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Sewage treatment bacterial product using oyster shell as carrier

A technology for sewage treatment and oyster shells, applied in the field of environmental treatment, can solve problems such as inhibiting the growth of ammonia nitrogen-reducing bacteria, and achieve the effect of solving the low efficiency of outdoor sewage treatment

Active Publication Date: 2019-01-25
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, in addition to the high ammonia nitrogen content in domestic sewage, the sludge produced after sewage treatment also contains toxic and harmful substances, such as toxic heavy metal ions and other substances; thus inhibiting the growth of ammonia nitrogen-reducing bacteria

Method used

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  • Sewage treatment bacterial product using oyster shell as carrier

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Screening and identification of strains

[0022] 1. Preliminary screening

[0023] In January 2016, samples of sewage and bottom sludge in the sewage channel were collected in Liuting Industrial Park, Qingdao. The water temperature was 5°C when sampling; Nutrient-polluted water bodies with ions seriously exceeding the standard. After filtering the retrieved sample, the filtrate was carried out with purified water for 10 1 -10 4 After doubling dilution, 10 μl of the diluted solution was spread on LB solid medium for cultivation at a temperature of 14°C, and colonies visible to the naked eye appeared after 72 hours of cultivation. Then the colonies visible to the naked eye were streaked onto the heavy metal-resistant directional screening culture plate and cultivated (10 g of peptone, 5 g of yeast, 10 g of NaCl, H 2 O; Pb 2mg / L, Cu 2mg / L; the concentrations of Pb and Cu are 20 times that of the nationally specified Class V water); the cultivation tempera...

Embodiment 2

[0033] Embodiment 2: qd-wb-n5 strain is to the treatment effect of sewage

[0034] After the qd-wb-n5 strain was expanded with LB medium, it was inoculated into the sewage water body collected by the sewage treatment plant according to the inoculum amount of 10%. The Ph value of the water body was 6.8, and the COD value was 695.2mg / L. The concentration of heavy metal Pb is 1.13mg / L. Cultivate at 14°C and 35°C in a constant temperature water bath at 150rpm / min for 20h, then centrifuge at 3000rpm / min for 10min, take the supernatant, and measure the COD value of the supernatant. At the same time, the Bacillus subtilis CM-A12 strain preserved in the China Center for Type Culture Collection was used as a control; three parallel experiments were carried out for each strain.

[0035] The results showed that the organic matter degradation rate of qd-wb-n5 strain was 47.94% at 14℃; while the organic matter degradation rate of CM-A12 strain was only 23.40% under the same conditions. U...

Embodiment 3

[0039] Embodiment 3 prepares biofilm with oyster shell as carrier

[0040]After expanding the qd-wb-n5 strain, the oyster shell was used as a carrier to prepare biofilm.

[0041] 1) Expansion culture of bacterial strains: inoculate qd-wb-n1 bacteria in the expansion medium to obtain expansion culture medium; expansion medium is to add sodium chloride 8-12g, tryptone 7-13g, yeast powder 4 to 1000ml -7g; adjust pH 7.0-7.8;

[0042] The conditions for expanded culture are as follows: 20-25°C, 100-120r / min water bath shaker culture for 24-32h;

[0043] 2) Bacteria adhere to the oyster shell powder: put the pulverized oyster shell into a mesh bag after being sterilized, and put it into a container for hanging film; then evenly sprinkle the nutrient solution of step 1) on the oyster shell, Then add a glucose solution with a concentration of 1.0-2.0g / L in the container to promote the growth of microorganisms; after culturing for 5-7 days, a biofilm will be formed; mucus will appear...

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Abstract

The invention provides a sewage treatment product, namely a sewage treatment bacterial product using an oyster shell as a carrier. The sewage treatment bacterial product provided by the invention is prepared by inoculating bacterial solution of sewage treatment bacteria on oyster shell for culture; wherein the sewage treatment bacteria are Bacillus qd-wb-n5 strain deposited on July 2, 2018 at theChinese Typical Culture Collection Center of Wuhan University, Wuhan, China, deposit number CCTCC M 2018432. The invention uses the screened Bacillus qd-wb-n5 strain, the biofilm made of oyster shellcan effectively treat sewage and sludge formed by sewage under low temperature, so as to effectively solve the problem of low efficiency of outdoor sewage treatment in northern winter.

Description

technical field [0001] The invention belongs to the technical field of environmental treatment, and in particular relates to a sewage treatment fungus product using oyster shells as a carrier. Background technique [0002] Urban sewage refers to domestic sewage, industrial wastewater and runoff sewage within urban areas. Generally, it is collected by urban pipes and canals and should be treated by urban sewage treatment plants before being discharged into water bodies. In addition to containing a large amount of organic matter, germs and viruses in urban sewage, due to the high development of industry, the water volume of industrial wastewater (accounting for about 60-80% of the total urban sewage), the water quality is becoming more and more complex and the pollution of runoff sewage is becoming more and more serious, making urban sewage Contains various types and degrees of various toxic and harmful pollutants. The treatment of urban sewage involves many aspects, and com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/02C02F3/34C12R1/07
CPCC02F3/34C12N1/20C12N11/02
Inventor 秦志华郭沛张林林张慧单虎
Owner QINGDAO AGRI UNIV
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