Fish gelatin/sodium alginate double-network complex hydrogel, preparation method thereof and obtained probiotic microcapsule

A technology of composite hydrogel and sodium alginate, which is applied in the direction of fixing on/in the organic carrier, can solve the problems of poor mechanical properties and stability, weak protection of probiotics, and unfavorable encapsulation of probiotics. Achieve the effect of high survival rate of probiotics, high gel strength and high water content

Inactive Publication Date: 2019-01-18
SHANDONG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] However, when fish gelatin gel and sodium alginate gel are used as single-network gels, they have the disadvantages of poor mechanical properties and stability, which is not conducive to the embedding of probiotics, resulting in a low encapsulation rate of probiotics, which is harmful to the protection of probiotics. little protection

Method used

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  • Fish gelatin/sodium alginate double-network complex hydrogel, preparation method thereof and obtained probiotic microcapsule
  • Fish gelatin/sodium alginate double-network complex hydrogel, preparation method thereof and obtained probiotic microcapsule
  • Fish gelatin/sodium alginate double-network complex hydrogel, preparation method thereof and obtained probiotic microcapsule

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preparation example Construction

[0033] The embodiment of the present invention provides a preparation method of fish gelatin / sodium alginate double network composite hydrogel, comprising the following steps:

[0034] S1: Prepare sodium alginate solution and fully hydrate it. In this step, the sodium alginate solution is prepared, and the sodium alginate solution is refrigerated at 4° C. for 24 hours to fully dissolve and hydrate it.

[0035] S2: adding fish gelatin to the above-mentioned hydrated sodium alginate solution, then subjecting the mixed solution to heat-induced treatment and cooling to obtain a gel mixed solution. In this step, fish gelatin has low-temperature self-gelation and thermal reversibility. When the temperature of fish gelatin denatured by heating drops below the critical temperature of gelation, the fish gelatin molecules transform from random coils to triple helical structures, thereby forming The three-dimensional network structure of the gel.

[0036] S3: adding the above-mentioned...

Embodiment 1

[0052] Weigh a certain amount of sodium alginate powder, prepare a sodium alginate solution with a mass concentration of 0.015 g / mL with distilled water, stir magnetically for 1 hour to fully dissolve the sodium alginate, and place it in a 4°C refrigerator for 24 hours to fully hydrate it. Weigh a certain amount of fish gelatin powder and add it to the hydrated sodium alginate solution, heat to 60°C with a magnetic stirrer, stir for 30 minutes, and cool to obtain a gel mixture, in which the concentration of fish gelatin is 0.01g / mL. Use a 1mL syringe (needle diameter 0.45 mm) to drop the gel mixture into 0.15 mol / L calcium chloride solution at a constant speed to form, and then place the formed gel beads in 0.35 mol / L calcium chloride solution to harden for 1 hour. Fish gelatin / sodium alginate double network composite hydrogel was obtained.

Embodiment 2

[0054] The preparation method of the fish gelatin / sodium alginate double network composite hydrogel is the same as in Example 1, except that the concentration of the fish gelatin is 0.02 g / mL.

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Abstract

The invention discloses fish gelatin/sodium alginate double-network complex hydrogel, a preparation method thereof and an obtained probiotic microcapsule, and belongs to the field of microcapsules. When the fish gelatin and sodium alginate gel as single network gel is used, the disadvantages of poor mechanical performance and stability are shown, and embedding of probiotics is not facilitated so as to cause the problems of low embedding ratio of the probiotics and small protection degree of the probiotics. According to the technical scheme, a sodium alginate solution is prepared and is fully hydrated; the fish gelatin is added into the hydrated sodium alginate solution to obtain a mixed solution; the mixed solution is heated, subjected to induction treatment, and is cooled to obtain a gelmixed solution; the gel mixed solution is added into a first calcium ion solution at constant speed and is molded, and then the molded gel is added into a second calcium ion solution and is hardened.The fish gelatin/sodium alginate double-network complex hydrogel disclosed by the invention can be applied to embedding of the probiotics and has the advantages of high embedding ratio and high survival rate of the probiotics.

Description

technical field [0001] The invention belongs to the field of microcapsules, and in particular relates to a fish gelatin / sodium alginate double network composite hydrogel, a preparation method thereof and the obtained probiotic microcapsules. Background technique [0002] As a medium of health transmission, probiotics have many beneficial effects on human health, such as providing lactic acid to the human body, decomposing indigestible and decomposed substances, and protecting against the infection of harmful pathogens. However, the activity of probiotics in food is easily affected by heat treatment, mechanical processing, physical state of food matrix, chemical microenvironment, storage conditions and other factors. The effect will destroy most of the probiotics. Therefore, maintaining the viability of probiotics is the key to its development and application. [0003] At present, microencapsulation technology is one of the most practical and effective means to protect prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C12N11/10C12N11/02C08L89/00C08L5/04
CPCC08J3/075C08J2389/00C08J2405/04C12N11/02C12N11/10
Inventor 陈浩刘佳林刘凤华
Owner SHANDONG UNIV
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