Improved magnetic silicon particles and method for nucleic acid purification thereof

A silicon particle and magnetic technology, which is applied in the field of improving magnetic silicon particles and its use in nucleic acid purification, can solve the problems of library construction influence, large specific surface area, false height, etc.

Active Publication Date: 2019-01-15
SUZHOU ENRICHING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This puts forward higher requirements for magnetic bead nucleic acid extraction, and there are still some downstream applications such as whole exome sequencing, gene chip, Southern blotting technology, fluorescent in situ hybridization technology, etc. still using columns Nucleic acid extraction by the column method is because the quality of the nucleic acid extracted by the column method is relatively stable, 260/230 is above 1.8, and 260/280 is in the range of 1.7 to 1.9. A problem with the magnetic bead method is that the ultraviolet spectrophotometer measures The concentration is quite different from t...

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  • Improved magnetic silicon particles and method for nucleic acid purification thereof
  • Improved magnetic silicon particles and method for nucleic acid purification thereof

Examples

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Embodiment 1

[0051] A preparation method of two-dimensional titanium dioxide nanosheets is provided:

[0052] 30g of n-butyl titanate, 25g of DMF, 30g of isopropanol, and 12g of octadecylamine were stirred magnetically for 1 hour, heated to 300 degrees after nitrogen gas was introduced, and continued to react for 5 hours. After the precipitate was washed, it was placed in isopropanol and configured to a mass fraction of 5%. Add 12g of isopropyl titanate, 3g of orthosilicate methyl ester, 2g of concentrated ammonia water and 20g of deionized water to the isopropanol of the above-mentioned 100g of titanium dioxide coarse flakes, mechanically stir at 300rpm, react at 50°C for 12 hours, and centrifuge at 2000rpm Wash with ethanol and deionized water to obtain hydrophilic modified two-dimensional titanium dioxide nanosheets with a thickness of 30nm and a particle size of 10-20um.

Embodiment 2

[0054] A preparation method (co-precipitation method) of a two-dimensional magnetic titanium dioxide sheet is provided:

[0055] Take 10g of the two-dimensional titanium dioxide nanosheets from Example 1 and disperse them in deionized water, add 2g of ferrous chloride tetrahydrate and 4.5g of ferric chloride hexahydrate and 200mL of deoxygenated deionized water, and mechanically stir for 30 Minutes later, 20mL of 1M sodium hydroxide solution was added dropwise, the temperature was raised to 80°C and the reaction was continued for 30 minutes, the temperature was lowered to room temperature, and after magnetic separation, it was washed with deionized water and dried at 60°C to obtain a two-dimensional magnetic titanium dioxide sheet.

Embodiment 3

[0057] Provide another preparation method (hydrothermal method) of two-dimensional magnetic titanium dioxide sheet:

[0058] Take 10g of dry two-dimensional titanium dioxide nanosheets from Example 1 and disperse them in 200mL of ethylene glycol, add 13g of sodium acetate, 12.4g of ferric chloride hexahydrate and 3.6g of sodium citrate, and stir mechanically for 1 hour. Put it into a high-pressure reactor and react at 180 degrees for 8 hours, wash it with deionized water after magnetic separation, and obtain a two-dimensional magnetic titanium dioxide sheet.

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Abstract

The invention discloses an improved magnetic silicon particle. The preparation method of the improved magnetic silicon particle comprises the following steps: 1) preparing two-dimensional titanium dioxide nano-sheet; 2) prepare a two-dimensional magnetic titanium dioxide sheet; 3) prepare improved silicon-coated two-dimensional magnetic titanium dioxide wafer. The improved magnetic silicon particles of the invention have high binding capacity to nucleic acids and little adsorption to salt ions, proteins or other impurities, and can effectively solve the phenomenon of virtual high nucleic acidextraction concentration. A method for improve magnetic silicon particles for nucleic acid purification include such steps as using improved magnetic silicon particles as magnetic particle for nucleicacid purification, preparing nucleic acid purification kit with lysis solution, binding solution, cleaning solution and eluent, and extracting high-purity and high-yield nucleic acid from biologicalsample through that kit.

Description

technical field [0001] The invention relates to the field of bionano materials, in particular to an improved magnetic silicon particle and a method for purifying nucleic acid. Background technique [0002] Conventional methods for extracting nucleic acids from biological samples involve guanidinium isothiocyanate-phenol-chloroform extraction, alcohol precipitation and column separation techniques. However, these processes are time-consuming and difficult to automate. Since Vogelstein et al. reported that glass powder was used as an adsorbent for extracting DNA fragments from agarose gels in the presence of high concentrations of sodium iodide, solid-phase nucleic acid purification techniques based on silica gel and other carriers with hydrophilic surfaces have been widely used. The nucleic acid purification method based on magnetic particles is one of them. Nucleic acid purification using magnetic separation technology usually goes through three steps: adsorption, washing,...

Claims

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Application Information

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IPC IPC(8): H01F41/00H01F1/00C12N15/10
CPCC12N15/1013H01F1/009H01F41/00
Inventor 张佳斌刘栋然曲峰尚春庆
Owner SUZHOU ENRICHING BIOTECH CO LTD
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