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A rapid detection method for illegally added prohibited drug fenfluramine in health food

A technology of illegally added and health food, applied in material analysis by observing the impact on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the lack of pertinence in health food analysis, the high toxicity of methylene chloride, etc. problem, to achieve the effect of simple operation, high sensitivity and obvious color rendering

Active Publication Date: 2021-10-29
广州智汇生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the detection objects of these methods are biological samples such as urine samples, blood samples or tissues, and pharmaceutical tablets, which are mainly used in forensic, toxicology, clinical and pharmaceutical fields, and lack certain pertinence for the analysis of health care products with complex matrices.
At present, 6 kinds of appetite suppressants, such as fenfluramine mixed in traditional Chinese medicine, have been analyzed and determined by HPCE analysis, but this method uses SPE mixed column solid phase extraction, and the eluent dichloromethane used is relatively toxic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] To detect whether the illegal drug fenfluramine is illegally added to a commercially available hard capsule health product, the specific method is as follows:

[0056] (1) Unscrew the capsule shell of the capsule health product, take 1.5g of powdered content and put it in a centrifuge tube, add 2ml of water and 0.8ml of hydrochloric acid solution with a molar concentration of 1mol / L, then shake it for 20s, then shake it at 3000rpm Centrifuge for 2 min at a high speed to obtain a centrifugate.

[0057] (2) Take 2ml of the centrifugate obtained in step (1), add 0.8ml of nitroaniline aqueous solution with a mass fraction of 1% and 1ml of sodium nitrate solution (obtained by dissolving 0.02g of sodium nitrate in 1ml of water), then shake gently for 10s; Continue to add 0.9ml of potassium hydroxide solution with a mass fraction of 2.5% and 1.6ml of n-butanol, shake for 30s, and then let it stand for 3min to make layers, and observe the color of the supernatant solution after...

Embodiment 2

[0060] To detect whether the illegal drug fenfluramine is illegally added to a commercially available soft capsule health product, the specific method is as follows:

[0061] (1) Cut open the soft capsule shell with scissors, squeeze out 2g of the liquid content and place it in a centrifuge tube, add 3ml of water and 1ml of hydrochloric acid solution with a molar concentration of 1mol / L, shake it for 30s, and then let it stand After 60s, the medicinal residues were precipitated to obtain a supernatant.

[0062] (2) Take 2ml of the supernatant obtained in step (1), add 1ml of 1% nitroaniline aqueous solution and 1.2ml of sodium nitrate solution (obtained by dissolving 0.02g of sodium nitrate in 1ml of water), then shake gently for 15s Continue to add 1ml of potassium hydroxide solution with a mass fraction of 2.5% and 2ml of n-butanol, shake for 40s, and then let stand for 4min to make layers, observe the color of the supernatant solution after layering.

[0063] Conclusion: T...

Embodiment 3

[0065] To detect whether the illegal drug fenfluramine is illegally added to a commercially available tablet health product, the specific method is as follows:

[0066] (3) Grind the tablet-shaped health product into powder, take 2.5g of the powdered content and put it in a centrifuge tube, add 4ml of water and 1.2ml of hydrochloric acid solution with a molar concentration of 1mol / L, shake it for 40s, and then put it in Centrifuge at a speed of 5000 rpm for 1.5 min to obtain a centrate.

[0067] (4) Take 2ml of the centrifugate obtained in step (1), add 1.2ml of 1% nitroaniline aqueous solution and 1.5ml of sodium nitrate solution (obtained by dissolving 0.02g of sodium nitrate in 1ml of water), then gently shake for 20s Continue to add 1.2ml of potassium hydroxide solution with a mass fraction of 2.5% and 2.4ml of n-butanol, shake for 60s, and then let stand for 5min to make layers, observe the color of the supernatant solution after layering.

[0068] Conclusion: The color ...

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Abstract

The invention discloses a rapid detection method for illegally added prohibited drug fenfluramine in health food, comprising: (1) taking a sample to be tested, adding water and reagent A, shaking, centrifuging or standing still; (2) taking Add reagent B and reagent C to the centrifuge or the supernatant after standing still, shake gently, continue to add reagent D and reagent E, shake, stand still, observe the color of the upper layer solution after standing and stratifying, reagent A is hydrochloric acid solution, sulfuric acid solution and glacial acetic acid, reagent B is p-nitroaniline aqueous solution, reagent C is one or both of sodium nitrite solution and potassium nitrite solution, reagent D is sodium hydroxide , potassium hydroxide, sodium carbonate and potassium carbonate, and reagent E is one or more of n-butanol, chloroform, ether and petroleum ether. The detection method of the invention is more environmentally friendly, faster and more accurate, has obvious color development, high sensitivity, simple operation, no need for any large-scale equipment, and wide adaptability.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a rapid detection method for illegally adding the prohibited drug fenfluramine in health food. Background technique [0002] Fenfluramine is also known as fenfluramine, fenfluramine hydrochloride, fenfluramine hydrochloride, Andre slimming pills or phenmeline tablets, etc. Its molecular formula is C 12 h 1 6F3N, molecular weight 231.25700, boiling point: 243.1°Cat, 760mmHg. Fenfluramine is an amphetamine-type appetite suppressant, which has a weak excitatory center effect and can lower blood pressure; it can also strengthen the utilization of glucose by peripheral tissues to lower blood sugar; it can also lower cholesterol, triacylglycerol and total plasma lipids. qualitative role. It is clinically used for simple obesity and obese patients with diabetes, hypertension, anxiety and cardiovascular disease; it also has a certain effect on the treatment of autism. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 李丰毓志超郝燕娟陈仙月
Owner 广州智汇生物科技有限公司
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