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Application of SlTOE 1 gene in regulating and controlling flowering time and yield of tomatoes

A yield and gene expression technology, applied in the field of plant genetic engineering and genetic modification, can solve the problem that gene expression cannot be completely inhibited

Active Publication Date: 2019-03-05
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology does not completely inhibit gene expression, but only partially inhibits its expression

Method used

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  • Application of SlTOE 1 gene in regulating and controlling flowering time and yield of tomatoes
  • Application of SlTOE 1 gene in regulating and controlling flowering time and yield of tomatoes
  • Application of SlTOE 1 gene in regulating and controlling flowering time and yield of tomatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the acquisition of the plant binary expression vector of SlTOE1 gene silencing

[0056]The plant binary recombination vector pBIN19::SlTOE1i (SlTOE1::RNAi) for SlTOE1 gene silencing replaces the pBIN19 plasmid with the SlTOE1 hairpin silencing unit shown in Sequence 3 (recorded in Bevan M.Binary Agrobacterium vectors for plant transformation.Nucleic Acids Res.1984.12 (22): 8711-8721., the public can obtain from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) the fragment between the Sac I and Xba I restriction sites, the vector obtained, and through the CaMV 35S promoter (sequence 3 pp. 1012-1357) drive expression.

[0057] The SlTOE1 hairpin silencing unit shown in sequence 3, wherein, the 1366-1865th position of the sequence 3 is the 209-708th position of the SlTOE1 gene sequence 1, and the 2677-3176th position of the sequence 3 is the reverse of the 209-708th position of the SlTOE1 gene sequence 1 To the complementary ...

Embodiment 2

[0072] Example 2, the acquisition of SlTOE1 gene silencing transgenic tomato and the genetic regulation of flowering time and yield

[0073] 1. Obtaining transgenic tomato with SlTOE1 gene silencing

[0074] 1. Obtaining recombinant bacteria with SlTOE1 gene silencing

[0075] The recombinant expression vector SlTOE1::RNAi transformed into Agrobacterium AGLO by the SlTOE1 gene silencing obtained in Example 1 (recorded in Li C, Liu G, Xu C, Lee GI, Bauer P, Ling HQ, Ganal MW, Howe GA.The tomatosuppressor of prosystemin-mediated responses2gene encodes a fatty acid desaturase required for the biosynthesis of jasmonic acid and the production of a systemic wound signal for defense gene expression. Plant Cell. 2003.15(7):1646-1661. Obtained from the Institute of Biosciences), single clones were picked, and cultured overnight at 28°C with shaking in LB liquid medium containing kanazyme and rifampicin antibiotics to obtain transformants.

[0076] The transformants were identified by...

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Abstract

The invention discloses application of an SlTOE1 gene in regulating and controlling flowering time and yield of tomatoes. The invention provides application of any one of 1) a protein SlTOE 1, 2) a DNA molecule for coding the protein SlTOE 1, and 3) a recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing the DNA molecule for coding the protein SlTOE 1 in regulating and controlling flowering time and yield of a plant. The SlTOE1 gene is found to be related to the flowering time and the yield of tomatoes, and the SlTOE1 protein and the partial coding gene thereof can be used for cultivation and identification of early-flowering and high-yield plant varieties, and have an important effect in the field of yield breeding and plant flowering time, and the effects of the early flowering and high yield of the tomatoes are promoted.

Description

technical field [0001] The invention belongs to the fields of plant genetic engineering and genetic modification, and in particular relates to an application of SlTOE1 gene in regulating tomato flowering time and yield. Background technique [0002] At present, the problem of food security is becoming more and more urgent, and cultivating high-yielding crop varieties is the fundamental measure to solve the problem of food security. Increasing per unit yield can effectively increase land utilization rate, reduce land use, and meet people's growing demand. Flowering is an extremely important physiological process in most plants. Regulating the flowering time of plants is beneficial to avoid the peak period of maturity, realize greater economic value, and expand the planting range. For example, early-maturing varieties can adapt to areas with a shorter frost-free period. Breeding early maturing and high yielding varieties is an important goal of many crop breeding. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N15/113
CPCC07K14/415C12N15/113C12N15/8261C12N15/827Y02A40/146
Inventor 李传友朱强邓磊李常保蒋红玲
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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