A microfluidic chip cell culture control device and method

Abstract

The invention discloses a microfluidic chip cell culture control device and method. The cell culture adopts the mode of intermittent perfusion. Firstly, the least squares support vector machine is used to construct the control model of the perfusion interval time, cell density and three-generation time of cell proliferation. The model of cell impedance value resistance and cell growth rate. The microfluidic chip substrate is engraved with an inlet, a sample outlet and a single channel. The inlet and outlet are connected to the syringe pump and the waste liquid pool respectively, controlled by the host computer The syringe pump injects the culture solution into the microfluidic chip, and the initial amount of perfusion is determined through the established control model to prevent the cells from drying out or growing wildly in the initial state. The substrate of the microfluidic chip is provided with interdigitated electrodes, and through the electrode leads The end is connected to the impedance analyzer, the impedance value is measured and the cell growth rate is calculated, the calculated cell growth rate is compared with the growth rate obtained by the model, and the interval time is changed in real time according to the difference, so as to achieve feedback adjustment and monitor the cell state in real time Variety.

Description

technical field [0001] The invention relates to the application field of animal cell culture, in particular to a microfluidic chip cell culture control device and method. Background technique [0002] Cell culture is an essential research process in bioengineering and plays a vital role in various fields such as biology and medicine. Patent application number 201210381418.2 proposes a microfluidic cell culture chip and its real-time observation system, which can update the culture medium in time and monitor the proliferation status of cells in real time. The patent application number 201320697500.6 proposes a cell culture device that can monitor the cell culture liquid environment. The cell culture device can use sensor electrodes to monitor the real-time concentration of a certain substance in the culture liquid in real time, and dynamically study the effect of the culture liquid environment on cells. Although it is all about cell culture, it does not involve the control o...

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Problems solved by technology

The patent application number 201320697500.6 proposes a cell culture device that can monitor the cell culture liquid environment. The cell culture device can use sensor electrodes to monitor the real-time concentration of a certain substance in the culture liquid in real time, and dynamically study the effect of the culture liquid environment on cells. Although they are all about cell culture, they do not involve the control of culture medium parameters. In the process of cell monitoring, it is difficult to cultivate cells regularly, and it is impossible to establish a feedback control link.

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