Method for extracting polyunsaturated fatty acid from marine microalgae
A technology of unsaturated fatty acids and marine microalgae, which is applied in the field of extracting polyunsaturated fatty acids, can solve the problems of lack of universal applicability, and achieve the effects of low cost, convenient operation and simple preparation process
Pending Publication Date: 2019-03-15
QINGDAO UNIV
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However, this study is only aimed at the extraction of polyunsaturated fatty acids in Chlorella, which lacks general applicability
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[0020] The process of extracting polyunsaturated fatty acids in this embodiment comprises the following steps:
[0021] (1) Get 50 g of Schizochytrium algae powder, add 1100 mL of chloroform:methanol=1.2:1 solution;
[0022] (2) leaching at 35°C and ultrasonic (working frequency 40kHz, ultrasonic power 100W) for 7.5h to obtain the lipids in the algae powder;
[0023] (3) After filtering the liquid after ultrasonic leaching, transfer it to a separatory funnel, vibrate the separatory funnel for 3 minutes, and let stand overnight;
[0024] (4) When the upper and lower separation liquid levels are obvious and the upper layer is clear, collect the lower organic solution in a round-bottomed flask, and evaporate it in a vacuum rotary water bath at 38.5 ° C, and wash it with chloroform for 2-3 times after evaporation to dryness. The fatty acid obtained after evaporation to dryness was stored at -40°C; as shown in Table 1, the main polysaturated fatty acid in the Schizochytrium algae ...
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The invention belongs to the technical field of marine biological extraction and relates to a method for extracting a polyunsaturated fatty acid from marine microalgae. The method comprises the following steps: firstly adding marine microalgae powder into a mixed solution of chloroform and methanol, then performing ultrasonic leaching and extraction, filtering obtained liquid, transferring filtered liquid to a separating funnel, collecting an organic solution at the lower layer when the separation of the upper liquid level and the lower liquid level is obvious and the upper layer is clarified,placing the collected organic solution in round-bottom flask, evaporating to dryness in water bath, washing with chloroform, then transferring the round-bottom flask to a screw-top glass tube, and blow-drying chloroform with nitrogen to obtain lipid; then adding methanol and urea into the obtained lipid for enrichment and clathration reaction; quickly filtering the reaction product after clathration is finished, taking filtrate, subjecting the filtrate to rotary evaporation to remove methanol, adding hydrochloric acid into the filtrate to maintain an acidic environment, adding n-hexane to extract the upper layer, then removing impurities with a sodium chloride solution, taking the upper layer until the upper layer is neutral; and finally adding anhydrous sodium chloride to dry, thereby obtaining the high-purity polyunsaturated fatty acid. The preparation process is simple, convenient to operate and low in cost, and the extracted polyunsaturated fatty acid is high in purity.
Description
Technical field: [0001] The invention belongs to the technical field of marine organism extraction, in particular to a method for extracting polyunsaturated fatty acids from marine microalgae. Background technique: [0002] Marine algae is an important marine resource, rich in a variety of fatty acids, especially polyunsaturated fatty acids (PUFAs). Polyunsaturated fatty acids in lipid products, especially n-3 polyunsaturated fatty acids (n-3PUFAs), play an important role in maintaining human nutrition balance and regulating human chronic diseases, and are one of the necessary nutrients to maintain human health. With the development of industry, the intake of n-3 polyunsaturated fatty acids by human beings is generally small today, so corresponding high-concentration fatty acid supplements are prepared to meet people's needs. [0003] At present, in my country's large-scale industrial production, fatty acids, especially polyunsaturated fatty acids, basically come from deep-...
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IPC IPC(8): C11B1/10C11C1/02
CPCC11B1/106C11C1/02
Inventor 李铎李珊李克磊郭晓飞赵峰
Owner QINGDAO UNIV