Papaya tissue culture seedling rooting and transplanting method

Abstract

The invention belongs to a papaya tissue culture seedling rooting and transplanting method, which has the steps of using papaya field matured lateral buds as explants; after disinfection and sterilization, planting the explants into a culture medium for primary induction culture and subculture rejuvenation culture to obtain adventitious buds; cutting down the adventitious buds from the base part;transplanting the adventitious buds to a mixed culture medium containing improved 1 / 2MS+0.8mg / LIBA+20g / L saccharose and vermiculite according to a ratio of 1:1 for rooting culture to obtain young seedlings; after 15 days, putting the obtained young seedlings at the normal temperature environment; performing seedling domestication for 2 to 3 days at the normal temperature environment; cleanly washing vermiculite; eliminating leaf-free rootless and damage seedlings; then, performing transplanting. The improved 1 / 2MS culture medium is a condensing agent culture liquid with halved major elements and no carrageenan / agar, wherein the monopotassium phosphate content in the major elements is improved from the conventional 170mg / L to 270mg / L. The rooting rate of the papaya tissue culture seedlingscan be improved; the generation of inflated monstrous spongy roots is reduced; the rooting culture link is simplified; the transplanting survival rate is improved.

Description

technical field [0001] The invention relates to a method for rooting and transplanting papaya tissue-cultured seedlings, and specifically relates to taking papaya tissue-cultured sub-generation rejuvenated seedlings as the target object, and then promoting their rooting to form a complete plant, increasing seedling growth through hardening and transplanting. The method for plant survival rate belongs to the field of biotechnology. Background technique [0002] Many scientific research workers at home and abroad have made some progress and done a lot of useful work in aspects such as papaya breeding and tissue culture. The tissue culture technology has also been reported, but it has not been used on a large scale to replace seedlings in actual production. Many key restrictive factors, among which papaya tissue culture seedlings are difficult to grow normal roots, and the low survival rate of transplanting is one of them. In recent years, there have been some related domestic...

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Problems solved by technology

The rooting rate of this rooting method has been greatly improved, but there are still some deformed and enlarged roots, and there are few normal roots with fine and dense root hairs, which affect the survival rate of transplanting.

[0005] Master thesis "Research on Papaya Tissue Culture and Rapid Propagation Technology System" published by Wang Longfu in 2010 (Wang Longfu, Research on Papaya Tissue Culture and Rapid Propagation Technology System [D]. Zhangjiang: Guangzhou Ocean University, Master Thesis, 2010.1- 53.) Three kinds of "one-step rooting method", "two-step rooting method" and "cutting rooting method" were proposed, among which "one-step rooting" and "two-step rooting" are similar to Zhou Peng, Chen Hanxin, etc. mentioned earlier, all using The traditional tissue culture medium formula MS+carrageenan / agar+hormone is used for rooting, and the roots produced all have enlarged and deformed spongy abnormal root systems. Compared with "one-step rooting", the number of deformed and enlarged roots in "two-step rooting" is However, the two-step rooting process is relatively cumbersome. It is first inoculated in MS medium for 3 days and then transferred to another MS medium. Frequent transfers will not only cause damage to the plants, but also increase the pollution rate and increase the cost, which is very unfavorable. Large-scale production in the future; the proposal of the cutting rooting method provides a new idea for the traditional rooting culture. The rootless seedlings are soaked in the mixture of root-inducing agent and fungicide and then inserted into the matrix. This method saves time. Labor-saving, but the cutting method has a very high mortality rate, and the optimal solution given in the paper has a mortality rate of more than 50%, which cannot meet the needs of large-scale production in the future

[0006] "Papaya 'Tainong Hybrid No. 2' Tissue Culture Technique" published by Zhang Xiuchun et al. in 2016 (Zhang Xiuchun, Zhao Pingjuan, Xian Shuli, Liu Zhixin. Papaya 'Tainong Hybrid No. 2' Tissue Culture Technique[J]. Molecular Plant Breeding , 2016,14(12): 3479-3482.) Proposed a rooting method similar to Wang Longfu's "two-step rooting method", and obtained normal rooted plants through two transfers. The difference from the former is that the culture medium for the second transfer has changed , adding vermiculite on the basis of the traditional culture medium, which is also an improvement on the basis of the traditional culture medium in recent years. damage, increasing the pollution rate and increasing the cost, which is very unfavorable for the old problem of large-scale production in the future

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