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Xupu goose fatty liver fat deposition a-fabp gene and method of genetic marker

A genetic marker, Xupu goose technology, used in genetic engineering, plant genetic improvement, botanical equipment and methods, etc.

Active Publication Date: 2021-10-22
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on DNA genetic markers, genetic diversity and related functional genes of Xupu goose is still in its infancy, and the genetic markers related to fatty liver fat deposition in Xupu goose have not been reported yet.

Method used

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  • Xupu goose fatty liver fat deposition a-fabp gene and method of genetic marker
  • Xupu goose fatty liver fat deposition a-fabp gene and method of genetic marker
  • Xupu goose fatty liver fat deposition a-fabp gene and method of genetic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 SNP screening of genes related to fat deposition in fat liver of Xupu goose

[0055] Sixty healthy Xupu geese of the same age were selected for the filling-feeding experiment. After 28 days of slaughter, the livers of Xupu geese were weighed, and the genomic DNA was extracted from extreme samples (large and small livers).

[0056] According to the goose A-FABP gene sequence in GenBank, Primer5.0 was used to design primers covering the entire sequence of exon 2 to exon 3 of the gene. The primer sequence is: 5'-CCCAAATAAGATAAGAACAC-3' (forward), 5' -ACATCCACCAGACAAGAA-3' (reverse), synthesized by Hunan Qingke Biotechnology Co., Ltd. The genomic DNA of Xupu goose was amplified with the above primers respectively.

[0057] PCR: 20 μl reaction system: 12 μl of 2×Master mix, 0.8 μl each of upstream and downstream primers (10 μmol / L), 1 μl of DNA template, and make up to the required volume with ultrapure water.

[0058] PCR amplification program: pre-denaturation...

Embodiment 2

[0062] Example 2 Verification of SNPs related to fat deposition in Xupu goose fat liver in production practice

[0063] Twenty-one healthy Xupu geese aged 80 days and under the same feeding and management conditions were selected for the experiment. After 28 days of feeding, all the tested Xupu geese were slaughtered, and the weight of the liver was weighed.

[0064] Genomic DNA extracted from 21 Xupu geese pectoralis tissue samples was amplified using the primers in Example 1, and the SNPs were detected according to the method in Example 1.

[0065] 1. PCR

[0066] The PCR reaction system is shown in the table below:

[0067] Table 1 PCR reaction system

[0068]

[0069] The PCR cycling parameters are as follows:

[0070] Pre-denaturation at 95°C for 5min; denaturation at 95°C for 30s, annealing at 50.3°C for 30s, extension at 72°C for 1min, a total of 35 cycles; extension at 72°C for 10min, and finally storage at 4°C.

[0071] 2. Analysis of genotype frequency of A-F...

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Abstract

The invention discloses a fat-type fatty acid binding protein gene A-FABP (intron 2) and a genetic marker used as a trait of fat liver of Xupu goose. The Xupu goose A-FABP is screened by measuring the size of fat liver of Xupu goose FABP gene polymorphism, there is a C→T mutation at the 186bp of the sequence of SEQ ID NO: 1 of the A-FABP gene, resulting in the existence of a polymorphism. Then, the association analysis between the polymorphism of the A-FABP gene and the traits of fatty liver revealed that the A-FABP gene of Xupu goose, as shown in the sequence SEQ ID NO: 1, has a C / T base mutation at 186bp, and the CC genotype The liver size of Xupu goose was significantly higher than that of other genotypes. The invention provides molecular markers for marker-assisted selection of Xupu goose fat liver traits.

Description

technical field [0001] The invention belongs to the field of poultry molecular biotechnology and breeding, and mainly detects genetic markers related to the traits of fat liver of Xupu goose, specifically detects polymorphisms in the A-FABP gene, that is, the traits related to the traits of fat liver of Xupu goose The presence of a single nucleotide polymorphism (Singlenucleotide polymorphism, abbreviated as SNP) in the fatty acid binding protein gene. [0002] technical background [0003] Xupu goose is a local breed in my country. It has the best fat liver performance, large size, fast growth, and strong disease resistance. It is particularly obvious that it belongs to the national genetic resources of local breeds. In 2014, it entered the national genetic resources of livestock and poultry. protected list. Goose fatty liver is one of the three treasures in the world-class high-end delicious health food. It is rich in unsaturated fatty acids, linoleic acid, lecithin, etc., ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/12C12Q1/6888
CPCC07K14/465C12Q1/6888C12Q2600/124C12Q2600/156
Inventor 曲湘勇郭松长贺长青柳序
Owner HUNAN AGRICULTURAL UNIV
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