Tobacco amino acid transporter gene NtTAT and purpose thereof

Abstract

The invention belongs to the technical field of plant gene engineering, and discloses a tobacco amino acid transporter gene NtTAT and a purpose thereof. The contents comprise cloning on the amino acidtransporter gene NtTAT, building on an NtTAT gene interference carrier, expression features after NtTAT gene interference, influence on the tobacco leaf amino acid content, and amino acid content determination on homozygous transgenic later generations. In NtTaT interference homozygous plants, five kinds of amino acids (valine, proline, threonine, phenylalanine and the like) realize the obvious amino acid content increase, wherein the change of the proline is greatest; the increase is nearly 13 times; the contents of valine, threonine and phenylalanine are increased; the NtTAT influences theamino acid metabolism gene through the amino acid transportation capability change; the amino acid content of valine, proline, threonine, phenylalanine and the like in tobacco leaves is improved; andthe quality of the tobacco leaves is further improved.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a tobacco amino acid transporter gene NtTAT and its application. Background technique [0002] At present, the commonly used prior art in the industry is as follows: Nicotiana belongs to the tubular flower family, Solanaceae annual or limited perennial herb, the base is slightly lignified. Inflorescences terminal, paniculate, many-flowered; capsules ovate or oblong, about as long as persistent calyx. It blooms and bears fruit in summer and autumn. Tobacco likes a warm, sunny environment and fertile and loose soil, drought-tolerant, not cold-resistant. Like warm, sunny environment, not cold-resistant, more heat-resistant. Mainly distributed in South America, South Asia and China. Amino acids are a class of important chemical substances in tobacco. During tobacco preparation, alcoholization or fermentation, processing and combustion, enzymatic and ...

AI Technical Summary

Problems solved by technology

Although bioinformatics methods can be used to predict genes with unknown functions, the final function of genes still depends on biological identification.

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