New application of Parissaponin VII

A new application technology of papaya saponin, which is applied in the new application field of papaya saponin VII, can solve the problems that papaya papaya saponin VII has not been reported, and achieve the effect of high anti-breast cancer activity, clear effect and good inhibitory effect

Inactive Publication Date: 2019-02-15
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern pharmacological research has found that the steroidal saponins in Chonglou play a major role in medicine, that is, Chonglou saponin, and Chonglou saponin VII is one of them. Anti-tumor and other biological activities, but the application of papaya saponin VII in anti-breast cancer has not been reported yet

Method used

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  • New application of Parissaponin VII
  • New application of Parissaponin VII
  • New application of Parissaponin VII

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Inhibitory effect of saponin VII on breast cancer cells in vitro

[0020] 1. Experimental method

[0021] Human breast cancer cells MCF-7, ZR-75-30, and MDA-MB-231 were grown in MEM medium and RPMI-1640 medium containing 10% fetal bovine serum, 100U / mL penicillin, 100μg / mL streptomycin, respectively , L-15 medium, placed in 5% CO 2 , cultured in a 37°C incubator.

[0022] Take the cells in the logarithmic growth phase and make 5×10 4 cells / ml concentration of cell suspension, 100 μL per well was inoculated in a 96-well plate and cultured for 24 hours. After the cells were fully adhered to the wall, the original medium was discarded, and different concentration gradients of papaya saponin VII prepared from the medium were added. , 100 μL per well. Incubate at 37°C for 24, 48, and 72 hours respectively. After the end of the culture, add 10 μL of 5 mg / mL MTT to each well and continue to culture for 4 hours. Discard the supernatant, add 150 μL of DMSO to each well, and ...

Embodiment 2

[0029] Pauline saponin VII induces apoptosis of breast cancer cells

[0030] 1. Experimental method

[0031] MCF-7, ZR-75-30, MDA-MB-231 cells in the logarithmic growth phase were collected, digested with 0.25% trypsin without EDTA, seeded in 6-well plates, and adjusted the number of cells to 1.0×10 per well 5 After culturing for 24 hours, the culture medium was discarded, and the cells were treated with saponin VII (concentrations of 12, 10, and 36 μmol / L, respectively) for 24 hours. At the same time, the Annexin V-FITC and PI single-stained wells of 0h were set as controls. After 24 hours, transfer the cell supernatant to a 15ml centrifuge tube, wash twice with PBS, add EDTA-free trypsin to digest the cells, then transfer to a centrifuge tube, add medium to stop digestion, centrifuge at 500×g for 5 minutes, remove The supernatant was drained upside down on filter paper. The cells were resuspended in 1 mL of PBS and transferred to a 1.5 mL EP tube, and then centrifuged at ...

Embodiment 3

[0035] In vivo anti-breast cancer xenograft tumor experiment of Patricia saponin VII

[0036] 1. Experimental method

[0037] Take BALB / c mice, raise the animals in separate cages, and start the experiment after 3 days of adaptation to the environment. Take the mouse 4T1 breast cancer cells in the logarithmic growth phase, digest with trypsin, centrifuge, remove the supernatant, count, and adjust the cell concentration to 2×10 with normal saline 7 Cells / ml, the cells were inoculated subcutaneously in the axilla of the right forelimb of the mouse, 0.1ml / mouse, and recorded as the first day of inoculation. Record the long diameter (a), short diameter (b) and body weight of the transplanted tumor every 2 days, and calculate the tumor volume (V) according to the following formula, V=a×b 2 / 2. When the tumor grows to 100mm 3 At the same time, the animals were randomly divided into 4 groups, 6 in each group: (1) blank control group: intraperitoneal injection of normal saline, 0....

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Abstract

The invention discloses new application of Parissaponin VII. The new application has a preferable inhibiting effect on the growth of breast cancer cell strains and mouse transplantation tumor, and theapoptosis of breast cancer cells can be induced. The Parissaponin VII is high in breast caner activity resistance and obvious in effect, and simultaneously can be matched with a clinically accepted excipient, so that oral preparations (tablets, tinctures, dispersible powder, granules, emulsions, syrups, hard capsules, soft capsules and the like) or non-oral preparations (injections, sprays, pastes and the like) are prepared for the treatment of breast cancer.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and relates to a new application of papaya saponin VII. Background technique [0002] Breast cancer is one of the common malignant tumors that threaten women's health. Its incidence rate ranks first in female malignant tumors, and it is increasing year by year. In my country, the growth rate of the incidence of breast cancer is 1 to 2 percentage points higher than that of Western countries. Only from 2003 to 2009, the mortality rate of breast cancer in Chinese cities increased by 38.91%. According to the results of "2015 China Cancer Statistics", the incidence rate (272.4 per 100,000) and mortality rate (70.7 per 100,000) of breast cancer are both at a relatively high level, becoming the leading cause of cancer death in women under the age of 45. For breast cancer patients, when the lesion develops to an advanced stage, about 50% of breast cancer will metastasize and spread, resulting in multip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00
CPCA61K31/7048A61P35/00
Inventor 何昊许畅徐玥成昭郑蕾
Owner XIAN MEDICAL UNIV
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