Method for rapidly detecting buffalo fasciola spp antibody
A detection method and buffalo technology, applied in the field of cattle blight detection, can solve the problems of high technical requirements for operation, unsuitable for popularization and use, time-consuming, etc., achieving good application prospects, promoting monitoring and purification work, and rapid detection.
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[0044] Early preparation:
[0045] Materials: bovine serum albumin (BSA), tetrachloroauric acid (Sigma), analytically pure trisodium citrate, goat anti-mouse IgG secondary antibody, 135 nitrocellulose membrane (NC membrane), glass fiber paper OB6, ordinary absorbent paper, PVC bottom plate.
[0046] 1. Anti-buffalo IgG monoclonal antibody 2G7
[0047] 1. Purification of anti-buffalo IgG monoclonal antibody 2G7, the specific steps are as follows:
[0048] 1) Take the ascites produced by injecting the hybridoma cell line 2G7 into BaLb / C mice, and centrifuge at 4°C and 12000rpm for 15min to remove impurities;
[0049]2) Mix acetate buffer solution with mouse ascites at a ratio of 2:1, stir at room temperature, and add n-octanoic acid dropwise (33 μL n-octanoic acid per ml of ascitic fluid), mix at room temperature for 30 minutes to obtain a mixture ;
[0050] 3) Let the mixed solution stand at 4°C for at least 2 hours to allow the mixed solution to fully precipitate;
[0051...
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