Method for extracting chondroitin sulfate from pig snout cartilage
A technology of chondroitin sulfate and nasal cartilage, which is applied in the field of biological enzymatic hydrolysis technology, can solve the problems of large amount of alkali, difficult purification, and large amount, and achieve the effects of no toxic side effects, high purity, and avoiding residues
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Embodiment 1
[0020] A method utilizing pig nasal cartilage to extract chondroitin sulfate, the steps are as follows:
[0021] 1. Weigh 1 kg of commercially available pig nose cartilage, cut off the connective tissue, wash and boil with water to remove grease, then remove the pig nose cartilage from the water, mince it with a meat grinder, add 3 kg of pure water, and adjust When the pH value reaches 8.5, raise the temperature to 45°C, add 20g of bovine trypsin (enzyme activity is 33,000 U / g), stir and enzymolyze at 45°C for 4 hours, and keep the pH value of the reaction system stable at 8.5 during the enzymolysis process;
[0022] 2. Adjust the pH value of the enzymatic solution to 6.8, add 10g of kaolin and 10g of activated carbon powder in sequence, stir and adsorb at 45°C for 1 hour, then filter to remove the kaolin and activated carbon to obtain the filtrate;
[0023] 3. Adjust the pH value of the filtrate to 6.0, then add three times the volume of 95wt% ethanol, stir well and let it st...
Embodiment 2
[0030] A method utilizing pig nasal cartilage to extract chondroitin sulfate, the steps are as follows:
[0031] 1. Weigh 2kg of commercially available pig nose cartilage, cut off the connective tissue, wash and boil with water to remove grease, then remove the pig nose cartilage from the water, mince it with a meat grinder, add 6kg of pure water, adjust When the pH value reaches 8.6, heat up to 46°C, add 40g of bovine trypsin (enzyme activity is 33,000 U / g), stir and enzymolyze at 46°C for 4.2h, and keep the pH value of the reaction system stable at 8.6 during the enzymolysis process;
[0032] 2. Adjust the pH value of the enzymatic solution to 6.9, add 20g of kaolin and 20g of activated carbon powder in sequence, stir and adsorb at 45°C for 1 hour, then filter to remove the kaolin and activated carbon to obtain the filtrate;
[0033] 3. Adjust the pH value of the filtrate to 6.0, then add three times the volume of 95wt% ethanol, stir thoroughly and let it stand, remove the s...
Embodiment 3
[0040] A method utilizing pig nasal cartilage to extract chondroitin sulfate, the steps are as follows:
[0041] 1. Weigh 10kg of commercially available pig nose cartilage, cut off the connective tissue, wash and boil with water to remove grease, then remove the pig nose cartilage from the water, mince it with a meat grinder, add 30kg of pure water, adjust When the pH value reaches 8.8, raise the temperature to 48°C, add 200g of bovine trypsin (the enzyme activity is 33,000 U / g), stir and enzymolyze at 48°C for 4.5h, and keep the pH value of the reaction system stable at 8.8 during the enzymolysis;
[0042] 2. Adjust the pH value of the enzymatic solution to 6.9, add 100g of kaolin and 100g of activated carbon powder in sequence, stir and adsorb at 45°C for 1 hour, then filter to remove the kaolin and activated carbon to obtain the filtrate;
[0043] 3. Adjust the pH value of the filtrate to 6.0, then add three times the volume of 95wt% ethanol, stir well and let it stand, rem...
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