Respiratory bottle for measuring respiratory rate of biofilm, measuring device and measuring method

Abstract

The invention discloses a breathing bottle for measuring the breathing rate of a biofilm, comprising a bottle body, a carbon dioxide absorbing tube and a perforated separator arranged inside the bottle body; a perforated partition plate divides the inside of the bottle body into an oxygen mass transfer stirring zone provided with a stirring device and a biofilm reaction zone used for placing MBBRfillers; the carbon dioxide absorbing tube is provided with a permeable hole and contains a solution capable of absorbing CO<2>; a perforated separator has a first hole corresponding to a middle portion and a lower portion of the perforated separator and serving as a liquid exchange channel and a second hole corresponding to an upper portion of the perforated separator and serving as a gas exchange channel, and the oxygen transfer stirring zone is communicated with the biofilm reaction zone through the liquid exchange channel and the gas exchange channel. On the one hand, the fact that gas-liquid exchange of the oxygen transfer stirring zone and the biofilm reaction zone is fully processed is ensured. On the other hand, the stirring device can avoid the destruction of biofilm on MBBR packing, so the respiratory kinetic characteristics of biofilm on MBBR packing can be measured in situ. The determination process is simpler, quicker, and the determination results are more accurate and effective.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a breathing bottle, a measuring device and a measuring method for measuring the respiration rate of a biofilm. Background technique [0002] Fluidized bed biofilm reactor (MBBR) is a new type of biofilm reactor, which is an improved new composite biofilm developed on the basis of fixed bed reactor, fluidized bed reactor and biofilter. reactor. The process requires the use of suspended fillers to provide a suitable growth environment for microorganisms. Determining the respiratory dynamics characteristics of biofilm on MBBR suspended packing is extremely important for optimizing the design and operation of sewage treatment process. [0003] There are two existing methods for measuring the respiration dynamics of biofilms on MBBR fillers. One is to directly put the filler into the breathing bottle, and match it with magnetic stirring, so as to conduct a breath dynamics test; duri...

AI Technical Summary

Problems solved by technology

One is to directly put the filler into the breathing bottle, and match it with magnetic stirring, so as to conduct a breath dynamics test; during the test, the filler will interfere with the rotation of the magnetic stirrer and affect the oxygen mass transfer; in addition, the magnetic stirrer will put the filler on the The biofilm is scraped off, thereby destroying the biofilm structure and affecting the measurement results; in fact, the measurement cannot be realized

The other is to directly centrifuge the MBBR filler to make the biofilm on the filler fall off, suspend it in water, and then carry out the respiratory dynamics experiment according to the conventional method; this method has caused irreversible damage to the biofilm on the filler; At the same time, the shedding of the biofilm leads to changes in the mass transfer rate of oxygen on the surface of the filler biofilm, which cannot truly reflect the respiratory dynamics of the biofilm.

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