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Sulfated Polygonum ciliinerve polysaccharide product with effect of preventing piglet diarrhea and application thereof

A technology for piglet diarrhea and sulfation, which is applied to medical preparations containing active ingredients, digestive system, organic active ingredients, etc., can solve problems such as drug residues, intestinal flora imbalance of piglets, and drug resistance of pathogenic microorganisms.

Inactive Publication Date: 2019-01-29
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to control this disease, veterinary clinics have used a large number of antibiotics and chemical drugs for a long time, resulting in the emergence of drug resistance of pathogenic microorganisms, serious drug residues, imbalance of intestinal flora in piglets, and low curative effect. Therefore, it is imminent to develop safe and effective new drugs

Method used

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  • Sulfated Polygonum ciliinerve polysaccharide product with effect of preventing piglet diarrhea and application thereof
  • Sulfated Polygonum ciliinerve polysaccharide product with effect of preventing piglet diarrhea and application thereof
  • Sulfated Polygonum ciliinerve polysaccharide product with effect of preventing piglet diarrhea and application thereof

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Experimental program
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Effect test

Embodiment Construction

[0014] 1. Sulfation modification of cinnabar seven polysaccharides

[0015] (1) Establishment of factor levels

[0016] Combining the literature and preliminary experiments, the reaction temperature, reagent ratio and reaction time have the greatest influence on the modification, so the reaction temperature (A), the ratio of chlorosulfonic acid to pyridine (B) and the reaction time (C) are selected as factors to establish Orthogonal test is carried out at three levels, see Table 1.

[0017] Table 1 Orthogonal test screening conditions for the preparation of sulfated cinnabar seven polysaccharides

[0018]

[0019]

[0020] (2) Preparation of esterification reagent

[0021] Place the three-necked flask with a stirring and condensing device in an ice-salt bath, add 50 mL of pre-cooled anhydrous pyridine, stir to cool, and slowly add chlorosulfonic acid according to the reagent ratio in Table 1, and within 40 min finished adding. After the reaction was finished, 9 kinds...

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Abstract

The invention discloses a preparation method of a sulfated Polygonum ciliinerve polysaccharide product with effect of preventing piglet diarrhea. According to the invention, a chlorosulfonic acid-pyridine method is adopted to conduct sulfating modification on polysaccharide from Polygonum ciliinerve (PCP), an optimal reaction condition is screened out through an orthogonal test, and the optimal reaction condition comprises that the ratio of chlorosulfonic acid to pyridine is 1:8, the reaction temperature is 95 DEG C and the reaction time is 1 h. The obtained product is high in substitution degree and activity. A CCK 8 method is combined with RT-PCR detection to confirm that the sulfated polysaccharide from Polygonum ciliinerve (sPCP) can effectively inhibit replication and proliferation ofporcine transmissible gastroenteritis virus (TGEV), and inhibit Escherichia coli and Shigella, and can improve immune function with an effect significantly better than that of polysaccharide from Polygonum ciliinerve. An optimal drug delivery mode and dosage are screened out. The prepared sulfated Polygonum ciliinerve polysaccharide product can improve the antiviral and immunopotentiating activity of polysaccharide from Polygonum ciliinerve.

Description

technical field [0001] The invention belongs to the technical field of structural transformation of traditional Chinese medicine polysaccharides, and relates to a method for sulfated modification of traditional Chinese medicine polysaccharides, in particular to a sulfated cinnabar heptapolysaccharide product with the effect of preventing and treating diarrhea in piglets and an application thereof. Background technique [0002] Piglet diarrhea is a typical multifactorial infectious disease. The main causes are viral, bacterial and other. It is mainly characterized by diarrhea and high mortality in suckling piglets. It occurs all over the world. To the farmer Cause huge economic losses, seriously threaten the healthy development of pig industry. Porcine transmissible gastroenteritis virus is one of the important pathogens causing diarrhea in piglets. In order to control this disease, veterinary clinics have used a large number of antibiotics and chemical drugs for a long time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/12A61K36/704
CPCA61K31/715A61K36/704A61P1/12C08B37/0003
Inventor 张为民卿素珠鲁孟佳安乐
Owner NORTHWEST A & F UNIV
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