Preparation method for metal organic framework material-heme-based electrochemical sensor and application thereof

A metal-organic framework and heme technology, applied in the direction of material electrochemical variables, chemical instruments and methods, organic compounds/hydrides/coordination complex catalysts, etc., can solve the problem of poor catalytic activity and stability, and poor catalytic activity of heme , poor stability of heme, etc., to achieve the effects of fast sensitivity, high electrochemical catalytic activity, enhanced stability and catalytic activity

Inactive Publication Date: 2019-02-22
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, heme is insoluble in water, and the heme immobilized on the electrode is easily detached from the electrode surface by simple physical adsorption.
Secondly, it is easy to polymerize in aqueous solution and destroy the dimer of the oxidizing medium, resulting in poor catalytic activity and stability. In the prior art, there is a lack of suitable materials to fix heme to form a stable electrochemically active interface to prevent Dimerization of heme, thus, poor stability of heme and poor catalytic activity of heme

Method used

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  • Preparation method for metal organic framework material-heme-based electrochemical sensor and application thereof

Examples

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Effect test

Embodiment 1

[0025] A preparation method based on a metal organic framework material-heme electrochemical sensor is carried out as follows:

[0026] 1) Synthesis of MOFs: Weigh 0.166 g of terephthalic acid and 0.096 g of nickel nitrate hexahydrate, mix them in 20 mlml N,N-dimethylformamide (DMF), and add 2 ml of 0.4 M NaOH solution, closed the reaction vessel, and reacted at 100°C for 8 h; after the reaction, cooled to room temperature, washed the obtained precipitate with DMF and absolute ethanol three times, and centrifuged and dried to obtain nickel-MOFs nano Material.

[0027] 2) Preparation of MOFs-heme nanocomposites: Weigh 0.1 g hemin and dissolve it in 30 ml DMF, then add 4 ml freshly prepared 0.1 M NaOH solution, mix well, sonicate for 30 min, and then add 0.1 g MOFs Sonicate for 3 min, then stir for 15 min, close the reactor, and place it in an oven at 100°C for 24 h. The obtained precipitates were washed three times with DMF and absolute ethanol, and dried to obtain pure MOFs-...

Embodiment 2

[0036] A preparation method based on a metal organic framework material-heme electrochemical sensor, comprising the steps of:

[0037] 1) Synthesis of MOFs: Mix terephthalic acid and nickel nitrate hexahydrate in DMF, add NaOH solution drop by drop under stirring conditions, close the reaction vessel, and react at 100±5°C for 7-9 h; After finishing, cool down to room temperature, wash the obtained precipitate with DMF and absolute ethanol for more than 3 times, and obtain nickel-MOFs nanomaterials after drying.

[0038] In this step, the mol ratio of terephthalic acid, nickel nitrate hexahydrate and NaOH is 3: 0.95: 2.3; the consumption of DMF is 70 times of the quality sum of terephthalic acid and nickel nitrate; The molarity is 0.35 mol / l.

[0039] 2) Preparation of MOFs-heme nanocomposites: Mix hemin and MOFs at a mass ratio of 1:0.95, dissolve in DMF, add NaOH solution to it, react at 100±5°C for 18 hours, and cool to room temperature; The obtained precipitates were wash...

Embodiment 3

[0049] A preparation method based on a metal organic framework material-heme electrochemical sensor, comprising the steps of:

[0050] 1) Synthesis of MOFs: Mix terephthalic acid and nickel nitrate hexahydrate in DMF, add NaOH solution drop by drop under stirring conditions, close the reaction vessel, and react at 105°C for 9 h; after the reaction, cool After reaching room temperature, the obtained precipitates were washed three times with DMF and absolute ethanol, and dried to obtain nickel-MOFs nanomaterials.

[0051] In this step, the mol ratio of terephthalic acid, nickel nitrate hexahydrate and NaOH is 3: 0.95: 2.5; the consumption of DMF is 74 times of the quality sum of terephthalic acid and nickel nitrate; The molarity is 0.35 mol / l.

[0052] 2) Preparation of MOFs-heme nanocomposites: Mix hemin and MOFs at a mass ratio of 1:0.95, dissolve in DMF, add NaOH solution to it, react at 105°C for 30 h, and cool to room temperature; The obtained precipitates were washed thr...

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Abstract

The invention relates to a preparation method for a metal organic framework material-heme-based electrochemical sensor and an application thereof in the field of electrochemical sensors. The preparation method comprises the following steps: synthesizing MOFs first and then preparing an MOFs-heme nano compound; and then preparing a solution, dropwise coating the solution to a glass-carbon electrodeafter surface treatment, and airing the electrode to obtain the MOFs-heme modified electrode sensor. The sensor can be used for detecting content of hydrogen peroxide in water quantitively. The detection comprises the following steps: first, detecting and drawing a standard curve; and determining a current response value of a to-be-detected sample solution, and converting the content of hydrogenperoxide in the to-be-detected sample solution according to the standard curve. The sensor solves the problem that an independent heme modified electrode is easy to fall and polymerize, improves the catalytic activity and stability of the electrode and is low in cost and convenient to use. The sensor has a wide application prospect in the fields of environment protection, food safety, clinical medicine and the like.

Description

technical field [0001] The invention relates to the technical field of preparation of electrochemical sensors, in particular to a preparation method and application of a hydrogen peroxide electrochemical sensor based on a metal organic framework material-heme nanocomposite. Background technique [0002] hydrogen peroxide (H 2 o 2 ), commonly known as hydrogen peroxide, has strong oxidizing, bleaching, disinfection and sterilization properties, so it is widely used in industrial production, biopharmaceuticals, clinical applications, environmental engineering and beauty industries. However, the abuse of hydrogen peroxide will have great side effects on the human body, directly stimulate the mucosal tissue, damage human cells, accelerate the aging of human cells, cause various diseases, and even release active oxygen free radicals through chemical reactions to cause cell canceration. Therefore, the development of simple, rapid and highly sensitive hydrogen peroxide detection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/42B01J31/22
CPCB01J31/2239B01J2531/847G01N27/327G01N27/423
Inventor 舒韵陈敬媛徐泽胡效亚
Owner YANGZHOU UNIV
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