Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Microfluidic free flow paper chromatographic array spray mass spectrometry device

A technology of microfluidics and paper chromatography, which is applied in the field of chromatography-mass spectrometry, can solve the problems of low separation efficiency and time-consuming, and achieve the effects of improving separation efficiency, saving space, and simple design

Active Publication Date: 2019-02-15
FUDAN UNIV
View PDF8 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current glycoprotein separation methods are mainly liquid chromatography, gel chromatography, solid phase extraction and other methods. Among these methods, some have problems such as low separation efficiency and some have time-consuming problems.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microfluidic free flow paper chromatographic array spray mass spectrometry device
  • Microfluidic free flow paper chromatographic array spray mass spectrometry device
  • Microfluidic free flow paper chromatographic array spray mass spectrometry device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 , As shown in 2, 3, the microfluidic free paper chromatography array spray mass spectrometry device includes a microfluidic control device 14, a microfluidic free flow paper chromatographic separation device that is a microfluidic carrier plate 4, a microfluidic cover plate 6, Paper stationary phase 10, and array spray component 11, mass spectrometry component. In this embodiment, the paper stationary phase 10 is placed in the separation cavity 2 formed by the microfluidic carrier plate 4 and the microfluidic cover plate 6 to ensure that the device is sealed. The buffer or mobile phase enters the separation chamber 2 through the first pipe 1 through the microfluidic channel 7, and the sample enters the separation cavity 2 through the second pipe 3 through the sample channel 8; in this embodiment, microfluidic control is used The device 14 controls the sample loading and the flow control of the buffer or mobile phase. The microfluidic control device 14 c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
widthaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention relates to a microfluidic free flow paper chromatographic array spray mass spectrometry device. The device comprises a microfluidic control device, a microfluidic separation device, an array spray component and a mass spectrum component, wherein the microfluidic control device is used for controlling sample loading and flow of a mobile phase or a buffer solution. The microfluidic separation device is composed of a microfluidic support plate, a microfluidic cover plate and a paper stationary phase, wherein a metal electrode used for providing a separated electric field is plated on the support plate; a channel used for sample loading and mobile phase transport is reserved in the front end of the cover plate; a separating cavity for separating and filling the paper stationary phase is reserved in the middle of the cover plate; a fraction outlet is formed in the tail end of the separating cavity; the separating cavity is filled with the paper stationary phase and the paper stationary phase is used for separating the target object; the fraction outlet is connected with an array spray device; and mass spectrometry is performed after spray of the array spray device. According to the device disclosed by the invention, the advantages of free flow electrophoresis, chromatography and mass spectrometry are combined, the separation detection efficiency of the traditional freeflow electrophoresis can be very effectively improved, separation is performed in a transverse direction by utilizing the electric field effect, and the separating effect is further achieved in the longitudinal direction according to a chromatographic mechanism.

Description

Technical field [0001] The invention relates to the field of chromatography and mass spectrometry, in particular to a microfluidic free-flow paper chromatography array spray mass spectrometry device. Background technique [0002] In the past 30 years, two-dimensional separation and analysis technology has developed rapidly and has been widely used in the separation and analysis of complex biological, chemical, medical, and environmental samples. The biggest advantage of this separation analysis method is that it has a very high peak capacity. So far, there have been many two-dimensional separation methods, including capillary electrophoresis-high performance liquid chromatography, capillary electrophoresis-capillary electrophoresis, etc. Good results. Based on the advantages of high integration, high throughput, and low consumption, microfluidic chip technology has become one of the important means of life science research. It uses the chip as the basic platform to establish a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N30/02G01N30/72
CPCB01L3/5023B01L3/502753G01N30/02G01N30/72
Inventor 刘颖超张权青李顺祥杨芃原贾滨
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products