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A kind of method maintaining the vigor of Flammulina velutipes strain

A technology of Flammulina velutipes and vitality, which is applied in the field of Flammulina velutipes strain viability maintenance, can solve problems such as consistency and inability to guarantee the strain vitality, and achieve the effects of maintaining vitality, high vitality, and simple and easy operation.

Active Publication Date: 2022-02-01
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can improve the viability of the strains to a certain extent, it is often impossible to ensure that the purified and rejuvenated strains are consistent with the original strains in practical applications.

Method used

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  • A kind of method maintaining the vigor of Flammulina velutipes strain
  • A kind of method maintaining the vigor of Flammulina velutipes strain
  • A kind of method maintaining the vigor of Flammulina velutipes strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: Flammulina velutipes 'Nongjin No. 7' monokaryon acquisition

[0045] Flammulina velutipes 'Nongjin No. 7' was selected by the team of the Fungal Research Center of Fujian Agriculture and Forestry University, and was certified by the Fujian Provincial Crop Variety Approval Committee in 2016.

[0046] Flammulina velutipes ‘Nongjin No. 7’ was activated twice on PDA medium and cultured in a constant temperature biochemical incubator at 25°C for 5 days;

[0047] The activated 'Nongjin No. 7' was inoculated in potato glucose liquid medium, kept at a constant temperature of 25°C, and cultured statically for 4 days;

[0048] In the ultra-clean workbench, pick fresh mycelium into a sterile 1.5mL centrifuge tube, add 1 mL of sterilized 0.5 mol / L KCl, centrifuge at 8000 rpm / min for 10 min, discard the supernatant, and repeat once;

[0049] Add 1 mL of 0.5 mol / L KCl containing 2 wt.% lysozyme, and enzymatically hydrolyze in a water bath at 30°C for 1.5 h;

[0050] F...

Embodiment 2

[0057] Embodiment 2: Flammulina velutipes 'Nongjin No. 7' regenerated bacterial strain obtains, comprises the following steps:

[0058] The Flammulina velutipes 'Nongjin No. 7' strain was taken out from the storage library, transferred to PDA medium, and cultured at 25°C for 6 days.

[0059] The monokaryotic strain 'FNJ7D-1' of Flammulina velutipes 'Nongjin 7' was taken out from the storage library, transferred to PDA medium, and cultured at 25°C for 6 days.

[0060] Take a piece of fresh 'Nongjin No. 7' bacterial block on a plate with a diameter of 9 cm containing PDA medium, and place it in the center, and take a piece of fresh 'Nongjin No. 7' monokaryotic strain 'FNJ7D-1' On the other side of the center of the plate, about 1.5 cm away from the 'Nongjin No. 7' bacterial block, place it in a 25°C constant temperature biochemical incubator on the front.

[0061] After the two hyphae were in contact, continue to cultivate for 4 days. Under aseptic operation, take the bacterial...

Embodiment 3

[0063] Embodiment 3: Flammulina velutipes 'Nongjin No. 7' regenerated strain fruiting test comprises the following steps:

[0064] The regenerated strain 'NJ7-1' of 'Nongjin No. 7' was expanded and cultivated on PDA medium, and the original seed was made. After the original seed grew well, it was transferred to the conventional medium for cultivation test. The cultivation was carried out in industrial bacteria bags. When fruiting, the regeneration method is used for fruiting. See the 45th chapter of Flammulina velutipes in "Chinese Edible and Medicinal Mycology" edited by Huang Nian et al. . (Huang Nian, Lin Zhibin, Chen Guoliang, 2010. Flammulina velutipes. Chinese edible and medicinal mycology. Shanghai: Shanghai Science and Technology Press.)

[0065] The regenerated 'Nongjin No. 7' mycelium has high vigor, and on the cultivation material, the mycelium is white, strong, and fungus grows quickly ( figure 2 ), the full bag time is 29-31 days; primordia are easy to form af...

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Abstract

The invention provides a method for maintaining the vitality of Flammulina velutipes strains. The technical scheme adopted includes protoplast monokaryonization of the heterokaryotic strains of Flammulina velutipes, obtaining the monokaryotic strains, and carrying out conventional preservation respectively. When using the heterokaryotic strains of Flammulina velutipes Pair it with its monokaryotic strain to obtain its regenerated heterokaryon strain, so that the vitality of the Flammulina velutipes strain can be effectively maintained. On the basis of obtaining the monokaryonized bacterial strain, the method only needs to re-match the original variety with its monokaryotic bacterial strain to obtain a new-born variety. The new variety obtained in this way has no change in the genetic material, maintains the characteristics of the original variety, and has a high strain vitality. The operation is simple and easy, and the vitality of the bacteria can be effectively maintained.

Description

technical field [0001] The invention belongs to the field of Flammulina velutipes, and more relates to a method for maintaining the vitality of Flammulina velutipes strains. Background technique [0002] Flammulina velutipes belongs to the fungus of quadrupolar heterothalism, the original Latin name is Flammulina velutipes In 2018, Yang Zhuliang's research team, in cooperation with domestic and foreign counterparts, discovered that the "Enoki mushroom" domesticated from "Winter mushroom" in East Asia is two different species from the "Enoki mushroom" originally described in Europe. For this reason, the team gave "Winter mushroom" or "Enoki mushroom" has a scientific name, that is, "flammulina velutipes" F. filiformis ". [0003] Flammulina velutipes is a kind of edible fungus that we are familiar with. It is not only delicious, but also has various functions such as anti-oxidation and improving immunity. It is the first fungus to realize industrial cultivation in my coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/06C12R1/645
CPCC12N1/06C12N1/14
Inventor 刘新锐江玉姬吴小平谢宝贵
Owner FUJIAN AGRI & FORESTRY UNIV
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