Upconverting nanoparticle-based non-oxygen-dependent photodynamic diagnosis and treatment probe and preparation method

Abstract

The invention belongs to the technical field of organic compounds, and discloses an upconverting nanoparticle-based non-oxygen-dependent photodynamic diagnosis and treatment probe and a preparation method. The upconverting nanoparticle-based non-oxygen-dependent photodynamic diagnosis and treatment probe consists of modified upconverting nanoparticles and azo-compounds, wherein the modified upconverting nanoparticles consists of positively charged modified upconverting nanoparticles and negatively charged modified upconverting nanoparticles; the azo-compounds are organic molecules containing nitrogen-nitrogen double bonds which can be photodecomposed under ultraviolet light; and the azo-compounds consists of a negatively charged azo-compound and a positively charged azo-compound. Accordingto the upconverting nanoparticle-based photodynamic probe disclosed by the invention, a process of photodynamic treatment thereof can be triggered by near-infrared light, overcoming the problem thatthe treatment depth of a conventional photodynamic treatment is insufficient due to the direct use of ultraviolet / visible light source. Meanwhile, under the irradiation of the near-infrared light, theprobe can have near-infrared II luminescence, which can be used for the in-vivo gathering and metabolism monitoring of the probe as well as the location of tumor tissues before treatment.

Description

technical field [0001] The invention belongs to the technical field of organic compounds, in particular to an upconversion nanoparticle non-oxygen-dependent photodynamic diagnosis and treatment probe and a preparation method. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: At present, the clinical treatment methods for tumors are mainly three categories: radiotherapy, chemotherapy and surgery. The characteristics have gradually attracted attention, and its main mechanism is that the photosensitizer absorbs the excitation light to generate reactive oxygen species, thereby damaging the protein or DNA of tumor cells, and then achieving the purpose of inhibiting tumors. At present, in addition to applying photosensitizer drugs alone in tumor treatment, more photosensitizers are combined with nanocarriers to improve the depth and targeting of treatment by constructing a composite probe. Common nanocarriers include...

AI Technical Summary

Problems solved by technology

However, photosensitizers need to rely on partial pressure of oxygen in the process of photodynamic therapy, and the hypoxic environment of tumors greatly reduces their curative effect; most of the excitation light wavelengths used in clinical photosensitizers are in the visible light region, due to the scattering and absorption of short-wavelength biological tissues Strong, so the treatment depth is greatly limited, and the traditional photosensitizers have poor photostability due to the destruction of the conjugated system and the blockage of electronic transitions due to their conjugated structures under long-term illumination. needle application

[0003] To sum up, the problems existing in the prior art are: photosensitizers need to rely on partial pressure of oxygen in the process of photodynamic therapy, and the hypoxic environment of tumors greatly reduces its curative effect; the treatment depth of traditional photosensitizers is low, and it is difficult to achieve deep treatment. Penetration and poor photostability of traditional photosensitizers limit the application of photodynamic nanoprobes

[0005] How to solve the problems of tumor hypoxia inhibition, low treatment depth and poor stability in the process of traditional photosensitizer therapy is the key to improving the effect and application of photodynamic therapy

The hypoxic microenvironment of the tumor due to its vigorous metabolism is severely inhibited by clinical oxygen-dependent photodynamic therapy, resulting in very limited PDT treatment effect. Therefore, how to overcome or avoid the hypoxic inhibition process is the key to achieving efficient PDT treatment of tumors

The photosensitizers used in traditional PDT treatment have poor photostability, and the excitation light source used has poor biological tissue penetration and severe oxygen dependence, which limits its clinical application

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