Detection method of four forbidden fluoroquinolone drug residues in aquaculture water

Abstract

The invention provides a detection method of four forbidden fluoroquinolone drug residues in aquaculture water, and belongs to the technical field of environment quality security detection. The methodcomprises the following steps: taking 50mL of a water sample, adding an acetic acid-acetonitrile solution with the same volume to extract, and shaking out for 30min; adding an appropriate amount of sodium chloride, mixing for 1min, and standing for layering; taking 25mL of supernate, and concentrating, namely blowing the supernate with nitrogen at 40 DEG C till to nearly dry; adding 1mL of 0.1% formic acid-water-methanol (90+10) to re-dissolve, filtering through a filter membrane of 0.22 micron for future detection. The detection method disclosed by the invention has the advantages that the pretreatment for detecting the residues of ofloxacin, pefloxacin, lomefloxacin and norfloxacin in the aquaculture water is simple and convenient, the liquid chromatogram-tandem mass spectrometry and multiple reaction monitoring modes are adopted, the determination on the nature and the quantitation is accurate, the sensitivity is high, the reproducibility is good. Theoretical support of the detection method is provided for formulating related industry standard by the nation.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and specifically relates to a liquid chromatography-mass spectrometry detection method for the residues of four banned fluoroquinolones, ofloxacin, pefloxacin, lomefloxacin, and norfloxacin in aquaculture water method. Background technique [0002] Fluoroquinolone drugs are a series of artificially synthesized 4-quinolone core structures, a class of broad-spectrum antibacterial drugs that are substituted by fluorine on the 6-position C of the core core. They have strong antibacterial effects and are effective against both Gram-positive and negative bacteria. effect. It has the characteristics of fast absorption, wide distribution in the body, high blood concentration, long plasma half-life, simple synthesis, and low drug price. It is often used in the treatment of infectious diseases in animals and humans. In recent years, with the development of my country's aquaculture indust...

AI Technical Summary

Problems solved by technology

Announcement No. 2292 of the Ministry of Agriculture of the People's Republic of China in 2017 issued various salts, esters and various preparations of the four APIs of ofloxacin, pefloxacin, lomefloxacin, and norfloxacin, which may be harmful to the breeding industry, Hazard to human health or potential risk

At present, there is no standard method for the determination of fluoroquinolone drug residues in aquaculture water in China. Therefore, a method for the detection of four banned fluoroquinolone drug residues in aquaculture water can be developed to detect ofloxacin and pefloxacin. , lomefloxacin and norfloxacin are banned in aquaculture and play a role in risk monitoring

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