Identification method for unearthed cooked wheaten food cultural relic

An identification method, pasta technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low technical accuracy and difficult preservation of markers, and achieve the effect of improving accuracy and success rate

Inactive Publication Date: 2019-03-26
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
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Problems solved by technology

[0004] Purpose of the invention: an identification method for unearthed pasta cultural relics to solve the problems of low accuracy of traditional pasta cultural relics identification technology and difficult preservation of markers

Method used

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  • Identification method for unearthed cooked wheaten food cultural relic
  • Identification method for unearthed cooked wheaten food cultural relic
  • Identification method for unearthed cooked wheaten food cultural relic

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Experimental program
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Embodiment Construction

[0022] Specific identification case 1: An identification method for unearthed pasta cultural relics:

[0023] 1 Cut the colored area of ​​the gel containing the extract of the unearthed pasta cultural relic sample into multiples of about 1mm 2 The small pieces were decolorized twice with 25 mM ammonium bicarbonate and 50% acetonitrile respectively (1 h each time), and then 500 μL of 100% acetonitrile was added for dehydration. The sample was incubated at 56° C. for 60 min in 25 mM ammonium bicarbonate buffer containing 200 μL of dithiothreitol (10 mM) to break the disulfide bonds in the sample. Then the samples were incubated in 25 mM ammonium bicarbonate buffer containing 200 μL iodoacetamide (55 mM) at room temperature in the dark for 45 min to alkylate cysteine ​​in the samples. Then, the samples were washed twice with 25 mM ammonium bicarbonate, and dehydrated again with 500 μL of acetonitrile. The sample was placed in a trypsin solution (concentration 10 ng / μL, solvent ...

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Abstract

The invention belongs to the technical field of technological protection of cultural relics and in particular relates to an identification method for an unearthed cooked wheaten food cultural relic. The method comprises the following steps: firstly, carrying out pre-treatment on the unearthed cooked wheaten food cultural relic; then desalting a treated sample through a solid-phase extraction column and drying in vacuum; then dissolving the sample into a buffering solution; carrying out centrifugal treatment and collecting supernatant; carrying out high performance liquid chromatography detection; then carrying out tandem mass spectrum analysis; and finally, selecting a protein database and searching. By adopting the identification method for the unearthed cooked wheaten food cultural relic, provided by the invention, raw material plants and fermentation microorganisms for producing cooked wheaten foods are deduced according to the identified source of residual protein, so that the identification of unearthed cooked wheaten foods is realized. The accuracy and success rate of identification are obviously improved.

Description

technical field [0001] The invention belongs to the technical field of scientific and technological protection of cultural relics, and in particular relates to an identification method for unearthed pasta cultural relics. Background technique [0002] Pasta is a food made from grain flour through fermentation and other processes. Its history can be traced back to the Neolithic Age. The unearthed pasta cultural relics are mostly found inside the unearthed ceramics and metal ware. After long-term preservation, its appearance and the substances contained in it will change obviously, and the degree of change is related to the burial environment and age. If you want to determine whether the cultural relics in a certain unearthed container are ancient pasta, you need to use scientific means to identify them, so as to provide basic information for the study of ancient Chinese food culture, plant domestication, ancient recipes, and social structure. [0003] The method proposed by...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 朱展云刘柳杨军昌
Owner NORTHWESTERN POLYTECHNICAL UNIV
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