Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method of biosynthesis silver nanoparticles

A silver nanoparticle, biosynthesis technology, applied in the field of medicine, can solve the problems of insufficient understanding of the synthesis mechanism, weak targeting, unfavorable application research, etc., to improve synthesis efficiency, high biocompatibility, and overcome drug resistance. Effect

Active Publication Date: 2019-01-29
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, their disadvantages are that such nanocarriers can induce certain immune responses when the blood type does not match, the cell membrane package is everted, or the cell membrane edge is everted; and, for most cell membranes, its own target The tropism is weak, and additional targeting molecules or genetic modification are required to achieve tumor targeting
However, the shortcomings of in vitro biosynthesis of nanomaterials are that the bioreactors used are all cells, and the understanding of the synthesis mechanism is not deep enough, which is not conducive to subsequent application research; The products synthesized by organoids will have immunogenicity and even ethical problems in clinical use, and need to be improved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of biosynthesis silver nanoparticles
  • Preparation method of biosynthesis silver nanoparticles
  • Preparation method of biosynthesis silver nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The invention provides a technical solution: a method for preparing biosynthetic silver nanoparticles, comprising the following steps:

[0029] S1: set 10 7 A HepG2 cell was injected into the nude mouse from the ventral groove of the nude mouse, and a nude mouse model of drug-resistant liver cancer was constructed;

[0030] S2: Isolate T cells from 0.5 mL of blood, and expand culture in 10 mL of culture medium;

[0031] S3: set 10 5 The cells were co-cultured with 0.1 mg silver nitrate in 10 mL medium, and then separated and purified by ultra-high-speed gradient centrifugation (100000 g) and HPLC (flow rate 0.1 mL / min, C18 column) to obtain silver nanoparticles. With near-infrared fluorescence characteristics, serum albumin (Serum albumin, SA) with a molecular mass of 66547 is adsorbed on the surface.

Embodiment 2

[0033] The present invention provides a technical solution: a preparation of biosynthetic silver nanoparticles loaded with microRNA, comprising the following steps:

[0034]S1: Since the surface of biosynthesized silver nanoparticles has been coated with SA, the disulfide bond in SA can be opened by reducing agent, so that it is in an open state;

[0035] S2: Embedding microRNA in it by stirring at a controlled speed;

[0036] S3: After ultra-high-speed separation, the silver nanoparticles loaded with microRNA were precipitated, the supernatant was discarded, and resuspended in water. In water with a low concentration of reducing agent, the disulfide bonds in SA were automatically closed, and the microRNA was encapsulated; When the microRNA silver nanoparticles were in a high-concentration GSH environment, the disulfide bond in SA would automatically open and release the drug.

[0037] Among them, the biosynthetic silver nanoparticles were characterized, and the size of the b...

Embodiment 3

[0039] The present invention provides a technical solution: a preparation of miR-122-loaded biosynthetic silver nanoparticles, comprising the following steps:

[0040] S1: Use the reducing agent glutathione to open the disulfide bond in SA to make it open;

[0041] S2: embedding miR-122 in it by stirring at a controlled speed;

[0042] S3: After ultra-high-speed separation, the silver nanoparticles loaded with miR-122 were precipitated, the supernatant was discarded, and water was added to resuspend. Under the condition of low concentration of GSH, the disulfide bond in SA was automatically closed, and miR-122 was encapsulated ; and when the miR-122-loaded silver nanoparticles were placed in a high-concentration GSH environment in liver cancer cells, the disulfide bond in SA would automatically open and release miR-122.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method of biosynthesis silver nanoparticles, further discloses a preparation method of biosynthesis silver nanoparticles loading microRNA, further discloses application of the biosynthesis silver nanoparticles loading the microRNA to the aspect of preparation of a nano-drug delivery system for targeted induction of drug-resistant hepatocellular carcinoma cellapoptosis, and further discloses the nano-drug delivery system for targeted induction of drug-resistant hepatocellular carcinoma cell apoptosis. According to the drug delivery system, a nanomaterial is synthesized through organism tissue, and thus the biocompatibility of the material is improved; by loading a non-chemotherapy drug, namely the microRNA, the toxic side effects are lowered, multidrugresistance is avoided, and thus the therapeutic effect on drug-resistant hepatocellular carcinoma is improved; and moreover, the drug delivery system also has the advantages of targeting, controlledrelease and visualization, and diagnosis and treatment integration of drug-resistant hepatocellular carcinoma can be achieved.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for preparing biosynthetic silver nanoparticles. Background technique [0002] The application of nano-drug carriers in the field of tumor diagnosis and treatment has attracted increasing attention. However, the unavoidable problem is that in order to integrate various functions into a certain carrier, the nano-drug carrier must go through a complicated synthesis and modification process, which not only greatly increases the technical difficulty, but also has certain functional compatibility. affected. Therefore, it is necessary to construct nano-drug carriers with biocompatibility, controlled drug release, avoidance of drug resistance, tumor targeting and visualization according to the characteristics of tumors, and try to solve the above difficulties while taking into account the simplification of the synthesis steps. [0003] At present, the construction schemes of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K31/7088A61K47/02A61K47/42A61P35/00
CPCA61K9/5169A61K31/7088A61K47/02A61P35/00
Inventor 张根
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL