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A kind of environment-friendly compound microbial liquid bacterial agent and preservation method thereof

A technology of compound microorganisms and liquid bacterial agents, which is applied in the direction of preservation of microorganisms and bacteria, can solve the problems of gas production, reduction of effective microbial mass, rancidity, etc., and achieve the effects of simplified process, sufficient material transfer and comprehensive effects

Active Publication Date: 2020-10-27
GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to a large number of effective microbial bacteria, the fermentation broth also contains protein (peptide) substances secreted by the bacteria, residual carbon sources, nitrogen sources and other nutrients. Therefore, during the storage of liquid microbial agents, there will be Rancidity, gas production, effective microbial biomass will also be greatly reduced

Method used

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  • A kind of environment-friendly compound microbial liquid bacterial agent and preservation method thereof
  • A kind of environment-friendly compound microbial liquid bacterial agent and preservation method thereof
  • A kind of environment-friendly compound microbial liquid bacterial agent and preservation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] In the present embodiment, a kind of preservation method of compound microbial liquid inoculum for environmental protection comprises the following steps:

[0043] (1) Carry out high-density fermentation and culture of compound microorganisms for environmental protection to obtain high-density fermentation broth, centrifuge the high-density fermentation broth at a speed of 4000 rpm, remove the supernatant, and obtain bacterial sludge;

[0044] (2) Dilute the bacterial sludge with 0.2mol / L phosphate buffer solution to obtain the diluted bacterial liquid, adjust the solid content of microorganisms in the diluted bacterial liquid to 10%, and the pH to 5.0;

[0045] (3) After adding a protective agent to the diluted bacterial solution, keep it airtight and store it in a cool and dry place.

[0046] Preferably, the protective agent is composed of p-hydroxybenzoic acid, Tween 20, trehalose and vitamin C, and the amounts of p-hydroxybenzoic acid, Tween 20, trehalose and vitamin ...

Embodiment 2

[0059] In the present embodiment, a kind of preservation method of compound microbial liquid inoculum for environmental protection comprises the following steps:

[0060] (1) Carry out high-density fermentation and culture of compound microorganisms for environmental protection to obtain high-density fermentation liquid, centrifuge the high-density fermentation liquid at a speed of 2500 rpm, remove the supernatant, and obtain bacterial sludge;

[0061] (2) Dilute the bacterial sludge with 0.2mol / L phosphate buffer solution to obtain the diluted bacterial liquid, adjust the solid content of microorganisms in the diluted bacterial liquid to 6%, and the pH to 5.5;

[0062] (3) After adding a protective agent to the diluted bacterial solution, keep it airtight and store it in a cool and dry place.

[0063] Preferably, the protective agent is composed of p-hydroxybenzoic acid, Tween 20, trehalose and vitamin C, and the amounts of p-hydroxybenzoic acid, Tween 20, trehalose and vitam...

Embodiment 3

[0076] In the present embodiment, a kind of preservation method of compound microbial liquid inoculum for environmental protection comprises the following steps:

[0077] (1) Carry out high-density fermentation and culture of compound microorganisms for environmental protection to obtain high-density fermentation liquid, centrifuge the high-density fermentation liquid at a speed of 4500 rpm, remove the supernatant, and obtain bacterial sludge;

[0078] (2) Dilute the bacterial sludge with 0.2mol / L phosphate buffer solution to obtain the diluted bacterial liquid, adjust the solid content of microorganisms in the diluted bacterial liquid to 15%, and the pH to 4.0;

[0079] (3) After adding a protective agent to the diluted bacterial solution, keep it airtight and store it in a cool and dry place.

[0080] Preferably, the protective agent is composed of p-hydroxybenzoic acid, Tween 20, trehalose and vitamin C, and the amounts of p-hydroxybenzoic acid, Tween 20, trehalose and vita...

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Abstract

The invention relates to the technical field of biological fermentation and in particular relates to a compound microorganism liquid bacterium agent for environment protection and a preservation method thereof. The preservation method comprises the following steps: (1) carrying out high-density fermentation culture on a compound microorganism for the environment protection, so as to obtain a high-density fermentation solution; after moderately centrifuging the high-density fermentation solution, removing supernatant to obtain bacterium sludge; (2) diluting the bacterium sludge by utilizing 0.18 to 0.22 mol / L of phosphate buffer solution, so as to obtain a diluted bacterium solution, adjusting the solid content of microorganisms in the diluted bacterium solution to be 6 to 20 percent and the pH (Potential of Hydrogen) to be 4.0 to 6.0; (3) after adding a protection agent into the diluted bacterium solution, sealing and preserving; putting into a shady and dry place. The preservation method provided by the invention is simple to operate and low in cost, and the preservation time of the microorganism liquid bacterium agent is greatly prolonged under the condition that the content of effective microorganisms is ensured.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to an environment-friendly compound microbial liquid bacterial agent and a preservation method thereof. Background technique [0002] At present, most of the microbial inoculum products on the market are in a solid storage state. Although the storage time of solid microbial inoculum products is longer than that of liquid inoculum products, the preparation method has a lot more equipment, processes, and procedures than liquid inoculum products. Labor and cost input. [0003] Most of the general liquid microbial agent products are whole fermentation broth. However, in addition to a large number of effective microbial bacteria, the fermentation broth also contains protein (peptide) substances secreted by the bacteria, residual carbon sources, nitrogen sources and other nutrients. Therefore, during the storage of liquid microbial agents, there will be Rancidity, gas pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12N1/20
CPCC12N1/04C12N1/20
Inventor 张保安王云杨秋婵罗仕宽黄来云
Owner GUANGDONG ZHONGWEI ENVIRONMENTAL PROTECTION BIOTECH CO LTD