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Cancer marker and use thereof

A marker and cancer technology, applied in the field of tumor molecular biology, can solve the problems of low sensitivity and specificity, overtreatment, etc., and achieve the effect of improving the treatment effect

Active Publication Date: 2019-01-25
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is that the existing molecular markers for diagnosing prostate cancer have the defects of low sensitivity and specificity, and are prone to overtreatment, so as to provide a prostate cancer-related marker with high specificity and sensitivity. Molecular markers of

Method used

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  • Cancer marker and use thereof
  • Cancer marker and use thereof
  • Cancer marker and use thereof

Examples

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Embodiment 1

[0068] The present embodiment provides a method for detecting the protein expression level of APMAP gene in prostate cancer tissue (Tumor) and normal tissue (Normal), the specific content is as follows:

[0069] 1. Test samples

[0070] Prostate cancer tissue microarray chip (purchased from Xi'an Alina Biotechnology Co., Ltd.), including 80 cases of prostate cancer, 8 cases of marginal normal prostate tissue and 8 cases of normal prostate tissue, each tissue sample was selected 2 detection points.

[0071] 2. Use the UltraSensitiveTMS-P method of immunohistochemistry (IHC) to detect the expression of APMAP gene in prostate cancer tissue (Tumor) and normal tissue (Normal, including marginal normal tissue and normal prostate tissue). The experimental steps of IHC include:

[0072] 1) Baked the slices at 60°C for 30 minutes, and dewaxed and hydrated conventionally;

[0073] 2) Antigen retrieval: Tris-EDTA (PH8.0) microwave antigen retrieval, cooled to room temperature, Tris (Tri...

Embodiment 2

[0098] The present embodiment provides a method for detecting the mRNA expression level of APMAP gene in prostate cancer tissue (Tumor) and normal tissue (Normal) by using bioinformatics analysis, and the specific content is as follows:

[0099] Obtain the mRNA expression of APMAP in prostate cancer tissue samples and adjacent normal tissues. The expression data of APMAP and patient samples are downloaded from TCGA (https: / / cancergenome.nih.gov / ), and APMAP is detected by Wilcoxonrank sum test. Differences in expression levels between normal and cancerous tissues.

[0100] image 3 Show APMAP gene in prostate cancer tissue (Cancer) and normal tissue (Normal) mRNA expression differential level, by image 3 It can be seen that the expression level of APMAP gene is significantly higher than that in normal tissues (p<0.001) no matter in paired or unpaired prostate cancer tissues. It was further verified that the expression of the APMAP gene is related to prostate cancer, and the...

Embodiment 3

[0102] This embodiment provides a method for evaluating the diagnostic value of APMAP gene expression level for prostate cancer. The specific content is: the prostate cancer patient data obtained through the TCGA database in Example 2 is edited by software to obtain the ROC (receiver operator characteristic curve )curve.

[0103] The drawing of ROC curve can use software or system of prior art field, such as=MedCalc9.2.0.1 medical statistical software, SPSS 9.0, ROCPOWER.SAS, DESIGNROC.FOR, MULTIREADER_POWER.SAS, R-studio, CREATE-ROC.SAS >GB STATV10.0 (Dynamic Microsystems, Inc. Silver Spring, MD, USA) and so on. The software used to draw the ROC curve in this embodiment is R-studio.

[0104] The ROC curve is a comprehensive index that reflects the continuous variables of the true positive rate (sensitivity, also known as sensitivity) and false positive rate (specificity, specificity), and uses the composition method to reveal the relationship between sensitivity and specific...

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Abstract

The invention discloses a cancer marker, wherein the cancer is a prostate cancer, and the cancer marker is an expression product of an APMAP gene and / or an APMAP gene. The APMAP gene is highly expressed in prostate cancer, which can be used for early diagnosis of prostate cancer, and has the advantages of specificity and high sensitivity. The high expression of APMAP gene is closely related to thepoor prognosis, distant metastasis and lymph node metastasis of prostate cancer, suggesting that APMAP gene can be used as a prognostic marker to provide effective information for the metastasis diagnosis, prognosis evaluation and treatment effect monitoring of prostate cancer. The invention discloses an expression inhibitor of an APMAP gene, comprising siRNA and / or shRNA, which has good interference effect on the expression of the APMAP gene, and has the application potential of clinical gene therapy. The invention discloses a cell strain of stable knockdown APMAP gene, which is favorable for realizing further research on gene function.

Description

technical field [0001] The invention relates to the field of tumor molecular biology, in particular to a cancer marker, application, expression inhibitor of APMAP gene, carrier, and a cell line stably knocking down the APMAP gene. Background technique [0002] Prostate cancer is the most common malignant tumor of the male urinary system and one of the leading causes of cancer death in men. The incidence of prostate cancer in my country is increasing year by year, and the incidence rate has increased by as high as 13.4% during 1994-2002. In the past ten years, the incidence of prostate cancer has shown a trend of high incidence in China, and effective prevention and control of prostate cancer has become urgent. The clinical stages of prostate cancer are mainly divided into four types. Stage I lesions are completely confined to the prostate and generally have a good prognosis; stage II lesions are confined to the prostate capsule without signs of invasion and metastasis, whic...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574C12N5/10C12N15/867
Inventor 蒋思远高山
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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