Multi-beam multi-photon microscopy imaging device

Abstract

The invention provides a multi-beam multi-photon microscopic imaging device, comprising: a light source module, used to generate laser light that can be used for multi-photon excitation; a beam splitter, used to generate multiple beams distributed on a straight line with equiangular intervals laser; the resonant-galvanometer scanning head, the scanning direction of the resonant scanning head and the galvanometer scanning head are perpendicular to each other and the arrangement direction of the multi-beam laser is consistent with the scanning direction of the galvanometer scanning head; the focusing module is used to combine the resonant-vibration The laser light emitted by the mirror scanning head is converted into equidistant focused light spots to irradiate the sample to excite fluorescence or multi-photon higher-order harmonic signals; the imaging module is used to collect fluorescence or multi-photon higher-order harmonic signals for imaging. A multi-beam multi-photon microscopic imaging device provided by the present invention adopts multi-beam lasers distributed on a straight line, and the arrangement direction of the multi-beam lasers is consistent with the scanning direction of the galvanometer scanning head, so that the multi-beams can scan the sample at the same time Scanning, improved imaging speed.

Description

technical field [0001] The present invention relates to the field of optical technology, and more particularly, to a multi-beam multi-photon microscope imaging device. Background technique [0002] With the development of science and technology, optical microscopy imaging technology has become an important technology and measurement method in biology, medicine and related interdisciplinary research. Due to the characteristics of non-invasive, non-invasive, and rapid acquisition, optical microscopy imaging technology is very suitable for the imaging of living cells, biological tissues and non-biological systems, and has advantages that other technologies cannot replace. Therefore, in recent years, optical microscopy imaging technology has always been the frontier and hotspot of technological development, and new developments and breakthroughs have been made continuously. Its development trend is higher spatial resolution, faster speed, and dynamic biological information acqui...

AI Technical Summary

Problems solved by technology

[0003] A variety of focused illumination scanning imaging methods are provided in the prior art, but the imaging time resolution is low, and it is impossible to observe the biochemical dynamics in living cells and tissues at the millisecond and sub-millisecond levels, such as neuron activity and calcium signal. Fluctuation and excitation, molecular mechanism of neurotransmitter secretion, etc.

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