Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for efficient separation of umbilical cord and placental mesenchymal stem cells and application

A technology of placental stem cells and umbilical cords, applied in the field of stem cells, to achieve the effect of improving digestion efficiency

Inactive Publication Date: 2019-02-01
中晶生物技术股份有限公司
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the defects of existing methods for obtaining umbilical cord and placental mesenchymal stem cells, and provide a practical and simple method for isolating a large number of mesenchymal stem cells from umbilical cord and placenta

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for efficient separation of umbilical cord and placental mesenchymal stem cells and application
  • Method for efficient separation of umbilical cord and placental mesenchymal stem cells and application
  • Method for efficient separation of umbilical cord and placental mesenchymal stem cells and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Reagents

[0023] Trypsin, collagenase Ⅱ, Ⅳ, dispase, EDTA, lipase, placental tissue fluid, EGF factor, insulin, hydroxyethyl starch, DMSO were purchased from sigma company. Serum-free MSC medium was made by the company. Protection solution preparation: self-made for the company

[0024] Preparation of digestive juice: trypsin concentration: 0.25%, collagenase II: 0.5mg / ml, collagenase IV: 1mg / ml dispase concentration: 0.1mg / ml.

[0025] 2. Material collection, transportation, separation, amplification and cryopreservation

[0026] The material is taken from the discarded umbilical cord and placenta of healthy pregnant women after childbirth or caesarean section. Pregnant women are required to have a complete health examination report, and they must not have genetic diseases, infectious diseases and carry them. Strictly follow the aseptic operation during the material collection process. The umbilical cord and placenta used must be within 2 hours after delivery or...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Relating to the field of stem cells, the invention preliminarily establishes a system for isolation and culture of umbilical cord and placental stem cells and application. The method includes: (1) treatment and transportation of newborn fetus umbilical cord and placenta; (2) flushing, cutting and digestion treatment of umbilical cord and placenta; (3) rapid and fine treatment of primary cells andtissues; and (4) adding of brand new additive, and passage and liquid change treatment. On the basis of cost saving, the method provided by the invention can achieve more efficient separation and culture of umbilical cord and placental mesenchymal stem cells.

Description

technical field [0001] The present invention relates to the field of stem cells, especially a method for isolating and culturing human umbilical cord and placental mesenchymal stem cells. We add placental tissue fluid to the culture to simulate the original growth environment of the cells to the greatest extent, so that the cells start more stably and proliferate faster. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into two categories according to their developmental stage: embryonic stem cells and adult stem cells. Mesenchymal stem cells (MSCs) are adult stem cells with self-replication ability and multi-lineage differentiation potential, which can develop into bone, cartilage, fat and other types of cells. It can be used as an ideal seed cell for the repair of tissue and organ damage caused by aging and disea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/074C12N5/073
CPCC12N5/0605C12N5/0607C12N2509/00
Inventor 不公告发明人
Owner 中晶生物技术股份有限公司