High-efficiency genetic transformation method of whole plant infection method mediated by Agrobacterium alpina

Abstract

The invention discloses an efficient genetic transformation method of whole strain infection method mediated by Agrobacterium tumefaciens of Rhododendron alpinum. The method mainly comprises the following steps: seed pre-culture, ultrasonic treatment, preparation of Agrobacterium tumefaciens bacterial solution, agrobacterium infection and vacuum filtration, co-culture, degerming washing, germination culture, GUS histochemical staining. The method of the invention has the advantages of simple operation, low experiment cost, short transformation period and high genetic transformation efficiencyof 61%, and can be applied to the genetic transformation of other plants of Rhododendron.

Description

technical field [0001] The invention relates to the technical field of plant life sciences, in particular to a high-efficiency genetic transformation system invented by using Rhododendron delavayi, an alpine rhododendron, as an experimental material. Background technique [0002] Azalea (Rhododendron L.) is a world-renowned garden ornamental plant, one of the top ten traditional flowers in my country and one of the eight famous flowers in Yunnan Province, China. Alpine Rhododendron is a general term for some original species of evergreen broad-leaved Rhododendron and its hybrid offspring. Because of its beautiful plant shape, large and colorful flowers, and evergreen seasons, it has gradually become a rookie in my country's New Year's Eve flowers and high-end landscaping. There are more than 320 species of rhododendrons in my country, accounting for 1 / 3 of the world's rhododendrons. The southwest and adjacent eastern Himalayas are the distribution centers and origins of the ...

AI Technical Summary

Problems solved by technology

There are more than 320 species of rhododendrons in my country, accounting for 1 / 3 of the world's rhododendrons. The southwest and adjacent eastern Himalayas are the distribution centers and origins of the evergreen rhododendron subgenus, but the breeding research on rhododendrons started late. , lagging far behind the western countries, the Rhododendron species and their total quantity sold on the market in our country are still in the embarrassing situation of relying on foreign imports to a large extent

In this situation, in addition to conducting conventional cross-breeding research on Rhododendron plants, researchers must speed up their research on genetic improvement and breeding. However, the mature and efficient alpine Rhododendron genetic transformation technology still needs to be further developed

At present, the relevant research on the genetic transformation system of Rhododendron plants relies on tissue culture methods, and the research objects are limited to Azalea type Rhododendron plants. At the same time, there are bottleneck problems such as system instability and low transformation efficiency, which are difficult to solve.

The genetic transformation system of alpine Rhododendron species Rhododendron has not been successfully reported so far

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