4) into a 300L seed ...

Embodiment 2

[0074] Except that the formulation of the coix seed acetic acid culture medium is different from that of Example 1, all the other operations are the same.

[0075] The results showed that the obtained hypoglycemic ganoderma lucidum contained 6.5% of ganoderma polysaccharide, 4.7% of ganoderma triterpenes, and 725mg / 1000g of lovastatin.

Embodiment 3

[0077] Except that the formulation of the coix seed acetic acid culture medium is different from that of Example 1, all the other operations are the same.

[0078] The results showed that the obtained hypoglycemic ganoderma contained 6.8% of ganoderma polysaccharide, 4.5% of ganoderma triterpenes, and 830mg / 1000g of lovastatin.

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Abstract

The invention provides a sectional fermentation method for producing ganoderma lucidum, which relates to the technical field of fungal fermentation. The method includes 1) culture of Monascus rubra ina shaking table to obtain Monascus seed liquid; 2) expand culture of Monascus seed liquid to obtain Monascus ferment species; 3) culture of Ganoderma lucidum in a shaking table to obtain Ganoderma lucidum seed solution; 4) enlarge culture of Ganoderma lucidum seed liquid to obtain Ganoderma lucidum ferment seed; 5) inoculation of Monascus fermenting specie into Coicis semen powder culture mediumto carry out first fermentation, then inoculation of Ganoderma lucidum fermenting specie, and second fermentation to obtain compound fermentation broth; 7) vacuum dry that solid initial product and pulverizing to obtain ganoderma lucidum. The ganoderma lucidum obtain by the method comprises 6-8 percent of ganoderma lucidum polysaccharide, 4-5 percent of ganoderma lucidum triterpenoids and 550-970mg / 1000g of lovastatin, which can remarkably enhance the lipid-lowering and sugar-lowering function of ganoderma lucidum.

Description

technical field [0001] The invention belongs to the technical field of fungal fermentation, and in particular relates to a staged fermentation method for producing ganoderma lucidum. Background technique [0002] Ganoderma lucidum (scientific name: Ganoderma Lucidum Karst), also known as Ganoderma lucidum grass, fairy grass, also known as red lucidum, red lucidum, wood ganoderma, mushroom ganoderma, ten thousand years mushroom, ganoderma grass, is the fruiting body of polyporaceae fungus ganoderma. The shape of Ganoderma lucidum is umbrella-shaped, and the cap is kidney-shaped, semi-circular or nearly circular. Ganoderma lucidum is a precious traditional Chinese medicinal material with a history of thousands of years of medicinal use. It has the effects of invigorating qi and calming the nerves, relieving cough and relieving asthma. Ganoderma lucidum has high medicinal value. After decades of modern pharmacological research by scientific research institutions, it has been c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 孙凯文孙怡凯岳晓天
Owner 上海禾向健康科技发展有限公司
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