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Application of composite fluorescent probe in imaging detection of live tumor cells

A composite fluorescence and cell imaging technology, applied in the direction of fluorescence/phosphorescence, luminescent materials, measuring devices, etc., can solve the problems of application limitations, high biological toxicity of quantum dots, etc., and achieve low biological toxicity, good cell membrane permeability, and good water solubility sexual effect

Active Publication Date: 2019-02-26
重庆问创基因科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, in the application of biological imaging, due to the high biological toxicity of conventional quantum dots, its application in in vivo imaging is limited.

Method used

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  • Application of composite fluorescent probe in imaging detection of live tumor cells
  • Application of composite fluorescent probe in imaging detection of live tumor cells
  • Application of composite fluorescent probe in imaging detection of live tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0010] The synthetic route of 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile:

[0011]

[0012] Synthetic steps:

[0013] 1. Add 200mg of 5-bromoacenaphthenequinone and 125mg of diaminomaleonitrile into a 50mL two-necked flask, then add 6mL of glacial acetic acid, stir and heat under reflux at 120°C for 2h, cool in an ice-water bath, filter, dry, and column layer Analysis and separation gave bright yellow 3-bromo-acenaphthopyrazine-8,9-dicarbonitrile;

[0014] 2. Take 100mg of 3-bromo-acenaphthopyrazine-8,9-dinitrile and 40mg of 4-aminobutyric acid in a 50mL round bottom flask, add 8mL of dimethylsulfoxide, heat and reflux at 110°C for 1h, the reaction forms The red mixture can be distilled under reduced pressure to obtain 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile;

[0015] 3. Add 6mg of 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile into a 50mL two-necked flask, then add 25mL of dimethylsulfoxide and stir to dissolve, ...

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Abstract

The invention discloses an application of a composite fluorescent probe in imaging detection of live tumor cells. When the composite fluorescent probe is used in imaging detection of live tumor cells,in a waveband of 580 to 620 nm, the composite fluorescent probe generates prominent fluorescent signals in live tumor cells, and the composite fluorescent probe is a 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dinitrile compounded titanium dioxide quantum dot. Titanium dioxide has a strong light absorbing performance, energy absorbed by titanium dioxide is transmitted to 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dinitrile, the emitting strength of the quantum dot is enhanced, strong fluorescent signals are obtained, organic substances are gathered on titanium dioxide nano particles,the water solubility of the quantum dots is improved, and moreover, the fluorescent probe has a good performance on penetrating the cell membranes.

Description

technical field [0001] The invention belongs to the application technical field of fluorescent probes, and in particular relates to the application of a composite fluorescent probe in the imaging detection of tumor live cells. Background technique [0002] Compared with traditional dye molecules in the field of biological imaging, quantum dots do have several advantages. Inorganic microcrystals can withstand multiple excitations and light emissions, while organic molecules will decompose. The long-lasting stability allows researchers to observe cells and tissues for a longer period of time and perform interface modification and connection without difficulty. The biggest advantage of quantum dots is that they have rich colors. The complexity of biological systems often requires simultaneous observation of several components. If dyed with dye molecules, different wavelengths of light are required for excitation, but quantum dots do not have this problem. Nanocrystals of diffe...

Claims

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Application Information

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IPC IPC(8): C07D241/38C09K11/06C09K11/67G01N21/64
CPCG01N21/6428C09K11/06C09K11/671C07D241/38C09K2211/1044
Inventor 李一达
Owner 重庆问创基因科技有限公司
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