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Microfluidic device and method for detecting content of macromolecules in non-Newtonian fluid

A non-Newtonian fluid and microfluidic device technology, applied in chemical instruments and methods, laboratory containers, analytical materials, etc., can solve the problems of high requirements for fluorescein and complicated operation, and achieve simple operation, simple method, Easy to make effects

Active Publication Date: 2019-03-19
XI AN JIAOTONG UNIV
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Problems solved by technology

However, the disadvantages of this method are: 1) Fluorescent labeling of macromolecules has higher requirements for fluorescein
2) It is difficult to fluorescently label some polymers
3) The operation during the chemical reaction is more complicated

Method used

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Embodiment Construction

[0023] Below in conjunction with accompanying drawing and specific embodiment the present invention will be described in further detail:

[0024] Such as Figure 1 to Figure 3 As shown, the present invention is a microfluidic device for detection of high molecular content in non-Newtonian fluids. The device is processed by standard soft lithography. The slide layer 9 is composed of; the cover sheet layer 8 is sequentially connected with a target sample solution adding area 1, an entrance observation area 2, an entrance contraction area 3, a tapered microchannel 4, an exit expansion area 5, and an exit observation area 6 and the microchannel formed by the liquid discharge area 7; the sample liquid addition area 1, the entrance observation area 2, the entrance contraction area 3, the tapered microchannel 4, the outlet expansion area 5, the exit observation area 6 and the liquid discharge area 7 is located at the central position where the cover sheet layer 8 and the slide 9 are...

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Abstract

The invention provides a microfluidic device and method for detecting the content of macromolecules in non-Newtonian fluid. The device is composed of a cover glass layer and a slide glass layer, wherein the cover glass layer is provided with a target sample solution feeding region, an inlet observation region, an inlet contraction region, a gradual contraction micro-channel, an outlet expanding region, an outlet observation region and a liquid discharging region, wherein micro-particles are diluted through a macromolecule solution to be detected and then enter the variable-section micro-channel; after the particles pass through a gradual contraction micro-channel structure, the particles have different enrichment degrees at a channel outlet due to different macromolecule concentrations inthe non-Newtonian fluid; bandwidth variance of the particles under different flow speeds can be calculated through a particle distribution image obtained from the outlet; and the obtained bandwidth variance is compared with a standard database obtained by an experiment, so as to obtain the macromolecule concentration in the fluid. The microfluidic device has the advantages of easiness for processing, simple structure, low cost, convenience for carrying, high adaptability and the like, and has a very good application prospect in biomedicines, food science and production and livings.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a microfluidic device and method for detecting high molecular content in non-Newtonian fluids. Background technique [0002] Fluids that satisfy Newton's law of internal friction between shear stress and shear rate are called Newtonian fluids, and fluids that do not satisfy Newton's law of internal friction between shear stress and shear rate are called non-Newtonian fluids. Such as blood and milk are typical non-Newtonian fluids. Polymers are usually present in such non-Newtonian fluids. The presence of polymers fundamentally changes the relationship between shear stress and shear rate in fluids. The rapid and portable detection of these high molecular content is of great significance in the fields of biomedicine, clinical diagnosis and food safety. For example, in the field of food safety, detecting the protein polymer content in milk can conveniently ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N15/00
CPCB01L3/5027B01L2200/10B01L2300/0861B01L2300/0887B01L2300/12G01N15/00
Inventor 赵亮吴旭范亮亮陶亦毅
Owner XI AN JIAOTONG UNIV
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