Preparation method of selenium-rich germanium-rich bio-additive for beef cattle
A technology of biological additives, selenium and germanium, which is applied in the direction of preservation of microorganisms, animal feed, animal feed, etc., can solve problems such as the difficulty of separating organic selenium and germanium, and achieve the effects of ensuring biological food safety, improving immunity, and increasing content
Inactive Publication Date: 2019-02-19
珲春市龙裕农业发展集团有限公司
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Problems solved by technology
[0003] The purpose of the present invention is to solve the shortcoming that there is difficulty in separating organic selenium and germanium in the prior art, and the preparation method of the selenium and germanium double-enriched beef cattle biological additive proposed
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Embodiment
[0019] Embodiment: The preparation method of selenium-germanium double-enriched beef cattle biological additive includes the following steps:
[0020] S1. Strain activation: from the highly tolerant yeast strains stored in the laboratory, use a loop to inoculate the highly tolerant metal strain in a test tube containing 5ml of liquid yeast seed synthetic medium (liquid yeast The composition of the seed synthesis medium is: glucose 100g / L, tryptone 10g / L, sodium chloride 10g / L, yeast extract 2g / L, urea 2g / L, magnesium sulfate 0.5g / L, and adjust liquid yeast seeds The pH value of the synthetic medium is 7.2), shake culture in a shaker at 30°C and 200r / min for 18h;
[0021] S2. Strain expansion culture: Inoculate the highly tolerant yeast strain activated in 1ml S1 in a 100ml conical flask of liquid yeast seed synthetic medium, and cover the mouth of the conical flask with good air permeability The cotton plug is cultured in a shaker at 30°C and 200r / min for 24h;
[0022] S3. Inoculat...
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The invention discloses a preparation method of a selenium-rich germanium-rich bio-additive for beef cattle. The preparation method includes the steps of S1, activating via a bacterial strain; S2, performing enlargement culture of the bacterial strain; S3, inoculating 10 ml of high-tolerant yeast strain subjected to enlargement culture in S2 to a 1000 ml conical flask with rice bran fermentation medium containing selenium and germanium, performing shake culture for 2 days at 30 DEG C in a shaking bed at the speed of 200 r / m so as to obtain a yeast culture with enriched selenium and germanium;S4, centrifugally treating the yeast culture with enriched selenium and germanium in a centrifuge at the speed of 3000 rpm / min, removing supernate, collecting precipitating strain, adding a compositeprotectant to the precipitating strain, and making the precipitating strain into lyophilized powder that is the selenium-rich germanium-rich bio-additive for beef cattle. A yeast is used to enrich selenium and germanium; inorganic selenium and germanium are converted into organic selenium and germanium via the yeast; after being taken by beef cattle, the organic selenium and germanium never harm the beef cattle; therefore, bio-food safety is substantially guaranteed.
Description
Technical field [0001] The invention relates to the technical field of biological additives, in particular to a preparation method of a biological additive for selenium-germanium double-enriched beef cattle. Background technique [0002] The preparation method of selenium-germanium double-enriched beef cattle biological additives can improve the quality of beef and the content of antioxidants in beef cattle, prevent diseases, and also increase the deposition of selenium and germanium in beef myoglobin, so that the beef has health care effects. About selenium and germanium There are many separate reports on the effects of elements, especially the research on selenium-enriched yeast, but there are few reports on the combined effects of the two in animal production, and they are limited to basic research in the laboratory of compound trace elements. The existing separation of organic selenium and germanium is difficult, but the biotransformation amount of organic selenium and german...
Claims
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IPC IPC(8): C12N1/16C12N1/04A23K50/10A23K10/18A23K20/20
CPCA23K10/18A23K20/30A23K50/10C12N1/04C12N1/16
Inventor 陈石婷张敏耿春银韩东魁国传福丁洋陈新刘茜妍王青海柳丛车俊东马国民陈丽萍
Owner 珲春市龙裕农业发展集团有限公司