Octavinyl-POSS and ethylene glycol dimethacrylate co-crosslinked boron affinity monolithic column and preparation method thereof

A technology of ethylene glycol dimethacrylate and octavinyl, which is applied in the field of boron affinity monolithic column and its preparation, can solve the problems of lack of specificity in separation effect, and achieve easy operation, good permeability and recovery rate Improved effect

Active Publication Date: 2019-03-01
TIANJIN MEDICAL UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, solid-phase microextraction lacks specificity for the separation of complex samples, so

Method used

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  • Octavinyl-POSS and ethylene glycol dimethacrylate co-crosslinked boron affinity monolithic column and preparation method thereof
  • Octavinyl-POSS and ethylene glycol dimethacrylate co-crosslinked boron affinity monolithic column and preparation method thereof
  • Octavinyl-POSS and ethylene glycol dimethacrylate co-crosslinked boron affinity monolithic column and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Comparison of solid-phase microextraction recoveries of octavinyl-POSS and ethylene glycol dimethacrylate co-crosslinked monolithic columns with monolithic columns without octavinyl-POSS crosslinking agent. Specific steps are as follows:

[0029] Preparation of boron-affinity monolithic columns co-crosslinked with octavinyl-POSS and ethylene glycol dimethacrylate by in situ polymerization:

[0030] The mass percent crosslinking agent octavinyl-POSS 1.63% and ethylene glycol dimethacrylate 4.05%, functional monomer 3-acrylamidophenylboronic acid 3.08%, initiator azobisisobutyronitrile 0.26%, dissolved In the mixed porogen solution of 9.00% dimethyl sulfoxide and 81.98% chloroform as the porogen; ultrasonic (power 150W) was used to dissolve for 30 minutes to dissolve and clarify it, and then nitrogen gas was introduced to remove the porogen in the pre-polymerization solution. Oxygen, then inject the pre-polymerization liquid into the clean capillary, seal the two ends wi...

Embodiment 2

[0035]In solid-phase extraction, the adsorption of the target compound must go through four steps: activation, sample loading, rinsing, and elution, and the elution link is very important. The purpose of elution is to wash off the specifically adsorbed target molecules, and then evaluate the adsorption recovery effect according to the eluted amount of the target molecules. In order to clarify the impact of the concentration of the boron affinity monolithic column eluent on octavinyl-POSS and ethylene glycol dimethacrylate co-crosslinking on the recovery rate of horseradish peroxidase solid-phase microextraction, the present invention has different Concentration of the eluate was screened. The specific operation steps are as follows:

[0036] a. Preparation of monolithic column: Synthesize a boron-affinity monolithic column co-crosslinked with octavinyl-POSS and ethylene glycol dimethacrylate by the same method as above (Example 1).

[0037] b. Elution process: use 100 mL met...

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Abstract

The invention relates to anoctavinyl-POSS and ethylene glycol dimethacrylate co-crosslinked boron affinity monolithic column and a preparation method thereof. The octavinyl-POSS ethylene glycol dimethacrylate co-crosslinked boron affinity monolithic column is prepared from 1.63 percent of octavinyl-POSS, 4.05 percent of ethylene glycol dimethacrylate, 0.26 percent of azobisisobutyronitrile, 3.08 percent of 3-acryloylaminophenylboronic acid, 81.98 percent of chloroform and 9.00 percent of dimethyl sulfoxide. The boron affinity monolithic column is prepared by co-crosslinking octavinyl-POSS andethylene glycol dimethacrylate, and is applied to solid-phase microextraction of horse radish peroxidase. Compared with aboron affinity monolithic column without the octavinyl-POSS, the boron affinitymonolithic column containing a proper amount of octavinyl-POSS as a crosslinking agent has the advantages that the recovery rate during solid-phase microextraction of the horse radish peroxidase is improved twice or more, being 90 percent or more, and the relative standard deviation (RSD) is less than 5 percent.

Description

technical field [0001] The invention relates to a boron affinity monolithic column co-crosslinked with octavinyl-POSS and ethylene glycol dimethacrylate and a preparation method thereof. Background technique [0002] Glycoproteins are an important class of post-translationally modified proteins. They are substances in which oligosaccharide chains and polypeptides are covalently linked in various forms and have biological activity. They have both the functional properties of polysaccharides and proteins. There are many kinds of glycoproteins in complex and diverse forms, but their content is low. With the development of glycobiology and modern separation and purification technology, research on the extraction, purification, separation and enrichment of glycoproteins has become the focus of many researchers. There are many enrichment methods for glycoproteins. The lectin technique is simple to operate and has good reproducibility, but the cost of lectins is high and the specif...

Claims

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Application Information

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IPC IPC(8): C08F283/12C08F222/14C08F230/06C08J9/28C08L51/06B01J20/26B01J20/30
CPCC08F283/124C08J9/28B01J20/261C08J2201/0424C08J2351/08C08F222/102C08F230/06
Inventor 刘照胜张雪申艳凤黄艳萍
Owner TIANJIN MEDICAL UNIV
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