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Seedling breeding method of dendrobium

A Dendrobium and culture medium technology, applied in the field of dendrobium seedling cultivation, can solve the problems of complicated preparation, long seedling growth cycle, complex culture medium, etc., and achieve the effects of simple culture medium, convenient preparation and shortened growth cycle

Inactive Publication Date: 2019-03-01
上海摩天农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The technical problem to be solved in the present invention is to overcome the complex culture medium, complicated preparation and long growth cycle of seedlings in the method for growing seedlings of Dendrobium in the prior art, and provide a method for raising seedlings of Dendrobium, which has a simple formula for the culture medium, The growth cycle of seedlings is shortened, the survival rate is high, and it is suitable for factory production

Method used

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  • Seedling breeding method of dendrobium
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  • Seedling breeding method of dendrobium

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Embodiment 1

[0045] The method for the tissue culture of Dendrobium candidum of embodiment 1 comprises the following steps:

[0046] (1) Selection and disinfection of explants: This method selects the full and uncracked capsules of 4-6 months old as explants, and washes them with clear water first; The surface is disinfected, then disinfected with 0.1% mercuric chloride solution, and finally rinsed with sterile water for 6 times and set aside.

[0047] (2) Sowing and germination: In the ultra-clean bench, break open the sterilized capsule of (1), spread the seeds evenly on the aseptic germination medium, and cultivate it under light for 30 days , the seeds germinate and slowly form a protocorm; cultivated to 30 days, the protocorm can be clearly distinguished as a needle-like shape with the naked eye. Seed sterile germination medium is: B 5 +Agar 8g / L+3% sucrose+pH5.8.

[0048] (3) Differentiation of the protocorm: After the protocorm is formed, inoculate the (2) protocorm on the differ...

Embodiment 6

[0072] The tissue culture of Dendrobium Huoshanense comprises the following steps:

[0073] (1) Selection and disinfection of explants: This method selects the full and uncracked capsules of 4-6 months old as explants, and washes them with clear water first; The surface is disinfected, then disinfected with 0.1% mercuric chloride solution, and finally rinsed with sterile water for 6 times and set aside.

[0074] (2) Sowing and germination: In the ultra-clean bench, the sterilized capsules of step (1) are broken open, and the seeds in it are evenly spread on the aseptic germination medium, and cultivated under light for 30 After 30 days, the seeds germinate and slowly form a protocorm; after culturing for 30 days, the protocorm can be clearly distinguished as a needle-like shape with the naked eye. Seed sterile germination medium is: B 5 +Agar 8g / L+2.5% sucrose+pH6.0.

[0075](3) Differentiation of the protocorm: after the protocorm is formed, the (2) protocorm is inoculated...

Embodiment 7

[0082] The tissue culture of Dendrobium drumsticks comprises the following steps:

[0083] (1) Selection and disinfection of explants: This method selects the full and uncracked capsules of 4-6 months old as explants, and washes them with clear water first; The surface is disinfected, then disinfected with 0.1% mercuric chloride solution, and finally rinsed with sterile water for 6 times and set aside.

[0084] (2) Sowing and germination: In the ultra-clean bench, the sterilized capsules of step (1) are broken open, and the seeds in it are evenly spread on the aseptic germination medium, and cultivated under light for 30 After 30 days, the seeds germinate and slowly form a protocorm; after culturing for 30 days, the protocorm can be clearly distinguished as a needle-like shape with the naked eye. Seed sterile germination medium is: B 5 +Agar 8g / L+3.5% sucrose+pH5.8.

[0085] (3) Differentiation of the protocorm: after the protocorm is formed, the (2) protocorm is inoculated...

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Abstract

The invention discloses a seedling breeding method of dendrobium. The seedling breeding method comprises the following steps that (1) cluster buds of the dendrobium are inoculated in a proliferation subculture medium to carry out proliferation subculturing, and seedlings are formed, the proliferation subculture medium is prepared from MS, 0.1-2.0mg / L of NAA, and 2.0-3.5% of sucrose; and (2) the seedlings are inoculated in a seedling strengthening and root induction medium to carry out seedling strengthening and root induction culture, the seedling strengthening and root induction medium is prepared from MS, 0.1-2.0mg / L of NAA and 2.0-3.5% of sucrose. The specific medium system is used in different culture stages, through the seedling breeding method of the dendrobium, annualized and factory production of tissue culture seedlings can be realized without the influence of seasons and environment; seeds germinate early, the growth cycle of seedling breeding is shortened; and the seeds arebred for 5-6 months, the rooting rate can reach 65% or above, and the plant height can reach 2-6 cm.

Description

technical field [0001] The invention relates to a method for raising seedlings of dendrobium. Background technique [0002] Dendrobium plants are all epiphytic plants. Their growth environment is unique and they have very strict requirements on the climate environment. There are trees on the side for shade, and there are streams and ditches below. When there is severe water shortage, the leaves often fall off. Dendrobium officinale, Dendrobium Huoshanense, and Dendrobium chrysanthemum have strict requirements on growth conditions, and their own reproductive ability is poor, which greatly limits their promotion and application. For example, Dendrobium officinale is occasionally seen in the wild in Yunnan, Guizhou and Sichuan, but it is endangered and its own reproductive capacity is extremely low. The fruit of Dendrobium officinale is a capsule, which is generally long, pear-shaped or oval. Each fruit has tens of thousands of seeds, but the seeds are extremely small, endosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李付忠董丽菊伍淼
Owner 上海摩天农业科技有限公司
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