Application of composite fluorescent probe in imaging detection of live tumor cells

A composite fluorescence and cell imaging technology, applied in the direction of fluorescence/phosphorescence, luminescent materials, measuring devices, etc., can solve the problems of application limitations, high biological toxicity of quantum dots, etc., and achieve low biological toxicity, good cell membrane permeability, and good water solubility sexual effect

Active Publication Date: 2019-02-26
重庆问创基因科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the application of biological imaging, due to the high biological tox

Method used

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  • Application of composite fluorescent probe in imaging detection of live tumor cells
  • Application of composite fluorescent probe in imaging detection of live tumor cells
  • Application of composite fluorescent probe in imaging detection of live tumor cells

Examples

Experimental program
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[0009] Example

[0010] The synthetic route of 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile:

[0011]

[0012] Synthesis steps:

[0013] 1. Add 200 mg of 5-bromoacenaphthoquinone and 125 mg of diaminomaleonitrile to a 50 mL two-necked flask, then add 6 mL of glacial acetic acid, stir and heat under reflux at 120 °C for 2 h, cool in an ice-water bath, filter, dry, and then the column layer The bright yellow 3-bromo-acenaphthopyrazine-8,9-dicarbonitrile was obtained by analysis and separation;

[0014] 2. Take 100 mg of 3-bromo-acenaphthopyrazine-8,9-dicarbonitrile and 40 mg of 4-aminobutyric acid into a 50 mL round-bottomed flask, add 8 mL of dimethyl sulfoxide, heat under reflux at 110 °C for 1 h, the reaction generates The red mixture can be distilled under reduced pressure to obtain 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile;

[0015] 3. Add 6 mg of 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dicarbonitrile to a 50 mL two-necke...

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Abstract

The invention discloses an application of a composite fluorescent probe in imaging detection of live tumor cells. When the composite fluorescent probe is used in imaging detection of live tumor cells,in a waveband of 580 to 620 nm, the composite fluorescent probe generates prominent fluorescent signals in live tumor cells, and the composite fluorescent probe is a 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dinitrile compounded titanium dioxide quantum dot. Titanium dioxide has a strong light absorbing performance, energy absorbed by titanium dioxide is transmitted to 3-(4-amino)-butyric acid-acenaphthopyrazine-8,9-dinitrile, the emitting strength of the quantum dot is enhanced, strong fluorescent signals are obtained, organic substances are gathered on titanium dioxide nano particles,the water solubility of the quantum dots is improved, and moreover, the fluorescent probe has a good performance on penetrating the cell membranes.

Description

technical field [0001] The invention belongs to the application technical field of fluorescent probes, and in particular relates to the application of a composite fluorescent probe in the imaging detection of tumor live cells. Background technique [0002] Compared with traditional dye molecules in the field of biological imaging, quantum dots do have several advantages. Inorganic microcrystals can withstand multiple excitations and light emissions, while organic molecules will decompose. The long-lasting stability allows researchers to observe cells and tissues for a longer period of time and perform interface modification and connection without difficulty. The biggest advantage of quantum dots is that they have rich colors. The complexity of biological systems often requires simultaneous observation of several components. If dyed with dye molecules, different wavelengths of light are required for excitation, but quantum dots do not have this problem. Nanocrystals of diffe...

Claims

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Application Information

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IPC IPC(8): C07D241/38C09K11/06C09K11/67G01N21/64
CPCG01N21/6428C09K11/06C09K11/671C07D241/38C09K2211/1044
Inventor 李一达
Owner 重庆问创基因科技有限公司
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