A kind of preparation method and device of autologous platelet-rich plasma for the treatment of pressure injury

Abstract

The invention discloses a method and device for preparing autologous platelet-rich plasma for treating pressure injuries. The preparation method includes: ① whole blood collection; ② adding anticoagulant; ③ one-time centrifugation; ④ two-time centrifugation; ⑤ three-time centrifugation; ⑥ Obtain platelet-rich plasma; the preparation device includes a centrifuge tube, the centrifuge tube includes an outer tube, an inner tube and a sealing cover, the inner wall of the outer tube and the outer wall of the inner tube are closely attached, and the sealing cover is sealed and connected with the upper ends of the outer tube and the inner tube, The bottom of the inner tube is provided with a drain tube, and a simple clip is set on the drain tube. There are multiple suction holes on the side wall of the inner tube, and a side wall seal that can be penetrated by a needle is provided in the suction hole. , the preparation device is simple in structure and easy to operate. During the preparation process of the product, the probability of blood contamination can be greatly reduced. The concentration of platelets in the prepared product is high, the extraction efficiency is high, the preparation cycle is short, and the feasibility Strong, and has clinical application value.

Description

technical field [0001] The invention belongs to the technical field of platelet-rich plasma extraction, and in particular relates to a preparation method and device for autologous platelet-rich plasma for treating pressure injuries. Background technique [0002] Common pressure injuries include pressure sores and decubitus ulcers, which are caused by long-term pressure on local tissues, malnutrition, and blood circulation disorders, resulting in continuous ischemia and hypoxia of local tissues, which eventually lead to local tissue necrosis. Nursing of pressure injuries is difficult , the treatment is more difficult. Autologous platelet-rich plasma, referred to as PRP, is the plasma containing high concentration of platelets obtained by centrifugation of autologous whole blood, and platelets contain a large number of growth factors, such as platelet-derived growth factor, transforming growth factor, insulin-like growth factor, epidermal growth factor These growth factors ca...

AI Technical Summary

Problems solved by technology

[0003] The concentration of platelets in human whole blood is generally 1-3×10 5 / ml, studies have shown that the increase in the concentration of concentrated PRP platelets can effectively improve the proliferation and differentiation capabilities of stem cells, and can also significantly increase the proliferation of fibroblasts and the expression of type I collagen. At present, there are manual methods and equipment preparation methods for the extraction of PRP , the existing problems are as follows: one is that the extraction method is more cumbersome, the equipment is expensive and the operation is complicated; the other is that the PRP concentration after extraction is not high, the purity is not good, and it is easily polluted by the outside during the extraction process; the third is that the extraction efficiency is low , these problems make PRP limited in the clinical treatment of pressure injury

Images