Microbial preparation method of suberogorgin

A microbial and corallic acid technology, applied in the field of microorganisms, can solve the problems of asymmetric synthesis, no report on the preparation of gorgonian acid by microorganisms, and cumbersome steps of gorgonian acid

Active Publication Date: 2019-03-08
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This synthetic route is known as the successful model of classic gorgonian acid synthesis, but the yield is not high
Looking at all the total synthesis to obtain gorgonian acid, the steps are cumbersome and require asymmetric synthesis, which brings extremely unfavorable factors to its real industrial production
So far, there is no report on the microbial preparation of gorgonian acid

Method used

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  • Microbial preparation method of suberogorgin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] An embodiment of the microbial preparation method of gorgonian acid of the present invention.

[0043] (1) Strain activation: use an inoculation loop to take an appropriate amount of seeds from the EGF15-0-3 strain storage tube, inoculate into a 100mL triangular conical flask containing 50mL PDA liquid medium, and shake at a constant temperature of 25-28°C. Cultivate in the bed at a speed of 150-170 rpm for 2-3 days to obtain a seed culture solution of EGF15-0-3;

[0044] (2) Strain fermentation: the above-mentioned activated seed culture solution was transferred to different fermentation media in an amount of 1.5%; the fermentation media were 8 kinds of liquid culture media, 4 kinds of solid media and 11 kinds of biotransformation culture 2L for each culture medium, and 2L for each blank culture medium; various cultures are based on static culture at 25-28°C for 48-60 days, observe and record the growth status of the strains during the culture period, liquid and The g...

Embodiment 2

[0063] An embodiment of post-treatment of metabolites in the microbial preparation method of gorgonian acid in the present invention.

[0064] The flow chart of post-treatment of secondary metabolites in different media is as follows: image 3 shown. After the bacterial strains of each fermentation medium in Example 1 are inactivated, the liquid medium culture solution is filtered with gauze, and the fermentation solution and mycelia are separated; , n-BuOH (n-butanol) extraction three times, the extract obtained is concentrated, and finally the fermented liquid EtOAc, n-BuOH phase extract is obtained; the mycelium is extracted 3 times with methanol (1 time / day), and the methanol is combined The extract was concentrated under reduced pressure to obtain methanol extract, which was dissolved in water and then extracted three times with equal volumes of EtOAc and n-BuOH in the same way to obtain mycelium EtOAc and n-BuOH phase extract. An equal volume of EtOAc was added to the ...

Embodiment 3

[0065] Example 3 Separation and structure identification of gorgonian acid

[0066] 1. Isolation of Gorniconic Acid

[0067] Use an inoculation loop to take an appropriate amount of seed solution from the EGF15-0-3 strain preservation tube, and inoculate it into 50mL PDA medium (potatoes 200g / L, glucose 2.0%, potato juice prepared with old seawater as a solvent, natural pH) In a 100mL Erlenmeyer flask, cultured in a shaker at 28°C at 165r / min for 2 days to obtain a seed culture solution of EGF15-0-3. Draw 1.5mL of the seed solution and add it to a 1000mL Erlenmeyer flask filled with 500mL D medium (potatoes 200g / L, glucose 2.0%, potato juice prepared with aged sea water, natural pH), and culture at 28°C for 60 days , compared with the blank medium to observe whether there is obvious strain growth, stop the fermentation after the growth is completed, and separate and co-ferment 220L of bacterial liquid.

[0068]The fermentation broth is filtered with gauze to obtain two parts...

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Abstract

The invention relates to a microbial preparation method of suberogorgin, and belongs to the technical field of microorganisms. The preservation number of a biological bacterium (Aspergillus sp.) usedin the method is GDMCC No.60476. The microbial preparation method of suberogorgin is provided for the first time based on the method for preparing suberogorgin from the biological bacterium provided by the invention; it is confirmed for the first time that a true source of suberogorgin is produced by co-existing microorganisms, and the source problem is solved really; a growth environment is regulated based on a medium for the first time, and the influence of eutrophication and biotransformation on the production of suberogorgin is observed; the suberogorgin is isolated from alcyonacea co-existing fungi for the first time, and a hypothesis that secondary metabolites produced by a host are often produced by co-existing, endogenous or environmental microorganisms is proved.

Description

technical field [0001] The invention relates to a microbial preparation method of gorgonian acid, which belongs to the technical field of microorganisms. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory, cognitive impairment and personality changes. AD is the most common cause of Alzheimer's disease, accounting for 60% to 80%. At present, the number of AD patients in my country has reached 6 million. With the intensification of population aging in my country, the incidence rate will gradually increase. It is estimated that there are currently 50 million Alzheimer's patients in the world, and it is expected to reach 82 million in 2030 and 152 million in 2050. From 2000 to 2013, deaths from stroke, heart disease, and prostate tumors decreased by 23%, 14%, and 11%, respectively, while deaths from AD increased by 71%. At the same time, patients lose their personal life and behavioral capabilities, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C12N1/14C12R1/66
CPCC12N1/14C12P17/02
Inventor 张翠仙韦霞刘炳新冯婵
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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