Application of composite fluorescent probe in imaging detection of live tumor cells

A composite fluorescence and cell imaging technology, applied in the direction of fluorescence/phosphorescence, luminescent materials, measuring devices, etc., can solve the problems of application limitations, high biological toxicity of quantum dots, etc., and achieve low biological toxicity, good cell membrane permeability, and good water solubility sexual effect

Active Publication Date: 2019-02-26
重庆问创基因科技有限公司
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AI-Extracted Technical Summary

Problems solved by technology

However, in the application of biological imaging, due to the high biological tox...
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Abstract

The invention discloses an application of a composite fluorescent probe in imaging detection of live tumor cells. When the composite fluorescent probe is used in imaging detection of live tumor cells,in a waveband of 580 to 620 nm, the composite fluorescent probe generates prominent fluorescent signals in live tumor cells, and the composite fluorescent probe is a 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dinitrile compounded titanium dioxide quantum dot. Titanium dioxide has a strong light absorbing performance, energy absorbed by titanium dioxide is transmitted to 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dinitrile, the emitting strength of the quantum dot is enhanced, strong fluorescent signals are obtained, organic substances are gathered on titanium dioxide nano particles,the water solubility of the quantum dots is improved, and moreover, the fluorescent probe has a good performance on penetrating the cell membranes.

Application Domain

Organic chemistryFluorescence/phosphorescence +1

Technology Topic

PhysicsWater soluble +11

Image

  • Application of composite fluorescent probe in imaging detection of live tumor cells
  • Application of composite fluorescent probe in imaging detection of live tumor cells
  • Application of composite fluorescent probe in imaging detection of live tumor cells

Examples

  • Experimental program(1)

Example Embodiment

[0009] Example
[0010] The synthetic route of 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile:
[0011]
[0012] Synthesis steps:
[0013] 1. Add 200 mg of 5-bromoacenaphthoquinone and 125 mg of diaminomaleonitrile to a 50 mL two-necked flask, then add 6 mL of glacial acetic acid, stir and heat under reflux at 120 °C for 2 h, cool in an ice-water bath, filter, dry, and then the column layer The bright yellow 3-bromo-acenaphthopyrazine-8,9-dicarbonitrile was obtained by analysis and separation;
[0014] 2. Take 100 mg of 3-bromo-acenaphthopyrazine-8,9-dicarbonitrile and 40 mg of 4-aminobutyric acid into a 50 mL round-bottomed flask, add 8 mL of dimethyl sulfoxide, heat under reflux at 110 °C for 1 h, the reaction generates The red mixture can be distilled under reduced pressure to obtain 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile;
[0015] 3. Add 6 mg of 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile to a 50 mL two-necked flask, then add 25 mL of dimethyl sulfoxide, stir to dissolve, and add 3 mL of tetrabutyl titanate , stir evenly, add 0.6 mL of saturated potassium chloride solution, heat under reflux at 120 °C for 2 h, and then separate by high-speed centrifugation to obtain 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dinitrile composite Titanium dioxide quantum dots.
[0016] The fluorescence emission map of 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile composite titanium dioxide quantum dots was scanned with excitation at 550 nm on an Agilent Cary Eclipse Fluorescence Spectrophotometer. The results are shown in figure 2 ,from figure 2 It can be seen that the 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile composite titanium dioxide quantum dots have a strong fluorescence emission signal at 606 nm.
[0017] Using the prepared 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile composite titanium dioxide quantum dots, the final concentration is 2.0mg 3-(4-amino)-butyric acid-acenaphthene Pyrazine-8,9-dinitrile complexed TiO2 quantum dots were incubated in HeLa cells at 37°C, 5% CO. 2 Incubate for 30min, then shake and rinse with PBS for 5min×3, add cell culture medium, and image by confocal laser. A representative area was selected and observed with an oil lens (100×), which was repeated three times. Imaging showed strong fluorescent signal in Hela cells, see figure 1 , the collection band is 580-620nm, and the 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile composite titanium dioxide quantum dots can present obvious fluorescence signals in cells.
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Description & Claims & Application Information

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